Gene Mutants - Detail

ME No. ME9144
Strain Name AQ751
Sex Hfr
Plasmid Integrated F Hfr (serA-<-lysA)
Plasmid Autonomous
Genotype Hfr (serA-<-lysA) thi-1 argA21 drm-3 recA200 srlC300::Tn10
Parent Donor AQ749
Parent Recipient AQ270
Methods TD./PI
Latest Marker UV(Ts), recA200
Selection Tc-r
Reference Kogoma T, Hong X, Cadwell GW, Barnard KG, Asai T.
Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase HI and exonuclease V activities.
Biochimie  (1993)  75(1-2)   89-99  
[PubMed ID = 8389213]  [RRC reference
Culture Condition Recommended medium:
Luria broth supplemented with glucose (0.1%).
Other Remarks Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.