breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 1,387 C→T Q351* (CAA→TAA)  thrA → fused aspartate kinase/homoserine dehydrogenase 1
RA 11,648 G→A P47S (CCT→TCT)  yaaI ← DUF2541 domain‑containing protein YaaI
RA 42,234 G→A intergenic (‑303/‑169) caiT ← / → fixA L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA
RA 52,285 G→A A49V (GCG→GTG)  rsmA ← 16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase
RA 52,840 G→A R193W (CGG→TGG)  pdxA ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase
RA 69,155 G→A C298C (TGC→TGT araB ← ribulokinase
RA 76,954 G→A R116C (CGC→TGC)  sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 84,121 G→A intergenic (‑413/‑247) leuL ← / → leuO leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
RA 84,430 G→A M21I (ATG→ATA leuO → DNA‑binding transcriptional dual regulator LeuO
RA 88,015 G→A intergenic (+167/‑13) ilvH → / → cra acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit/DNA‑binding transcriptional dual regulator Cra
RA 94,528 G→A A455T (GCT→ACT)  murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 138,261 G→A M393I (ATG→ATA cueO → multicopper oxidase CueO
RA 140,131 G→A I365I (ATC→ATT gcd ← quinoprotein glucose dehydrogenase
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
RA 249,014 C→T intergenic (+880/‑1884) rayT → / → dinB REP‑associated tyrosine transposase/DNA polymerase IV
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 272,651 C→T noncoding (72/75 nt) eyeA → small RNA EyeA
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 289,676 G→A G210G (GGC→GGT argF ← ornithine carbamoyltransferase ArgF
RA 311,172 C→T E55E (GAG→GAA matA ← DNA‑binding transcriptional dual regulator MatA
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 317,170 A→G intergenic (+717/+556) insF1 → / ← rclC IS3 element protein InsF/DUF417 domain‑containing inner membrane protein RclC
RA 321,740 C→T P45S (CCT→TCT)  ykgE → putative lactate utilization oxidoreductase YkgE
RA 339,935 C→T A56V (GCC→GTC)  yahH → putative uncharacterized protein YahH
RA 339,941 C→T T58I (ACT→ATT)  yahH → putative uncharacterized protein YahH
RA 339,958 G→A D64N (GAC→AAC)  yahH → putative uncharacterized protein YahH
RA 354,894 G→A intergenic (+302/‑28) prpE → / → codB propionyl‑CoA synthetase/cytosine transporter
RA 412,670 G→A I1028I (ATC→ATT sbcC ← ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit
RA 430,773 G→A D57N (GAT→AAT)  yajD → HNH nuclease family protein YajD
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
MC JC 696,831 Δ109 bp [glnU] [glnU]
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
RA 761,007 G→T G768W (GGG→TGG)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 824,264 C→T W78* (TGG→TGA ybhP ← endonuclease/exonuclease/phosphatase domain‑containing protein YbhP
RA 827,789 C→T G398D (GGT→GAT)  ybhF ← ABC exporter ATP binding subunit YbhF
RA 938,553 G→A G187D (GGT→GAT)  rarA → replication‑associated recombination protein A
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,069,743 C→T A33T (GCA→ACA)  rutG ← pyrimidine:H(+) symporter
RA 1,098,620 T→A V245V (GTT→GTA ghrA → glyoxylate/hydroxypyruvate reductase A
RA 1,109,216 C→T intergenic (‑275/‑119) opgC ← / → opgG osmoregulated periplasmic glucans (OPG) biosynthesis protein C/osmoregulated periplasmic glucans biosynthesis protein G
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,162,923 G→A G96R (GGG→AGG)  lpoB → outer membrane lipoprotein ‑ activator of MrcB activity
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase
RA 1,216,559 C→T T64I (ACA→ATA)  ariR → putative two‑component system connector protein AriR
RA 1,255,799 G→A Q52* (CAA→TAA)  ycgV ← putative autotransporter adhesin YcgV
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,382,729 C→T Y261Y (TAC→TAT ompG → outer membrane porin G
RA 1,385,261 C→T A382V (GCG→GTG)  ycjX → DUF463 domain‑containing protein YcjX
