Gene Mutants - Detail

ME No. ME9156
Strain Name AQ1065
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 metB1 argH thyA rpoB340 deoB (or deoC) proA3 metD88 Δlac-3 ilv-192 dnaA5
Parent Donor 740
Parent Recipient AQ1034
Methods TD./PI
Latest Marker Ts, dnaA5
Selection Suc+
Source
Gene Mutants Detail Info
Reference Kogoma T, von Meyenburg K.
The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.
EMBO J  (1983)  2(3)   463-8  
[PubMed ID = 11894964]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as DK65
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