Gene Mutants - Detail

ME No. ME9157
Strain Name AQ1130
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype asnA31 asnB50::Tn5 Δlac ara thi gyrA
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source from A. Wright
Reference Kogoma T, von Meyenburg K.
The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.
EMBO J  (1983)  2(3)   463-8  
[PubMed ID = 11894964]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as R2D2: Felton etal., 1980, J. Bacteriol., 142, 221-228.
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