Gene Mutants - Detail

ME No. ME9136
Strain Name AQ666
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) rnhA224
Parent Donor
Parent Recipient
Methods
Latest Marker Ls
Selection
Source
Gene Mutants Detail Info
Reference Ogawa T, Pickett GG, Kogoma T, Kornberg A.
RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro.
Proc Natl Acad Sci U S A  (1984)  81(4)   1040-4  
[PubMed ID = 6322184]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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