培養条件 - 詳細

Gene Symbol lacY
Mnemonic lactose
Synonyms
Gene Product galactoside permease (M protein)
Selectable Phenotype form white colonies on MacConkey lactose plates
Culture Condition DYT broth containing 1.6 % Bacto tryptone (Difco), 1.0 % yeast extract (Difco), and 0.5 % NaCl
Growth Condition L broth
Teachers Comment
文献 Hobson, A. C. et al.
J. Bacteriol. 1977. 131. 830-833
Other Phenotypes •form blue colonies on MacConkey-lactose plate containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (xgal, 0.04 g/li with 4 g/l of glucose as a carbon source) and ispropyl-thio-beta-D-galactoside (IPTG, 0.048 g/l) (WT: dark purple, lacZ-lacY-: white)
•resistant to o-nitrophenyl-beta-D-galactopyranoside (2 mM) (J. Bacteriol. 1973. 114: 882-884 )
Notes
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