Gene Mutants - Detail

ME No. ME9131
Strain Name AQ624
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 proB (or proC) ilv metB1 argH thyA rpoB340 deoB (or deoC)
Parent Donor
Parent Recipient AQ614(LS534)
Methods SPONTANEOUS
Latest Marker thyA
Selection low thyimine requirement
Source
Gene Mutants Detail Info
Reference Kogoma T, von Meyenburg K.
The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.
EMBO J  (1983)  2(3)   463-8  
[PubMed ID = 11894964]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
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