Gene Mutants - Detail

ME No. ME9141
Strain Name AQ705
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype metB1 dnaA46 his-29 trpA9605 pro thyA deoB/C
Parent Donor AQ401(EH116)
Parent Recipient AQ634
Methods TD./PI
Latest Marker Ts, dnaA46
Selection Ilv+
Source
Gene Mutants Detail Info
Reference Masai H, Asai T, Kubota Y, Arai K, Kogoma T.
Escherichia coli PriA protein is essential for inducible and constitutive stable DNA replication.
EMBO J  (1994)  13(22)   5338-45  
[PubMed ID = 7525276]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
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