培養条件 - 詳細

Gene Symbol nupG
Mnemonic nucleoside permease
Synonyms gru
Gene Product nucleoside permease
Selectable Phenotype sensitive to showdomycin (0.16 mM)
Culture Condition phosphate-buffered minimal medium containing 15 mM of (NH4)2SO4, 42 mM of Na2HPO4, 50 mM of NaCl, 22 mM of KH2PO4, 2 mM of MgCl2, 0.1 mM of CaCl2, and 0.003 mM of FeCl3 supplemented with glycerol (0.2 %)
Growth Condition L broth
Teachers Comment
文献 Munch-Petersen, A. & B. Mygind
J. Cell. Physiol. 1976. 89: 551-560
Other Phenotypes •unable to grow on guanine as sole carbon source
Notes •in nupC-: resistant to resistant to fluorouracil (2.5 μg/ml), fluorouridine (10 μg/ml), and fluorocytidine (10 μg/ml)
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