Gene Mutants - Detail

ME No. ME9123
Strain Name AQ547
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype metE90 trpA9605 trpR55 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA56 srlC300::Tn10 rin-15
Parent Donor JC10240
Parent Recipient AQ527
Methods TD./PI
Latest Marker UV-s, recA56
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Torrey TA, Kogoma T.
Suppressor mutations (rin) that specifically suppress the recA+ dependence of stable DNA replication in Escherichia coliK-12.
Mol Gen Genet  (1982)  187(2)   225-30  
[PubMed ID = 6757658]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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