RA 1,400,310 C→T M488I (ATG→ATA abgT ← p‑aminobenzoyl glutamate:H(+) symporter
RA 1,403,209 C→T R16H (CGC→CAC)  abgB ← p‑aminobenzoyl‑glutamate hydrolase subunit B
RA 1,422,412 C→T L144L (CTG→TTG)  ydaV → putative ATP‑binding protein YdaV
RA 1,439,093 C→T M564I (ATG→ATA ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,447,828 C→T L104L (CTG→TTG)  feaB → phenylacetaldehyde dehydrogenase
RA 1,464,904 G→A S145N (AGT→AAT)  paaY → 2‑hydroxycyclohepta‑1,4,6‑triene‑1‑carboxyl‑CoA thioesterase
RA 1,476,267 G→A W375* (TGG→TAG)  ydbD → DUF2773 domain‑containing protein YdbD
RA 1,476,872 C→T H577Y (CAC→TAC)  ydbD → DUF2773 domain‑containing protein YdbD
RA 1,478,864 C→T V213V (GTC→GTT ynbB → putative CDP‑diglyceride synthase
RA 1,482,714 C→T P49P (CCG→CCA azoR ← FMN dependent NADH:quinone oxidoreductase
RA 1,487,197 C→T intergenic (+234/‑38) hrpA → / → ydcF ATP‑dependent 3'‑>5' RNA helicase HrpA/DUF218 domain‑containing protein YdcF
RA 1,504,534 C→T S293L (TCA→TTA)  insQ → putative RNA‑guided DNA endonuclease InsQ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
RA 1,742,285 G→A L291L (CTG→CTA cfa → cyclopropane fatty acyl phospholipid synthase
RA 1,773,578 C→T P356L (CCA→CTA)  ydiN → putative transporter YdiN
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,964,254 A→C V266G (GTC→GGC)  flhA ← flagellar biosynthesis protein FlhA
RA 2,018,045 C→T Q34* (CAG→TAG)  fliJ → flagellar biosynthesis protein FliJ
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,143,828 C→T P188L (CCA→CTA)  dgcE → putative diguanylate cyclase DgcE
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,176,177 G→A Q49* (CAG→TAG)  gatZ ← putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
RA 2,254,795 G→A A131V (GCG→GTG)  rihB ← pyrimidine‑specific ribonucleoside hydrolase RihB
RA 2,281,282 G→A Q217Q (CAG→CAA radD → putative DNA repair helicase RadD
RA 2,335,464 G→A A394V (GCG→GTG)  yfaA ← DUF2138 domain‑containing protein YfaA
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,444,820 C→T A247A (GCG→GCA yfcA ← putative transporter YfcA
RA 2,447,079 G→A A132V (GCC→GTC)  aroC ← chorismate synthase
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,587,666 C→T T24T (ACC→ACT acrD → multidrug efflux pump RND permease AcrD
RA 2,724,285 G→A R53Q (CGG→CAG)  yfiM → DUF2279 domain‑containing protein YfiM
RA 2,729,586 G→A intergenic (‑142/+30) gltW ← / ← rrsG tRNA‑Glu/16S ribosomal RNA
RA 2,779,623 C→T S1035N (AGC→AAC)  ypjA ← adhesin‑like autotransporter YpjA
RA 2,838,241 G→A intergenic (‑136/+13) hydN ← / ← ascG putative electron transport protein HydN/DNA‑binding transcriptional repressor AscG
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 2,923,941 C→T R58Q (CGA→CAA)  yqcC ← DUF446 domain‑containing protein YqcC
RA 2,924,310 C→T noncoding (215/369 nt) csrB ← small regulatory RNA CsrB
RA 2,931,805 C→T R251W (CGG→TGG)  ygdG → flap endonuclease
RA 2,937,524 C→T I19I (ATC→ATT fucK → L‑fuculokinase
RA 2,951,111 C→T V451M (GTG→ATG)  recD ← exodeoxyribonuclease V subunit RecD
JC JC 2,955,094 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp coding (905‑910/3543 nt) recB ← exodeoxyribonuclease V subunit RecB
RA 2,959,564 C→T W955* (TGG→TGA recC ← exodeoxyribonuclease V subunit RecC
RA 2,988,926 C→T A142V (GCA→GTA)  yqeI → putative transcriptional regulator YqeI
RA 2,990,642 C→T intergenic (‑282/‑626) yqeK ← / → ygeG protein YqeK/TPR repeat‑containing putative chaperone YgeG
RA 3,057,584 G→A P276L (CCA→CTA)  serA ← phosphoglycerate dehydrogenase
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,136,616 C→T intergenic (‑245/+47) pitB ← / ← gss metal phosphate:H(+) symporter PitB/fused glutathionylspermidine amidase/glutathionylspermidine synthetase
RA 3,213,461 G→A E139K (GAA→AAA)  rpoD → RNA polymerase sigma factor RpoD
