Ogawa T, Pickett GG, Kogoma T, Kornberg A.
RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro.
Proc Natl Acad Sci U S A
(1984)
81(4)
1040-4
[PubMed ID = 6322184]
[RRC文献情報]
培地条件
Recommended medium: M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml).
その他備考
Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044. Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
