Gene Mutants - Detail

ME No. ME9138
Strain Name AQ685
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 metB1 argH thyA lac-3 rpoB340 deoB (or deoC) proA3 metD88
Parent Donor CD4(HfrC)
Parent Recipient AQ683
Methods CROSS
Latest Marker ilv+
Selection Ilv+
Source
Gene Mutants Detail Info
Reference Kogoma T, von Meyenburg K.
The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.
EMBO J  (1983)  2(3)   463-8  
[PubMed ID = 11894964]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
Request
page-top
/ecoli/strain