Gene Mutants - Detail

ME No. ME9274
Strain Name AQ3588
Sex F+
Plasmid Integrated
Plasmid Autonomous F'
Prophage
Genotype endA1 hsdR17 gyrA96 thi Δ(lac-pro) recA1 supE44 relA1
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Gene Mutants Detail Info
Reference Cao Y, Rowland RR, Kogoma T.
DNA polymerase I and the bypassing of RecA dependence of constitutive stable DNA replication in Escherichia coli rnhA mutants.
J Bacteriol  (1993)  175(22)   7247-53  
[PubMed ID = 8226671]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as JM109: Yanisch-Perron et al., 1985, Gene, 33, 103-119.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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