Gene Mutants - Detail

ME No. ME9103
Strain Name AQ157
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thr-1 leuB6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL31 tsx-33 sup-37 lexA3
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source from D.W.Mount
Reference Kogoma T, Hong X, Cadwell GW, Barnard KG, Asai T.
Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase HI and exonuclease V activities.
Biochimie  (1993)  75(1-2)   89-99  
[PubMed ID = 8389213]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as DM49: Mount D. W., 1977, Proc. Natl. Acad. sci. USA, 74, 300-304.
Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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