Gene Mutants - Detail

ME No. ME9273
Strain Name AQ3580
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) tnaA::Tn10 dnaA5
Parent Donor AQ634
Parent Recipient AQ3408
Methods TD./PI
Latest Marker Ts, dnaA5
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Cui T, Moro-oka N, Ohsumi K, Kodama K, Ohshima T, Ogasawara N, Mori H, Wanner B, Niki H, Horiuchi T.
Escherichia coli with a linear genome.
EMBO Rep  (2007)  8(2)   181-7  
[PubMed ID = 17218953]  [RRC reference] 
Hong X, Cadwell GW, Kogoma T.
Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase.
Mol Microbiol  (1996)  21(5)   953-61  
[PubMed ID = 8885266]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Ts
Request
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