Gene Mutants - Detail

ME No. ME9127
Strain Name AQ569
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype lexB30 thi-1 thr-1 leu-6 pyrF43 thyA6 deoC1 lacY1 tonA21 supE44 srlC::Tn10
Parent Donor AQ536(JC10240)
Parent Recipient AQ559(GY1163)
Methods TD./PI
Latest Marker srlC300::Tn10
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Torrey TA, Kogoma T.
Suppressor mutations (rin) that specifically suppress the recA+ dependence of stable DNA replication in Escherichia coliK-12.
Mol Gen Genet  (1982)  187(2)   225-30  
[PubMed ID = 6757658]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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