Culture Condition - Detail

Gene Symbol adhE
Mnemonic alcohol/acetald.dehydrogenase
Synonyms ana adhC
Gene Product alcohol dehydrogenase, acetaldehyde dehydrogenase, CoA-linked
Selectable Phenotype resistant to allyl alcohol (100 mM) anaerobically
Culture Condition rich broth containing (per liter) tryptone (10 g), NaCl (5 g), and yeast extract (1 g)
Growth Condition L broth
Teachers Comment
Reference Lorowitz, W. & D. Clark
J. Bacteriol. 1982. 152: 935-938
Other Phenotypes •unable to grow aerobically in minimal media and sensitive to oxiditive stress (deletion mutant)(J. Biol. Chem. 2003. 278: 30193-30198)