Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,464,904	G→A	S145N (AGT→AAT)	paaY →	2‑hydroxycyclohepta‑1,4,6‑triene‑1‑carboxyl‑CoA thioesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,464,904	0	G	A	100.0%	232.7 / NA	72	S145N (AGT→AAT)	paaY	2‑hydroxycyclohepta‑1,4,6‑triene‑1‑carboxyl‑CoA thioesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/27);  total (45/27)

GTCGTATCGTGGTGAAAGATGGCGCGAACATTCAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGCTCTGCTTTATTTATTTGAGTATTAATTCATACCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAAT  >  NC_000913/1464613‑1465199                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                         gTCGTATCGTGGTGAAAGATGGCGCGAACATTCAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCANCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCa                                                                                                                                                                                                                                                                                                    >  2:801210/1‑298 (MQ=255)       tCGTGGTGAAAGATGGCGCAAACATTCAGGATAATTTCGTTATGCACGGTTTTCCCGAGCAGGATATTGTCGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGc                                                                                                                                                                                                                                                                                                     <  2:233823/291‑1 (MQ=255)            gtgAAAGATGGCGCGAACATTCAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTAGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCAt                                                                                                                                                                                                                                                                                         >  2:428789/1‑298 (MQ=255)                     ggCGCAAACATTCAGGATAATTGCCTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGc                                                                                                                                                                                                                                                                                <  2:232737/298‑1 (MQ=255)                              aTTCAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGTAGAAGATGGACATATTGTTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCa                                                                                                                                                                                                                                                                      <  1:82263/299‑1 (MQ=255)                                  aGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTNTCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAg                                                                                                                                                                                                                                                                  <  1:740778/299‑1 (MQ=255)                                   ggATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTa                                                                                                                                                                                                                                                                >  1:421096/1‑300 (MQ=255)                                    gATAATTGCGTTATGCATGGTTCTCCCGAGCAGGATACTGTTGTAGGAGAAGAGGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCAGTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGAGGGACGGTGCGGTGAGTGGCGAGAACAGCATTGTTGGTACATCCGCATTTGTGAAAGCCAAAGTAGAAATGCTAGCTAATTACCTGATTGTCGGCAACCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAggac                                                                                                                                                                                                                                                               <  2:117155/300‑4 (MQ=255)                                    gATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTAc                                                                                                                                                                                                                                                               <  1:792188/300‑1 (MQ=255)                                         ttGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCANGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGt                                                                                                                                                                                                                                                          <  1:198636/300‑1 (MQ=255)                                         ttGCGTTATGCAAGGTTTTCCCGAGCAGGATACTGTTTTAGTAGAAGATGGACATATTGTTCATAGCGCTATCCTTCACGGCTGCATTAGCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGTAGGGTACGCATGAGTACCAGGt                                                                                                                                                                                                                                                          <  2:27058/300‑1 (MQ=255)                                                aTGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTg                                                                                                                                                                                                                                                   <  1:925954/300‑1 (MQ=255)                                                               gAGCAGGATACTGTTGTAGTAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAAGGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGc                                                                                                                                                                                                                                     <  2:149845/298‑1 (MQ=255)                                                               gAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGc                                                                                                                                                                                                                                     <  1:399934/298‑1 (MQ=255)                                                                    ggATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGtt                                                                                                                                                                                                                              >  1:138488/1‑300 (MQ=255)                                                                    ggATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGACTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTCCGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAGGCAGACGtt                                                                                                                                                                                                                              >  1:134827/1‑300 (MQ=255)                                                                         cTGTTGTAGGCGAAGATGGACATATTGGTCATAGCGCTATCCTTCAAGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCAAAAGCAGAAATGCCAGCTAATTACCTTATTGTAGGCAGCCCAGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTCCCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATc                                                                                                                                                                                                                         >  2:283657/1‑300 (MQ=255)                                                                           gttgtAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCaa                                                                                                                                                                                                                       >  1:604346/1‑300 (MQ=255)                                                                             tgtAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGt                                                                                                                                                                                                                     <  1:281073/300‑1 (MQ=255)                                                                                              atatTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGaaa                                                                                                                                                                                                    >  1:370724/1‑300 (MQ=255)                                                                                                          gcgcTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCa                                                                                                                                                                                        >  2:636987/1‑300 (MQ=255)                                                                                                          gcgcTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTAAATCAAGTCGAGCAATTGCGGGAAATTGAACCTGGc                                                                                                                                                                                         >  2:444036/1‑299 (MQ=255)                                                                                                               aTCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGaaa                                                                                                                                                                                   <  1:801210/300‑1 (MQ=255)                                                                                                                 ccTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAAc                                                                                                                                                                                  >  1:606055/1‑299 (MQ=255)                                                                                                                       cGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGt                                                                                                                                                                           >  1:892530/1‑300 (MQ=255)                                                                                                                         gCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTAt                                                                                                                                                                         <  1:926280/300‑1 (MQ=255)                                                                                                                         gCTGCATTATCCGCCGCAAGGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTgggat                                                                                                                                                                         <  1:926382/300‑4 (MQ=255)                                                                                                                                                  aGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACa                                                                                                                                                >  1:805119/1‑300 (MQ=255)                                                                                                                                                  aGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCATGGTATTTGATGAGAATCTGCGACCGAAACa                                                                                                                                                >  1:805383/1‑300 (MQ=255)                                                                                                                                                      ggAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTaa                                                                                                                                            <  1:122669/300‑1 (MQ=255)                                                                                                                                                         aTGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAg                                                                                                                                         <  2:32153/300‑1 (MQ=255)                                                                                                                                                                  gTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGACCTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAat                                                                                                                                >  2:29421/1‑300 (MQ=255)                                                                                                                                                                  gTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAat                                                                                                                                >  2:26363/1‑300 (MQ=255)                                                                                                                                                                  gTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTAGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAat                                                                                                                                >  2:659469/1‑300 (MQ=255)                                                                                                                                                                     gTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGACAGCCAAAGCAGAAATACCAGCTCATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGACCTAAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATAAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACCTCAAGTCGCGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGTTATTTTCTGCGAATCCGCGACCGCCACCGTAACAGATGTAAActtat                                                                                                                             >  1:946276/1‑300 (MQ=255)                                                                                                                                                                     gTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACTTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAattat                                                                                                                             >  1:25805/1‑300 (MQ=255)                                                                                                                                                                     gTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAACCGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAattat                                                                                                                             >  2:177830/1‑300 (MQ=255)                                                                                                                                                                     gTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAattat                                                                                                                             >  1:29751/1‑300 (MQ=255)                                                                                                                                                                     gTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAattat                                                                                                                             >  2:177816/1‑300 (MQ=255)                                                                                                                                                                     gTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCATGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAattat                                                                                                                             >  1:30161/1‑300 (MQ=255)                                                                                                                                                                     gTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑AAACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAAAGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAattat                                                                                                                             >  1:986706/1‑300 (MQ=255)                                                                                                                                                                       gATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTAttt                                                                                                                           >  1:22775/1‑300 (MQ=255)                                                                                                                                                                       gATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCAATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTAttt                                                                                                                           >  1:21870/1‑300 (MQ=255)                                                                                                                                                                            aCGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAGTTATTTTGTcc                                                                                                                      >  1:307628/1‑300 (MQ=255)                                                                                                                                                                               gtgcggtgATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACCTCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCtt                                                                                                                   >  1:123457/1‑300 (MQ=255)                                                                                                                                                                               gtgcggtgATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTTAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTccatt                                                                                                                   >  1:923112/1‑297 (MQ=255)                                                                                                                                                                               gtgcggtgATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCtt                                                                                                                   >  1:672606/1‑300 (MQ=255)                                                                                                                                                                               gtgcggtgATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCtt                                                                                                                   >  2:615938/1‑300 (MQ=255)                                                                                                                                                                               gtgcggtgATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCACtt                                                                                                                   >  2:122499/1‑300 (MQ=255)                                                                                                                                                                               gtgcggtgATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTAAATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAAAAGATGTAAAAGTACTATGTCCCtt                                                                                                                   >  2:313358/1‑300 (MQ=255)                                                                                                                                                                                    gtgATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAAtt                                                                                                              <  2:202304/300‑1 (MQ=255)                                                                                                                                                                                      gATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTCTTTTGTCCCTTTAATtat                                                                                                            >  2:39103/1‑300 (MQ=255)                                                                                                                                                                                               gaACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCaga                                                                                                    >  2:599332/1‑299 (MQ=255)                                                                                                                                                                                                    tccctgttgGTACATCCGCATTTGTGAAAACCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCAGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGCAAACGCCGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTATAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGCACGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTa                                                                                                >  1:907173/5‑298 (MQ=255)                                                                                                                                                                                                      aTTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTTAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGt                                                                                             <  2:923112/299‑1 (MQ=255)                                                                                                                                                                                                       ttgttgGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGttt                                                                                           <  2:370724/300‑1 (MQ=255)                                                                                                                                                                                                       ttgttgGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCATTTAATTATAAAGCAGAGTTATGttt                                                                                           >  2:180640/1‑300 (MQ=255)                                                                                                                                                                                                         gttgGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTaa                                                                                         >  1:22969/1‑300 (MQ=255)                                                                                                                                                                                                           tgGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGc                                                                                       <  2:973632/300‑1 (MQ=255)                                                                                                                                                                                                           tgGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGc                                                                                       <  1:608945/300‑1 (MQ=255)                                                                                                                                                                                                           tgGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATGTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGc                                                                                       <  1:608988/300‑1 (MQ=255)                                                                                                                                                                                                             gTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGctc                                                                                     <  1:846816/300‑1 (MQ=255)                                                                                                                                                                                                                                      aGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGCTCTGCTTTATTTATTTGAGTATTAAtt                                                            <  2:672606/300‑1 (MQ=255)                                                                                                                                                                                                                                                    cTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGNGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTCTAAAGCAGAGTTATGTTTAAGCTCTGCTTTATTTATTTGAGTATTAATTCATACCGTTTTTtc                                              >  2:741131/1‑300 (MQ=255)                                                                                                                                                                                                                                                          aCCTAATTGTCGGCAGCCCGGCGAAAGCGATTCGTGCACTCAATGAACAGGCGTTGGCATGGAAAAAGCAGGGTACGCATGGGTACCAGGTGCTGGTG‑AAACGCTGTAAGTAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGAAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGCCCCTTTAATTATAAAGGAGAGTTATGTTTAAGCTTTGCTTTCTTTATTTGAGTATTAATTCATACCGTTTTTTCATCata                                        >  2:310491/1‑300 (MQ=255)                                                                                                                                                                                                                                                                ttGTCTGCAGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCATTGGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGCGCTG‑TGTACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTAGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGCTCTGCTTTATTTATTTGAGTATTAATTCATACCGTTCTTTCATCATATAatta                                  <  2:105077/300‑1 (MQ=255)                                                                                                                                                                                                                                                                ttGTCTGCAGCCCGGAGAAAGCGACTCGTAAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCATGCGCTGGTG‑ACACGCGGTAAGCATACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGAGGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTAGAAAGCAGAGTTATGTGTAAGCTCTGCTTTATTTATTTGAGTATGAATGCATACCGTTTTTTCATCATATAatga                                  <  2:106135/300‑3 (MQ=255)                                                                                                                                                                                                                                                                        aGCCCGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGCTCTGCTTTATTTATTTGAGTATTAATTCATACCGTTTTTTCATCATATAATTATTTATAAt                          >  2:716664/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            cGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGCTCTGCTTTATTTATTTGAGTATTAATTCACACCGTTTTTTCATCATATAATTATTTATCATgaga                      >  2:370036/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            cGGCGAAAGCGATTCGTGAACTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCATTTAATTATAAAGCAGAGTTATGTTTAAGCTCTGCTTTATTTATTTGAGTATTACTTCATACCGTTTTTTCATCATATAATTATTTATCATgaga                      >  2:371009/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                cTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGCTCTGCTTTATTTATTTGAGTATTAATTCATACCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAAt  >  2:575520/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                cTCAATGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCATTTAATTATAAAGCAGAGTTATGTTTAAGCTCTGCTTTATTTATTTGAGTATTAATTCATACCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTaa   >  2:561710/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                         GTCGTATCGTGGTGAAAGATGGCGCGAACATTCAGGATAATTGCGTTATGCACGGTTTTCCCGAGCAGGATACTGTTGTAGGAGAAGATGGACATATTGGTCATAGCGCTATCCTTCACGGCTGCATTATCCGCCGCAATGCATTAGTGGGAATGAACGCGGTAGTGATGGACGGTGCGGTGATTGGCGAGAACAGCATTGTTGGTGCATCCGCATTTGTGAAAGCCAAAGCAGAAATGCCAGCTAATTACCTGATTGTCGGCAGCCCGGCGAAAGCGATTCGTGAACTCAGTGAGCAGGAGTTGGCATGGAAAAAGCAGGGTACGCATGAGTACCAGGTGCTGGTG‑ACACGCTGTAAGCAGACGTTACATCAAGTCGAGCCATTGCGGGAAATTGAACCTGGCAGGAAACGCCTGGTATTTGATGAGAATCTGCGACCGAAACAGTAACAGATGTAAAATTATTTTGTCCCTTTAATTATAAAGCAGAGTTATGTTTAAGCTCTGCTTTATTTATTTGAGTATTAATTCATACCGTTTTTTCATCATATAATTATTTATAATGAGAATGTGGTTTTAATTTGTAAT  >  NC_000913/1464613‑1465199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