Gene Mutants - Detail

ME No. ME9167
Strain Name AQ1363
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 metB1 dnaA5 dasF377 thyA deoB/C asnB::Tn5 argH metD88 rpoB340 ilv-192
Parent Donor TC377
Parent Recipient DK175
Methods
Latest Marker Ls, dasF377
Selection Pro+
Source
Gene Mutants Detail Info
Reference Ogawa T, Pickett GG, Kogoma T, Kornberg A.
RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro.
Proc Natl Acad Sci U S A  (1984)  81(4)   1040-4  
[PubMed ID = 6322184]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml).
Other Remarks Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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