Gene Mutants - Detail

ME No. ME9133
Strain Name AQ659
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thy lacY14 rha malB polA1 rpsL
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source from J. Wechsler
Gene Mutants Detail Info
Reference Subia NL, Kogoma T.
Concatemer formation of ColE1-type plasmids in mutants of Escherichia coli lacking RNase H activity.
J Mol Biol  (1986)  189(3)   389-99  
[PubMed ID = 3023625]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as JW164
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
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