Culture Condition - Detail

Gene Symbol ackA
Mnemonic acetate kinase
Synonyms
Gene Product acetate kinase
Selectable Phenotype grow on media containing 25 mM pyruvate or proline as sole carbon sources despite the presence of 10 or 50 mM sodium fluoroacetate
Culture Condition defined liquid media containing 50 mM-sodium/potassium phosphate buffer pH 7.2, 50 mM-ammonium chloride, essential salts (4 micro-g of CaCl2.6H20; 8 mg of MgSO4.7H2O; 0.4 mig of MnSO4.4H2O; 0.4 mg FeSO4.7H2O per 100 ml of medium) and carbon source at 25 mM
Growth Condition L broth
Teachers Comment
Reference Brown, T. D. K. et al.
J. Gen. Microbiol. 1977. 102: 327-336
Other Phenotypes •grow poorly on acetate (25 mM) as sole carbon source
Notes •in ackA- pta-:grow poorly on high concentrations(more than 25 mM) (J. Bacteriol. 1995. 177: 2878-2886)
•in acs- ackA- pta-unable to grow on acetate at any concentration tested (J. Bacteriol. 1995. 177: 2878-2886)
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