Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	566,283	0	G	C	21.5%	152.5 / 44.7	67	intergenic (‑305/‑401)	intD/renD	putative integrase/protein RenD
Reads supporting (aligned to +/- strand):  ref base G (22/29);  new base C (10/4);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 7.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-02
Rejected as consensus: Frequency below/above cutoff threshold.

CTGAAGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACG  >  NC_000913/566035‑566509                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   cagAAGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGAACCCGGATATTATCGTGAGGATGCTTCATCGCCATTGCTCCCAAAATACGAAACCAATTTCAGCCATGGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTAGACAAAACACG‑AAATGTACTTTTCATTCCGCTCAATCACGACTTGATGCAGGCCTTCGCTCTCCCTGCGCGGGGCATAGTTAGCCAAGTACAAGTAATCTTTTcgcg                                                                                                                                                                                                                                  >  2:498403/3‑251 (MQ=255)       cgccgcAATGCAGGCTTGCCGTGTTGTGCGGTACCCGGATATTATCGTGAGGCTGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCCTCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGCCATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCATAGTACCAGGCATCTTTTcgcgaccccc                                                                                                                                                                                                                            >  2:387246/1‑245 (MQ=255)                gCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGt                                                                                                                                                                                                                   <  2:194199/251‑1 (MQ=255)                    gCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTg                                                                                                                                                                                                               <  1:396227/251‑1 (MQ=255)                           gtgtTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTGTTCGCGTCACCCACATGCTGTACTGCACCTgg                                                                                                                                                                                                        <  1:492051/251‑1 (MQ=255)                            tgttgtGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACCTGCTGTACTGCACCTggg                                                                                                                                                                                                       >  2:547201/1‑251 (MQ=255)                                 tgAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATg                                                                                                                                                                                                  >  2:151949/1‑251 (MQ=255)                                   aGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTa                                                                                                                                                                                                <  2:169600/251‑1 (MQ=255)                                   aGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAACCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCCTAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGt                                                                                                                                                                                                 >  2:312234/1‑250 (MQ=255)                                   aGGTACACGGATATTATCGTGAGGATGCGTCATCGCCGTTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTACGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCCCCTGGGCCATGt                                                                                                                                                                                                 >  2:98621/1‑250 (MQ=255)                                    ggTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTaa                                                                                                                                                                                               <  1:358946/251‑1 (MQ=255)                                        ccaggATATTATCGTGAGGCTGCGTCATCGCCATTGCTCCCCACATACAACACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCCCCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCCCCTGCTGTCCTGCCCCTGGGCCCTGTAGGCCg                                                                                                                                                                                           >  2:382817/4‑251 (MQ=255)                                            gATAGTATCGGGAGGCAGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCGGCCAGTTCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATCTACTTTTCATTCAGCTCAATCACGACATAATGCAGGGCTTCACGCTTCATGACCGGGACATAGTTGGCAAAGTACCAGGCCTCTTTTCGCGTCACCCACATGCTGTACTGACCCTCGGCCATGTAAGCCgctat                                                                                                                                                                                       >  2:35829/1‑247 (MQ=255)                                                  atCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTGTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTg                                                                                                                                                                                  <  2:153907/250‑1 (MQ=255)                                                     gTGATGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGGGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTc                                                                                                                                                                              <  2:465636/251‑1 (MQ=255)                                                     gTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTc                                                                                                                                                                              <  1:484961/251‑1 (MQ=255)                                                              cGTCAACGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAaccacc                                                                                                                                                                     <  1:153782/251‑1 (MQ=255)                                                                          tGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAAtt                                                                                                                                                          <  1:519864/250‑1 (MQ=255)                                                                            ttccccgaacgtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTc                                                                                                                                                        >  1:537628/26‑250 (MQ=255)                                                                              ccccgaacgtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCAt                                                                                                                                                      <  1:274275/227‑1 (MQ=255)                                                                              ccccAAATACAAAACCAATTCCAGCCAGTGCCTCGTCCATTTTTGCGATGAACTCCGGCACCATCCCGTCAAAACTCG‑CCATGTACTTGTCATTCCGCTCAATCACGACATAATTCAGGCCTTCACGCTTCATTCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTTCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATg                                                                                                                                                     <  2:557186/251‑1 (MQ=255)                                                                                  aaaTACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAtt                                                                                                                                                                                   <  1:12771/217‑1 (MQ=255)                                                                                  aaaTACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAtt                                                                                                                                                                                   >  2:12771/1‑217 (MQ=255)                                                                                     tACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCAGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATAGCCTCGAAACCACCGAGCCGGAATTTCATGAAAAccc                                                                                                                                              >  2:361741/1‑251 (MQ=255)                                                                                      gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAATCCGCCAAGGCGGAATTTCATGaagtcgcg                                                                                                                                             <  1:488434/236‑7 (MQ=255)                                                                                      gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGaagtcgcg                                                                                                                                             >  1:41739/16‑245 (MQ=255)                                                                                      gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGAAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGacgtcgcg                                                                                                                                             >  2:299569/16‑244 (MQ=255)                                                                                            ccAATGTCAGCCAGTGCCCCGTCCATTTTTTCGATGAACTCCGGCACCACCTCGTCAAAACTCG‑CCATTGACTTTTCATACCGCTCAATCCCGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATATTTGGCAAAGTCCCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCCCCTGGGCCATGTAAGCCGATTTTATTGCCGCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGt                                                                                                                                       <  2:504267/251‑1 (MQ=255)                                                                                             cgatctctgccagctccTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCgggggggg                                                                                                                                      >  1:133381/16‑246 (MQ=255)                                                                                              aaTTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGCCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                     >  2:207742/1‑251 (MQ=255)                                                                                                   ctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaag                                                                                                                                  <  2:90955/247‑6 (MQ=255)                                                                                                    aGCCAGTGCCTCGTCCATTTTTTCGATGCACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGAAAAGTACCAGGCATCTTTTCGAGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACAGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTAt                                                                                                                               >  1:581002/1‑251 (MQ=255)                                                                                                     gCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggc                                                                                                                               >  1:300468/1‑242 (MQ=255)                                                                                                       cagcgCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatt                                                                                                                            <  1:14799/247‑12 (MQ=255)                                                                                                       cagcgCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatt                                                                                                                            >  2:35174/5‑240 (MQ=255)                                                                                                        aGTGCCTCGTCCATTTTTTCGATGAACTCCGGCCCCATCCCGTCAAAACTCG‑CCCTGCTCTTTTCATTCCGCTCAATCCCGACATAATGCAGGCCTTCACGCTGCATGCGCGGGTCATAGTTGGCAAATTACCAGGCATCTTTTCGCGTCACCCACATGCTGTCCTGCACCTGGGCCATGTAAGCCTATTTTATTGCCTCGAAACCCCCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGcc                                                                                                                           <  2:581002/251‑1 (MQ=255)                                                                                                           gCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCgg                                                                                                                         >  1:378170/1‑250 (MQ=255)                                                                                                            ccTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagc                                                                                                                       >  1:547816/1‑235 (MQ=255)                                                                                                              tCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagctc                                                                                                                     >  2:567252/1‑233 (MQ=255)                                                                                                              ccGTCCATTTTTTCGATGAACCCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGc                                                                                                                     <  2:313570/250‑1 (MQ=255)                                                                                                               cGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                     <  1:241430/234‑1 (MQ=255)                                                                                                               cGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                     >  2:241430/1‑234 (MQ=255)                                                                                                               cGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGTGAGGtgaacgggcatttaagccc                                                                                                                     >  2:192719/1‑232 (MQ=255)                                                                                                                gTCCATTTTTTCGATGAACGCCGGCACCATCTCGTCAATACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTCCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCCCATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGt                                                                                                                   <  2:305749/251‑1 (MQ=255)                                                                                                                gTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagctca                                                                                                                    >  1:27622/1‑231 (MQ=255)                                                                                                                    aTTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGcc                                                                                                               <  1:355379/251‑1 (MQ=255)                                                                                                                             aTGAACTCCGGCAACATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGCGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCgg                                                                                                      >  2:71256/1‑251 (MQ=255)                                                                                                                                                cGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATc                                                                                       <  1:356404/247‑1 (MQ=255)                                                                                                                                                     aaaCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGCCATCATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGCAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATGCGGATGCAGAGTCTTATCCGTGGAAAt                                                                              >  2:399859/1‑251 (MQ=255)                                                                                                                                                     aaaCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAAt                                                                              <  1:87431/251‑1 (MQ=255)                                                                                                                                                       aCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCg                                                                            <  1:32492/251‑1 (MQ=255)                                                                                                                                                               aTGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACgcgc                                                                     >  2:324063/1‑251 (MQ=255)                                                                                                                                                                      tttCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtg                                                              >  1:21083/1‑251 (MQ=255)                                                                                                                                                                         cATTCCGCGCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtggtt                                                           <  2:347144/251‑1 (MQ=255)                                                                                                                                                                              cgcacAATCACGACATATTGCAGGCCCTCCCGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCTGTCATCTTTACACGGCACCCACATGCTGTACGGAACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCGGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTa                                                      <  2:137232/247‑1 (MQ=255)                                                                                                                                                                                  cAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCa                                                   <  1:547201/250‑1 (MQ=255)                                                                                                                                                                                         gACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACAGGCTGTCCTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAc                                           <  2:501046/251‑1 (MQ=255)                                                                                                                                                                                          aCATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAcc                                          <  1:568642/251‑1 (MQ=255)                                                                                                                                                                                           cATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAcc                                          >  1:540178/1‑250 (MQ=255)                                                                                                                                                                                                    ggCCTGCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCGTCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGTAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCAATACTGGTTGGTTACCAACGTGTACCAGAACATGCg                                <  1:480548/251‑1 (MQ=255)                                                                                                                                                                                                            cgctgcctgctcgGGTCTTATTTGGCAAAGTACCGGGCATCTTTGCGCGTCACCCACAGGCTGTTCTGCACCTTGGCCATGTAAGCCGATTTTATTGCCTCGAAACTGCCGAGTCGGAATTTCATGAAATCCCGGGAGTTACGAGTATTGCCTGATGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGTAAATCGAACGCGCATTGCTTGTTGGTTAACAACTTTTACCAAAACATGTGGGCCAATg                        <  2:555406/240‑1 (MQ=255)                                                                                                                                                                                                              cgtCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCTCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcg                      <  2:211843/249‑1 (MQ=255)                                                                                                                                                                                                               ttCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGCGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCACATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcgc                     >  2:205789/1‑251 (MQ=255)                                                                                                                                                                                                                tCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcgc                     <  1:54614/250‑1 (MQ=255)                                                                                                                                                                                                                 cATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctg                   >  1:566084/1‑251 (MQ=255)                                                                                                                                                                                                                 cATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctg                   <  1:531313/251‑1 (MQ=255)                                                                                                                                                                                                                   tGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctggc                 >  2:513811/1‑251 (MQ=255)                                                                                                                                                                                                                                  tGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTGCGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACg  >  2:415259/1‑251 (MQ=255)                                                                                                                                                                                                                                  tGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCATTGCGCTGGCTGACGCGGAATTACg  >  1:362973/1‑251 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   CTGAAGCGCCGCAATGCAGGCTTGCCGTGTTGTGAGGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACG  >  NC_000913/566035‑566509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