このページではJavaScriptを使用しています。JavaScriptを有効にしてからご利用ください。

培養条件 - 詳細

Gene Symbol		ackA
Mnemonic		acetate kinase
Synonyms
Gene Product		acetate kinase
Selectable Phenotype		grow on media containing 25 mM pyruvate or proline as sole carbon sources despite the presence of 10 or 50 mM sodium fluoroacetate
Culture Condition		defined liquid media containing 50 mM-sodium/potassium phosphate buffer pH 7.2, 50 mM-ammonium chloride, essential salts (4 micro-g of CaCl₂.6H₂0; 8 mg of MgSO₄.7H₂O; 0.4 mig of MnSO₄.4H₂O; 0.4 mg FeSO₄.7H₂O per 100 ml of medium) and carbon source at 25 mM
Growth Condition		L broth
Teachers Comment
文献		Brown, T. D. K. et al. J. Gen. Microbiol. 1977. 102: 327-336
Other Phenotypes		•grow poorly on acetate (25 mM) as sole carbon source
Notes		•in ackA^- pta^-:grow poorly on high concentrations(more than 25 mM) (J. Bacteriol. 1995. 177: 2878-2886) •in acs^- ackA^- pta^-unable to grow on acetate at any concentration tested (J. Bacteriol. 1995. 177: 2878-2886)