RA 3,219,727 C→T D78D (GAC→GAT patA → putrescine aminotransferase
RA 3,231,198 C→T H771Y (CAT→TAT)  ygjK → glycoside hydrolase
RA 3,234,081 C→T A22T (GCG→ACG)  higA ← antitoxin/DNA‑binding transcriptional repressor HigA
RA 3,261,415 T→G D335A (GAC→GCC)  tdcE ← 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
RA 3,271,554 C→T V73M (GTG→ATG)  garK ← glycerate 2‑kinase 1
RA 3,311,284 C→T noncoding (102/216 nt) sraG → small regulatory RNA SraG
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,445,166 A→G S22P (TCT→CCT)  rpsE ← 30S ribosomal subunit protein S5
RA 3,464,134 C→T A71A (GCC→GCT gspL → Type II secretion system protein GspL
RA 3,472,093 C→T K474K (AAG→AAA fusA ← elongation factor G
RA 3,492,429 C→T intergenic (‑132/‑139) ppiA ← / → tsgA peptidyl‑prolyl cis‑trans isomerase A/putative transporter TsgA
JC 3,499,823 Δ5 bp intergenic (+208/‑83) yhfL → / → frlA DUF4223 domain‑containing lipoprotein YhfL/fructoselysine/psicoselysine transporter
RA 3,502,008 C→T F247F (TTC→TTT frlB → fructoselysine 6‑phosphate deglycase
RA 3,503,153 C→T P272S (CCG→TCG)  frlC → fructoselysine 3‑epimerase
RA 3,504,390 C→T A113A (GCC→GCT frlR → DNA‑binding transcriptional regulator FrlR
RA 3,504,700 C→T Q217* (CAG→TAG)  frlR → DNA‑binding transcriptional regulator FrlR
RA 3,515,404 C→T A170A (GCG→GCA dam ← DNA adenine methyltransferase
RA 3,516,543 C→T R255H (CGC→CAC)  damX ← cell division protein DamX
RA 3,516,814 C→T E165K (GAA→AAA)  damX ← cell division protein DamX
RA 3,542,195 A→G I597V (ATT→GTT)  feoB → Fe(2(+)) transporter FeoB
RA 3,542,270 C→T Q622* (CAG→TAG)  feoB → Fe(2(+)) transporter FeoB
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,563,542 G→A intergenic (+24/+182) glpD → / ← yzgL aerobic glycerol 3‑phosphate dehydrogenase/putative uncharacterized protein YzgL
RA 3,622,220 G→A R1010Q (CGG→CAG)  rhsB → rhs element protein RhsB
RA 3,643,244 G→A L647L (CTG→TTG)  prlC ← oligopeptidase A
RA 3,651,471 G→A pseudogene (181/746 nt) yhiS → putative uncharacterized protein YhiS
RA 3,682,515 C→T M311I (ATG→ATA dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,693,807 G→A P477L (CCG→CTG)  bcsA ← cellulose synthase catalytic subunit
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,743,273 G→A G181S (GGC→AGC)  yiaK → 2,3‑diketo‑L‑gulonate reductase
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,774,117 G→A D613N (GAT→AAT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,778,054 G→A G219D (GGT→GAT)  lldP → lactate/glycolate:H(+) symporter LldP
RA 3,815,589 G→A S60S (TCC→TCT pyrE ← orotate phosphoribosyltransferase
RA 3,815,689 G→A S27F (TCC→TTC)  pyrE ← orotate phosphoribosyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,849,801 C→T L112L (TTG→TTA uhpB ← sensory histidine kinase UhpB
RA 3,850,840 G→A P85S (CCG→TCG)  ilvN ← acetohydroxy acid synthase I subunit IlvN
RA 3,861,090 G→A A270T (GCC→ACC)  yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,870,496 G→A A412V (GCG→GTG)  dgoT ← D‑galactonate:H(+) symporter
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,891,585 C→T intergenic (+102/‑30) tnaB → / → mdtL tryptophan:H(+) symporter TnaB/efflux pump MdtL
RA 3,901,242 C→T D327N (GAT→AAT)  bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,912,829 G→A S280S (AGC→AGT glmS ← L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
RA 3,919,729 G→A G43G (GGC→GGT atpA ← ATP synthase F1 complex subunit alpha
RA 3,943,881 G→A noncoding (178/2904 nt) rrlC → 23S ribosomal RNA
RA 3,944,127 G→A noncoding (424/2904 nt) rrlC → 23S ribosomal RNA
RA 3,948,492 G→A A493V (GCG→GTG)  yifB ← putative magnesium chelatase YifB
RA 3,957,234 G→A A196V (GCC→GTC)  ilvY ← DNA‑binding transcriptional dual regulator IlvY
RA 3,963,294 C→T T312T (ACG→ACA gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,975,797 C→T R218C (CGT→TGT)  wecE → dTDP‑4‑dehydro‑6‑deoxy‑D‑glucose transaminase
