培養条件 - 詳細

Gene Symbol pfl
Mnemonic pyruvate formate-lyase
Synonyms
Gene Product
Selectable Phenotype give white colonies by overlaid soft agar (see Notes) (wild type: purple colonies)
Culture Condition rich broth containing (per liter) 10 g of tryptone, 5 g of NaCl, 1 g of yeast extract
Growth Condition L broth
Teachers Comment
文献 Chang, Y.-Y. & J. E. Cronan, Jr.
J. Bacteriol. 1982. 151: 1279-1289
Other Phenotypes
Notes •soft agar (5 ml) is containing 2.5 ml of 1.5 % agar, 1.37 ml of 10 % sodium pyruvate, 1 ml of 0.5 % benzyl viologen, and 0.125 ml of 1 M potassium phosphate buffer (pH 7.0)
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