Gene Mutants - Detail

ME No. ME9113
Strain Name AQ291
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype proB48 his-29 metE90 trpA9605 trpR55 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29
Parent Donor
Parent Recipient AQ271
Methods OTHER METHODS MUTAGENESIS/OthersTrimethoprim selection
Latest Marker Thy-, thyA708
Selection
Source
Gene Mutants Detail Info
Reference Torrey TA, Kogoma T.
Suppressor mutations (rin) that specifically suppress the recA+ dependence of stable DNA replication in Escherichia coliK-12.
Mol Gen Genet  (1982)  187(2)   225-30  
[PubMed ID = 6757658]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
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