Gene Mutants - Detail

ME No. ME9139
Strain Name AQ694
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 metB1 argH thyA lac-3 rpoB340 deoB (or deoC) proA3 rnhA224
Parent Donor AQ677
Parent Recipient AQ685
Methods TD./PI
Latest Marker cSDR+, rnhA224
Selection MetD+
Source
Gene Mutants Detail Info
Reference Kogoma T, von Meyenburg K.
The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.
EMBO J  (1983)  2(3)   463-8  
[PubMed ID = 11894964]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Casamino acids (0.2%), Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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