Gene Mutants - Detail

ME No. ME9124
Strain Name AQ548
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thy thi metB1 thiA lac rpsL dnaE293(Ts)
Parent Donor SL51
Parent Recipient AQ466
Methods CROSS
Latest Marker Ts, dnaE293(Ts)
Selection Leu+, Met-
Source from Connaughton
Gene Mutants Detail Info
Reference Cao Y, Kogoma T.
Requirement for the polymerization and 5'-->3' exonuclease activities of DNA polymerase I in initiation of DNA replication at oriK sites in the absence of RecA in Escherichia coli rnhA mutants.
J Bacteriol  (1993)  175(22)   7254-9  
[PubMed ID = 8226672]  [RRC reference] 
Culture Condition Recommended medium:
Luria broth supplemented with glucose (0.1%).
Other Remarks Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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