培養条件 - 詳細

Gene Symbol rna
Mnemonic RNaseA
Synonyms rnsA
Gene Product RNase I
Selectable Phenotype unknown
Culture Condition nutrient broth, Casamino acids or Casamino acids glucose medium (compositions described in Notes)
Growth Condition L broth
Teachers Comment
文献 Gessteland, R. F.
J. Mol. Biol. 1966. 16: 67-84
Other Phenotypes
Notes •nutrient broth containing per liter10 g of Bactotryptone (Difco), 1 g of yeast extract (Difco), 8 g of NaCl and 2 mM of CaCl2,
•Casamino acids glycerol medium containing per liter 5 g of Casamino acids (Difco), 5 g of NaCl, 1 g of NH4Cl, 4 ml of glycerol, 0.1 mM of Na2HPO4, 10 microM of FeCl3, 1 mM of MgSO4, 1.21 g of tris (Sigma 121) and 2 mM of CaCl2
•Casamino acids glucose medium containing per liter 2 g of Casamino acids (Difco, vitamine-free), 1 g of NaCl, 2 g of dextrose, 2 g of (NH4)2SO4, 20 ml of 1 M potassium phosphate buffer (pH 6.8) and 0.5 ml of salt solution containing 32 of MgCl2, 0.6 g of CaCl2 and 0.2 g of Fe2(SO4)3 in 100 ml
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