Gene Mutants - Detail

ME No. ME9132
Strain Name AQ634
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 proB ilv metB1 thyA deoB (or deoC)
Parent Donor PC2
Parent Recipient AQ624
Methods TD./PI
Latest Marker Rif-s, rpoB+
Selection Arg+
Source
Gene Mutants Detail Info
Reference Mizukoshi T, Tanaka T, Arai K, Kohda D, Masai H.
A critical role of the 3' terminus of nascent DNA chains in recognition of stalled replication forks.
J Biol Chem  (2003)  278(43)   42234-9  
[PubMed ID = 12917421]  [RRC reference] 
Ogawa T, Pickett GG, Kogoma T, Kornberg A.
RNase H confers specificity in the dnaA-dependent initiation of replication at the unique origin of the Escherichia coli chromosome in vivo and in vitro.
Proc Natl Acad Sci U S A  (1984)  81(4)   1040-4  
[PubMed ID = 6322184]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
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