Gene Mutants - Detail

ME No. ME9105
Strain Name AQ160
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype thr-1 leuB6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL31 tsx-33 sup-37 lexA3 tif-1 sfiA11 spr-51( lexA51 )
Parent Donor
Parent Recipient DM1180
Methods MUTAGENESIS/OthersMitomycin-C reisistant
Latest Marker
Selection MitomycinC resistant
Source from David Mount
Reference Kogoma T, Torrey TA, Connaughton MJ.
Induction of UV-resistant DNA replication in Escherichia coli: induced stable DNA replication as an SOS function.
Mol Gen Genet  (1979)  176(1)   1-9  
[PubMed ID = 396443]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as DM1187: Mount D. W., 1977, Proc. Natl. Acad. sci. USA, 74, 300-304.
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.