breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,878,9470CT40.0% 25.4 / 12.8 30E276K (GAA→AAA) cas1multifunctional nuclease Cas1
*NC_0009133,548,9250AC32.1% 73.2 / 11.1 57G382G (GGT→GGGmalQ4‑alpha‑glucanotransferase
*NC_0009132,667,1260AC31.8% 97.9 / 10.8 66Y241D (TAT→GAT) hcaTputative 3‑phenylpropionate transporter
*NC_0009131,061,9300GA31.0% 88.3 / 19.1 58L44L (CTG→CTAtorDtrimethylamine‑N‑oxide reductase‑specific chaperone
*NC_000913613,2830AG27.2% 125.8 / 18.3 84I183V (ATT→GTT) fesferric enterobactin esterase
*NC_0009131,715,6200AC26.9% 77.6 / 10.9 52G90G (GGT→GGGpdxYpyridoxal kinase 2
*NC_000913300,1200CG26.8% 94.6 / 12.7 57R272P (CGC→CCC) paoCaldehyde dehydrogenase: molybdenum cofactor‑binding subunit
*NC_0009132,950,1730AG24.5% 101.0 / 11.0 53E311G (GAG→GGG) argAN‑acetylglutamate synthase
*NC_0009133,393,6790AT24.0% 96.7 / 10.2 50S860R (AGT→AGAyhdPphospholipid transport factor YhdP
*NC_000913566,3440AG23.1% 112.7 / 31.8 66intergenic (‑366/‑340)intD/renDputative integrase/protein RenD
*NC_000913566,2750AG21.7% 131.5 / 33.1 70intergenic (‑297/‑409)intD/renDputative integrase/protein RenD
*NC_000913566,2830GC21.5% 152.5 / 44.7 67intergenic (‑305/‑401)intD/renDputative integrase/protein RenD
*NC_000913566,3200AC21.5% 131.0 / 28.1 66intergenic (‑342/‑364)intD/renDputative integrase/protein RenD
*NC_000913640,8800TG21.5% 271.7 / 11.2 122G376G (GGT→GGGahpFalkyl hydroperoxide reductase, AhpF component
*NC_0009133,914,5230CT21.4% 138.5 / 11.8 71V226V (GTG→GTAglmUfused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase
*NC_000913566,2840CG21.2% 158.6 / 45.8 67intergenic (‑306/‑400)intD/renDputative integrase/protein RenD
*NC_000913566,3500CG21.2% 155.9 / 39.2 67intergenic (‑372/‑334)intD/renDputative integrase/protein RenD
*NC_000913566,1900CA21.0% 174.0 / 32.9 81intergenic (‑212/‑494)intD/renDputative integrase/protein RenD
*NC_000913566,3320TG20.9% 123.9 / 44.2 68intergenic (‑354/‑352)intD/renDputative integrase/protein RenD
*NC_000913566,3470GA20.9% 134.3 / 28.9 67intergenic (‑369/‑337)intD/renDputative integrase/protein RenD

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 345129568 (1.190)3 (0.050) 3/470 4.5 3.8% coding (70/822 nt) rplB 50S ribosomal subunit protein L2
?NC_000913 3451798 = 88 (1.610)coding (489/606 nt) rplD 50S ribosomal subunit protein L4
* ? NC_000913 = 2290113NA (NA)3 (0.050) 3/490 4.6 10.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 2904758 = 25 (0.440)coding (661/672 nt) queE putative 7‑carboxy‑7‑deazaguanine synthase QueE