Gene Mutants - Detail

ME No. ME9174
Strain Name AQ1553
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 metB1 argH thyA rpoB340 deoB (or deoC) metD88 Δlac-3 rnhA224 Δ(srlR-recA)306 srlR::Tn10
Parent Donor AQ633
Parent Recipient AQ699
Methods TD./PI
Latest Marker UV-s, Δ(srlR-recA)306
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Subia NL, Kogoma T.
Concatemer formation of ColE1-type plasmids in mutants of Escherichia coli lacking RNase H activity.
J Mol Biol  (1986)  189(3)   389-99  
[PubMed ID = 3023625]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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