Gene Mutants - Detail

ME No. ME9114
Strain Name AQ300
Sex Hfr
Plasmid Integrated F HfrC
Plasmid Autonomous
Genotype HfrC thyA42 metE101 arg-19 rnhA102 deoB20
Parent Donor HfrC(SL230)
Parent Recipient AQ145
Methods CROSS
Latest Marker HfrC
Selection Trp+, Pyr+
Reference Torrey TA, Kogoma T.
Suppressor mutations (rin) that specifically suppress the recA+ dependence of stable DNA replication in Escherichia coliK-12.
Mol Gen Genet  (1982)  187(2)   225-30  
[PubMed ID = 6757658]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks E. coli15T-
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.