Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	566,275	0	A	G	21.7%	131.5 / 33.1	70	intergenic (‑297/‑409)	intD/renD	putative integrase/protein RenD
Reads supporting (aligned to +/- strand):  ref base A (24/30);  new base G (10/5);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCGGAGACTGAAGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACG  >  NC_000913/566026‑566509                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           cAGCGGAGACTGAAGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCAt                                                                                                                                                                                                                                           >  1:313570/1‑251 (MQ=255)          cagAAGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGAACCCGGATATTATCGTGAGGATGCTTCATCGCCATTGCTCCCAAAATACGAAACCAATTTCAGCCATGGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTAGACAAAACACG‑AAATGTACTTTTCATTCCGCTCAATCACGACTTGATGCAGGCCTTCGCTCTCCCTGCGCGGGGCATAGTTAGCCAAGTACAAGTAATCTTTTcgcg                                                                                                                                                                                                                                  >  2:498403/3‑251 (MQ=255)                cgccgcAATGCAGGCTTGCCGTGTTGTGCGGTACCCGGATATTATCGTGAGGCTGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCCTCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGCCATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCATAGTACCAGGCATCTTTTcgcgaccccc                                                                                                                                                                                                                            >  2:387246/1‑245 (MQ=255)                         gCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGt                                                                                                                                                                                                                   <  2:194199/251‑1 (MQ=255)                             gCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTg                                                                                                                                                                                                               <  1:396227/251‑1 (MQ=255)                                    gtgtTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTGTTCGCGTCACCCACATGCTGTACTGCACCTgg                                                                                                                                                                                                        <  1:492051/251‑1 (MQ=255)                                     tgttgtGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACCTGCTGTACTGCACCTggg                                                                                                                                                                                                       >  2:547201/1‑251 (MQ=255)                                          tgAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATg                                                                                                                                                                                                  >  2:151949/1‑251 (MQ=255)                                            aGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTa                                                                                                                                                                                                <  2:169600/251‑1 (MQ=255)                                            aGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAACCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCCTAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGt                                                                                                                                                                                                 >  2:312234/1‑250 (MQ=255)                                            aGGTACACGGATATTATCGTGAGGATGCGTCATCGCCGTTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTACGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCCCCTGGGCCATGt                                                                                                                                                                                                 >  2:98621/1‑250 (MQ=255)                                             ggTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTaa                                                                                                                                                                                               <  1:358946/251‑1 (MQ=255)                                                 ccaggATATTATCGTGAGGCTGCGTCATCGCCATTGCTCCCCACATACAACACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCCCCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCCCCTGCTGTCCTGCCCCTGGGCCCTGTAGGCCg                                                                                                                                                                                           >  2:382817/4‑251 (MQ=255)                                                     gATAGTATCGGGAGGCAGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCGGCCAGTTCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATCTACTTTTCATTCAGCTCAATCACGACATAATGCAGGGCTTCACGCTTCATGACCGGGACATAGTTGGCAAAGTACCAGGCCTCTTTTCGCGTCACCCACATGCTGTACTGACCCTCGGCCATGTAAGCCgctat                                                                                                                                                                                       >  2:35829/1‑247 (MQ=255)                                                           atCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTGTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTg                                                                                                                                                                                  <  2:153907/250‑1 (MQ=255)                                                              gTGATGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGGGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTc                                                                                                                                                                              <  2:465636/251‑1 (MQ=255)                                                              gTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTc                                                                                                                                                                              <  1:484961/251‑1 (MQ=255)                                                                       cGTCAACGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAaccacc                                                                                                                                                                     <  1:153782/251‑1 (MQ=255)                                                                                   tGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAAtt                                                                                                                                                          <  1:519864/250‑1 (MQ=255)                                                                                     ttccccgaacgtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTc                                                                                                                                                        >  1:537628/26‑250 (MQ=255)                                                                                       ccccgaacgtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCAt                                                                                                                                                      <  1:274275/227‑1 (MQ=255)                                                                                       ccccAAATACAAAACCAATTCCAGCCAGTGCCTCGTCCATTTTTGCGATGAACTCCGGCACCATCCCGTCAAAACTCG‑CCATGTACTTGTCATTCCGCTCAATCACGACATAATTCAGGCCTTCACGCTTCATTCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTTCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATg                                                                                                                                                     <  2:557186/251‑1 (MQ=255)                                                                                           aaaTACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAtt                                                                                                                                                                                   <  1:12771/217‑1 (MQ=255)                                                                                           aaaTACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAtt                                                                                                                                                                                   >  2:12771/1‑217 (MQ=255)                                                                                              tACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCAGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATAGCCTCGAAACCACCGAGCCGGAATTTCATGAAAAccc                                                                                                                                              >  2:361741/1‑251 (MQ=255)                                                                                               gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAATCCGCCAAGGCGGAATTTCATGaagtcgcg                                                                                                                                             <  1:488434/236‑7 (MQ=255)                                                                                               gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGaagtcgcg                                                                                                                                             >  1:41739/16‑245 (MQ=255)                                                                                               gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGAAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGacgtcgcg                                                                                                                                             >  2:299569/16‑244 (MQ=255)                                                                                                     ccAATGTCAGCCAGTGCCCCGTCCATTTTTTCGATGAACTCCGGCACCACCTCGTCAAAACTCG‑CCATTGACTTTTCATACCGCTCAATCCCGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATATTTGGCAAAGTCCCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCCCCTGGGCCATGTAAGCCGATTTTATTGCCGCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGt                                                                                                                                       <  2:504267/251‑1 (MQ=255)                                                                                                      cgatctctgccagctccTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCgggggggg                                                                                                                                      >  1:133381/16‑246 (MQ=255)                                                                                                       aaTTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGCCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                     >  2:207742/1‑251 (MQ=255)                                                                                                            ctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaag                                                                                                                                  <  2:90955/247‑6 (MQ=255)                                                                                                             aGCCAGTGCCTCGTCCATTTTTTCGATGCACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGAAAAGTACCAGGCATCTTTTCGAGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACAGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTAt                                                                                                                               >  1:581002/1‑251 (MQ=255)                                                                                                              gCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggc                                                                                                                               >  1:300468/1‑242 (MQ=255)                                                                                                                cagcgCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatt                                                                                                                            <  1:14799/247‑12 (MQ=255)                                                                                                                cagcgCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatt                                                                                                                            >  2:35174/5‑240 (MQ=255)                                                                                                                 aGTGCCTCGTCCATTTTTTCGATGAACTCCGGCCCCATCCCGTCAAAACTCG‑CCCTGCTCTTTTCATTCCGCTCAATCCCGACATAATGCAGGCCTTCACGCTGCATGCGCGGGTCATAGTTGGCAAATTACCAGGCATCTTTTCGCGTCACCCACATGCTGTCCTGCACCTGGGCCATGTAAGCCTATTTTATTGCCTCGAAACCCCCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGcc                                                                                                                           <  2:581002/251‑1 (MQ=255)                                                                                                                    gCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCgg                                                                                                                         >  1:378170/1‑250 (MQ=255)                                                                                                                     ccTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagc                                                                                                                       >  1:547816/1‑235 (MQ=255)                                                                                                                       tCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagctc                                                                                                                     >  2:567252/1‑233 (MQ=255)                                                                                                                       ccGTCCATTTTTTCGATGAACCCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGc                                                                                                                     <  2:313570/250‑1 (MQ=255)                                                                                                                        cGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                     <  1:241430/234‑1 (MQ=255)                                                                                                                        cGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                     >  2:241430/1‑234 (MQ=255)                                                                                                                        cGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGTGAGGtgaacgggcatttaagccc                                                                                                                     >  2:192719/1‑232 (MQ=255)                                                                                                                         gTCCATTTTTTCGATGAACGCCGGCACCATCTCGTCAATACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTCCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCCCATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGt                                                                                                                   <  2:305749/251‑1 (MQ=255)                                                                                                                         gTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagctca                                                                                                                    >  1:27622/1‑231 (MQ=255)                                                                                                                             aTTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGcc                                                                                                               <  1:355379/251‑1 (MQ=255)                                                                                                                                      aTGAACTCCGGCAACATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGCGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCgg                                                                                                      >  2:71256/1‑251 (MQ=255)                                                                                                                                                         cGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATc                                                                                       <  1:356404/247‑1 (MQ=255)                                                                                                                                                              aaaCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGCCATCATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGCAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATGCGGATGCAGAGTCTTATCCGTGGAAAt                                                                              >  2:399859/1‑251 (MQ=255)                                                                                                                                                              aaaCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAAt                                                                              <  1:87431/251‑1 (MQ=255)                                                                                                                                                                aCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCg                                                                            <  1:32492/251‑1 (MQ=255)                                                                                                                                                                        aTGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACgcgc                                                                     >  2:324063/1‑251 (MQ=255)                                                                                                                                                                               tttCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtg                                                              >  1:21083/1‑251 (MQ=255)                                                                                                                                                                                  cATTCCGCGCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtggtt                                                           <  2:347144/251‑1 (MQ=255)                                                                                                                                                                                       cgcacAATCACGACATATTGCAGGCCCTCCCGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCTGTCATCTTTACACGGCACCCACATGCTGTACGGAACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCGGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTa                                                      <  2:137232/247‑1 (MQ=255)                                                                                                                                                                                           cAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCa                                                   <  1:547201/250‑1 (MQ=255)                                                                                                                                                                                                  gACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACAGGCTGTCCTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAc                                           <  2:501046/251‑1 (MQ=255)                                                                                                                                                                                                   aCATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAcc                                          <  1:568642/251‑1 (MQ=255)                                                                                                                                                                                                    cATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAcc                                          >  1:540178/1‑250 (MQ=255)                                                                                                                                                                                                             ggCCTGCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCGTCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGTAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCAATACTGGTTGGTTACCAACGTGTACCAGAACATGCg                                <  1:480548/251‑1 (MQ=255)                                                                                                                                                                                                                     cgctgcctgctcgGGTCTTATTTGGCAAAGTACCGGGCATCTTTGCGCGTCACCCACAGGCTGTTCTGCACCTTGGCCATGTAAGCCGATTTTATTGCCTCGAAACTGCCGAGTCGGAATTTCATGAAATCCCGGGAGTTACGAGTATTGCCTGATGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGTAAATCGAACGCGCATTGCTTGTTGGTTAACAACTTTTACCAAAACATGTGGGCCAATg                        <  2:555406/240‑1 (MQ=255)                                                                                                                                                                                                                       cgtCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCTCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcg                      <  2:211843/249‑1 (MQ=255)                                                                                                                                                                                                                        ttCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGCGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCACATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcgc                     >  2:205789/1‑251 (MQ=255)                                                                                                                                                                                                                         tCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcgc                     <  1:54614/250‑1 (MQ=255)                                                                                                                                                                                                                          cATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctg                   >  1:566084/1‑251 (MQ=255)                                                                                                                                                                                                                          cATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctg                   <  1:531313/251‑1 (MQ=255)                                                                                                                                                                                                                            tGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctggc                 >  2:513811/1‑251 (MQ=255)                                                                                                                                                                                                                                           tGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTGCGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACg  >  2:415259/1‑251 (MQ=255)                                                                                                                                                                                                                                           tGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCATTGCGCTGGCTGACGCGGAATTACg  >  1:362973/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           CAGCGGAGACTGAAGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACG  >  NC_000913/566026‑566509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