breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 64 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,5220GT78.6% 23.3 / 14.4 28intergenic (+129/+586)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_0009132,304,5370AG76.7% 26.6 / 15.0 30intergenic (+144/+571)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_0009132,304,5230GC75.0% 24.0 / 19.2 29intergenic (+130/+585)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_0009132,304,5400CT68.8% 13.6 / 22.3 32intergenic (+147/+568)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_000913339,9860GA58.3% 19.7 / 40.7 36R73Q (CGA→CAA) yahHputative uncharacterized protein YahH
*NC_0009132,304,5490CT55.6% ‑5.2 / 41.4 37intergenic (+156/+559)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_000913339,9790GA52.8% 10.1 / 45.5 36V71M (GTG→ATG) yahHputative uncharacterized protein YahH
*NC_0009132,304,5670CT52.6% 5.5 / 48.4 38intergenic (+174/+541)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_0009132,304,5750GA51.3% 11.4 / 50.8 38intergenic (+182/+533)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_000913339,9920C.38.9% 65.4 / 59.6 36coding (224/321 nt)yahHputative uncharacterized protein YahH
*NC_00091358,0650CA38.5% 143.5 / 36.8 117P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009133,911,5330CA34.6% 288.5 / 43.3 185intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_000913799,4840CA33.7% 148.8 / 10.5 92S300* (TCG→TAG) pgl6‑phosphogluconolactonase
*NC_0009131,823,0180CA32.0% 179.8 / 11.8 122L187L (CTC→CTAnadENH3‑dependent NAD(+) synthetase
*NC_0009132,767,4440CA30.5% 311.7 / 11.1 132intergenic (+89/‑266)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_000913866,3190CA30.3% 188.5 / 13.8 123E16* (GAG→TAG) moeAmolybdopterin molybdotransferase
*NC_000913847,9550GA29.8% 252.5 / 67.8 188A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009132,722,8380GT29.7% 280.1 / 15.5 148E38* (GAG→TAG) pssAphosphatidylserine synthase
*NC_0009132,472,8170CA29.7% 237.8 / 15.2 119G22V (GGA→GTA) yfdNuncharacterized protein YfdN
*NC_00091342,3950GT29.6% 224.2 / 11.2 142intergenic (‑464/‑8)caiT/fixAL‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA

Marginal new junction evidence (lowest skew 10 of 54 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3133052 =101 (0.810)39 (0.320) 25/576 3.2 26.3% coding (161/759 nt) yghR putative ATP‑binding protein YghR
?NC_000913 = 3306041 120 (0.990)coding (1820/1890 nt) deaD ATP‑dependent RNA helicase DeaD
* ? NC_000913 4542682 =213 (1.720)28 (0.230) 22/572 3.6 14.0% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 139 (1.160)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 4542690225 (1.810)25 (0.210) 20/572 4.0 12.3% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 138 (1.150)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 3648146156 (1.260)13 (0.130) 13/474 4.6 8.9% intergenic (+2/‑148) agrA/agrB small RNA AgrA/small regulatory RNA AgrB
?NC_000913 3648378 = 141 (1.410)intergenic (+3/‑150) agrB/arsR small regulatory RNA AgrB/DNA‑binding transcriptional repressor ArsR
* ? NC_000913 = 4606166109 (0.880)11 (0.130) 8/412 5.5 13.2% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 68 (0.790)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 294746797 (0.780)9 (0.100) 8/424 5.6 11.8% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 65 (0.730)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 = 2653419122 (0.980)13 (0.110) 11/540 5.8 9.7% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 130 (1.140)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 780918102 (0.820)9 (0.100) 8/438 5.8 10.8% noncoding (76/76 nt) lysW tRNA‑Lys
?NC_000913 781445 = 73 (0.790)intergenic (+1/‑132) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
* ? NC_000913 = 2357730153 (1.230)7 (0.070) 7/510 7.0 5.2% coding (827/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357926 = 124 (1.160)intergenic (+123/+116) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 781577 =81 (0.650)8 (0.070)
+GAAG
8/582 7.2 7.3% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 125 (1.010)noncoding (76/76 nt) valU tRNA‑Val