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA
RA 3,997,726 G→A E182K (GAG→AAG)  yigB → 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
RA 4,011,802 C→T P243S (CCG→TCG)  bioP → biotin transporter
RA 4,016,421 G→A intergenic (‑252/‑10) ysgA ← / → udp putative dienelactone hydrolase/uridine phosphorylase
RA 4,038,486 C→T noncoding (968/2905 nt) rrlA → 23S ribosomal RNA
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,095,424 G→A P187S (CCG→TCG)  rhaA ← L‑rhamnose isomerase
RA 4,110,568 G→A A213A (GCG→GCA cdh → CDP‑diacylglycerol diphosphatase
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,115,681 G→A intergenic (‑102/+33) glpX ← / ← glpK fructose‑1,6‑bisphosphatase 2/glycerol kinase
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR
RA 4,164,123 C→G A162G (GCA→GGA)  btuB → cobalamin outer membrane transporter
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,194,432 G→A A556A (GCC→GCT thiC ← phosphomethylpyrimidine synthase
RA 4,196,331 C→G intergenic (‑232/+1) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,282,332 (A)5→6 coding (744/1293 nt) yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,308,783 G→A I229I (ATC→ATT alsC ← D‑allose ABC transporter membrane subunit
RA 4,312,125 G→A F289F (TTC→TTT alsR ← DNA‑binding transcriptional repressor AlsR
RA 4,318,717 C→T A16A (GCG→GCA phnK ← carbon‑phosphorus lyase subunit PhnK
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,300 C→T L267L (CTG→TTG)  proP → osmolyte:H(+) symporter ProP
RA 4,339,307 C→T A405A (GCG→GCA adiA ← arginine decarboxylase, degradative
RA 4,345,284 C→T W92* (TGG→TAG)  yjdF ← DUF2238 domain‑containing inner membrane protein YjdF
RA 4,357,810 C→T G270S (GGT→AGT)  cadA ← lysine decarboxylase 1
RA 4,368,347 C→T intergenic (‑20/‑317) aspA ← / → fxsA aspartate ammonia‑lyase/protein FxsA
RA 4,375,493 C→T V55V (GTG→GTA epmB ← lysine 2,3‑aminomutase
RA 4,377,568 C→T R52H (CGC→CAC)  blc ← outer membrane lipoprotein Blc
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,394,251 C→T H62H (CAC→CAT nnr → NAD(P)HX epimerase/NAD(P)HX dehydratase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,439,165 C→T G33D (GGC→GAC)  ytfJ ← PF09695 family protein YtfJ
RA 4,441,587 C→T G197D (GGT→GAT)  msrA ← methionine sulfoxide reductase A
RA 4,452,269 C→T S404S (TCC→TCT ytfR → galactofuranose ABC transporter putative ATP binding subunit
RA 4,459,323 C→T intergenic (+12/+577) tldE → / ← nrdG metalloprotease subunit TldE/anaerobic ribonucleoside‑triphosphate reductase activating protein
RA 4,487,727 C→T A137V (GCG→GTG)  lptG → lipopolysaccharide transport system protein LptG
RA 4,488,658 C→T G469D (GGT→GAT)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,495,234 C→T D326N (GAC→AAC)  ahr ← NADPH‑dependent aldehyde reductase Ahr
RA 4,495,531 C→T A227T (GCA→ACA)  ahr ← NADPH‑dependent aldehyde reductase Ahr
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,523,680 C→T A139T (GCA→ACA)  yjhG ← D‑xylonate dehydratase
RA 4,523,712 C→T G128E (GGG→GAG)  yjhG ← D‑xylonate dehydratase
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196325 1211442 15118 39 [33] [38] 39 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270–1978503 9–776 151 [0] [0] 160 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423824–3424234 3424477–3424239 6–654 39 [38] [38] 39 [rrlD] [rrlD]
* * ÷ NC_000913 3708467 3708621 155 52 [0] [32] 41 [proK] [proK]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)152 (1.240) 86/582 0.1 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)152 (1.220) 87/590 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)160 (1.290) 94/590 0.0 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 = 37084660 (0.000)52 (0.660) 31/374 1.0 100% intergenic (‑761/+150) dppA/proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
?NC_000913 3708635 = 0 (0.000)noncoding (58/77 nt) proK tRNA‑Pro