Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	566,320	0	A	C	21.5%	131.0 / 28.1	66	intergenic (‑342/‑364)	intD/renD	putative integrase/protein RenD
Reads supporting (aligned to +/- strand):  ref base A (20/31);  new base C (10/4);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 3.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGAT  >  NC_000913/566070‑566569                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                          ggTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTaa                                                                                                                                                                                                                                                           <  1:358946/251‑1 (MQ=255)     ccaggATATTATCGTGAGGCTGCGTCATCGCCATTGCTCCCCACATACAACACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCCCCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCCCCTGCTGTCCTGCCCCTGGGCCCTGTAGGCCg                                                                                                                                                                                                                                                       >  2:382817/4‑251 (MQ=255)         gATAGTATCGGGAGGCAGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCGGCCAGTTCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATCTACTTTTCATTCAGCTCAATCACGACATAATGCAGGGCTTCACGCTTCATGACCGGGACATAGTTGGCAAAGTACCAGGCCTCTTTTCGCGTCACCCACATGCTGTACTGACCCTCGGCCATGTAAGCCgctat                                                                                                                                                                                                                                                   >  2:35829/1‑247 (MQ=255)               atCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTGTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTg                                                                                                                                                                                                                                              <  2:153907/250‑1 (MQ=255)                  gTGATGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGGGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTc                                                                                                                                                                                                                                          <  2:465636/251‑1 (MQ=255)                  gTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTc                                                                                                                                                                                                                                          <  1:484961/251‑1 (MQ=255)                           cGTCAACGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAaccacc                                                                                                                                                                                                                                 <  1:153782/251‑1 (MQ=255)                                       tGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAAtt                                                                                                                                                                                                                      <  1:519864/250‑1 (MQ=255)                                         ttccccgaacgtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTc                                                                                                                                                                                                                    >  1:537628/26‑250 (MQ=255)                                           ccccgaacgtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCAt                                                                                                                                                                                                                  <  1:274275/227‑1 (MQ=255)                                           ccccAAATACAAAACCAATTCCAGCCAGTGCCTCGTCCATTTTTGCGATGAACTCCGGCACCATCCCGTCAAAACTCG‑CCATGTACTTGTCATTCCGCTCAATCACGACATAATTCAGGCCTTCACGCTTCATTCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTTCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATg                                                                                                                                                                                                                 <  2:557186/251‑1 (MQ=255)                                               aaaTACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAtt                                                                                                                                                                                                                                               <  1:12771/217‑1 (MQ=255)                                               aaaTACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAtt                                                                                                                                                                                                                                               >  2:12771/1‑217 (MQ=255)                                                  tACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCAGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATAGCCTCGAAACCACCGAGCCGGAATTTCATGAAAAccc                                                                                                                                                                                                          >  2:361741/1‑251 (MQ=255)                                                   gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAATCCGCCAAGGCGGAATTTCATGaagtcgcg                                                                                                                                                                                                         <  1:488434/236‑7 (MQ=255)                                                   gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGaagtcgcg                                                                                                                                                                                                         >  1:41739/16‑245 (MQ=255)                                                   gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGAAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGacgtcgcg                                                                                                                                                                                                         >  2:299569/16‑244 (MQ=255)                                                         ccAATGTCAGCCAGTGCCCCGTCCATTTTTTCGATGAACTCCGGCACCACCTCGTCAAAACTCG‑CCATTGACTTTTCATACCGCTCAATCCCGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATATTTGGCAAAGTCCCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCCCCTGGGCCATGTAAGCCGATTTTATTGCCGCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGt                                                                                                                                                                                                   <  2:504267/251‑1 (MQ=255)                                                          cgatctctgccagctccTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCgggggggg                                                                                                                                                                                                  >  1:133381/16‑246 (MQ=255)                                                           aaTTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGCCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                                                                                 >  2:207742/1‑251 (MQ=255)                                                                ctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaag                                                                                                                                                                                              <  2:90955/247‑6 (MQ=255)                                                                 aGCCAGTGCCTCGTCCATTTTTTCGATGCACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGAAAAGTACCAGGCATCTTTTCGAGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACAGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTAt                                                                                                                                                                                           >  1:581002/1‑251 (MQ=255)                                                                  gCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggc                                                                                                                                                                                           >  1:300468/1‑242 (MQ=255)                                                                    cagcgCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatt                                                                                                                                                                                        <  1:14799/247‑12 (MQ=255)                                                                    cagcgCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatt                                                                                                                                                                                        >  2:35174/5‑240 (MQ=255)                                                                     aGTGCCTCGTCCATTTTTTCGATGAACTCCGGCCCCATCCCGTCAAAACTCG‑CCCTGCTCTTTTCATTCCGCTCAATCCCGACATAATGCAGGCCTTCACGCTGCATGCGCGGGTCATAGTTGGCAAATTACCAGGCATCTTTTCGCGTCACCCACATGCTGTCCTGCACCTGGGCCATGTAAGCCTATTTTATTGCCTCGAAACCCCCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGcc                                                                                                                                                                                       <  2:581002/251‑1 (MQ=255)                                                                        gCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCgg                                                                                                                                                                                     >  1:378170/1‑250 (MQ=255)                                                                         ccTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagc                                                                                                                                                                                   >  1:547816/1‑235 (MQ=255)                                                                           tCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagctc                                                                                                                                                                                 >  2:567252/1‑233 (MQ=255)                                                                           ccGTCCATTTTTTCGATGAACCCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGc                                                                                                                                                                                 <  2:313570/250‑1 (MQ=255)                                                                            cGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                                                                                 <  1:241430/234‑1 (MQ=255)                                                                            cGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                                                                                 >  2:241430/1‑234 (MQ=255)                                                                            cGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGTGAGGtgaacgggcatttaagccc                                                                                                                                                                                 >  2:192719/1‑232 (MQ=255)                                                                             gTCCATTTTTTCGATGAACGCCGGCACCATCTCGTCAATACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTCCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCCCATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGt                                                                                                                                                                               <  2:305749/251‑1 (MQ=255)                                                                             gTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagctca                                                                                                                                                                                >  1:27622/1‑231 (MQ=255)                                                                                 aTTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGcc                                                                                                                                                                           <  1:355379/251‑1 (MQ=255)                                                                                          aTGAACTCCGGCAACATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGCGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCgg                                                                                                                                                                  >  2:71256/1‑251 (MQ=255)                                                                                                             cGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATc                                                                                                                                                   <  1:356404/247‑1 (MQ=255)                                                                                                                  aaaCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGCCATCATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGCAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATGCGGATGCAGAGTCTTATCCGTGGAAAt                                                                                                                                          >  2:399859/1‑251 (MQ=255)                                                                                                                  aaaCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAAt                                                                                                                                          <  1:87431/251‑1 (MQ=255)                                                                                                                    aCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCg                                                                                                                                        <  1:32492/251‑1 (MQ=255)                                                                                                                            aTGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACgcgc                                                                                                                                 >  2:324063/1‑251 (MQ=255)                                                                                                                                   tttCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtg                                                                                                                          >  1:21083/1‑251 (MQ=255)                                                                                                                                      cATTCCGCGCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtggtt                                                                                                                       <  2:347144/251‑1 (MQ=255)                                                                                                                                           cgcacAATCACGACATATTGCAGGCCCTCCCGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCTGTCATCTTTACACGGCACCCACATGCTGTACGGAACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCGGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTa                                                                                                                  <  2:137232/247‑1 (MQ=255)                                                                                                                                               cAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCa                                                                                                               <  1:547201/250‑1 (MQ=255)                                                                                                                                                      gACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACAGGCTGTCCTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAc                                                                                                       <  2:501046/251‑1 (MQ=255)                                                                                                                                                       aCATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAcc                                                                                                      <  1:568642/251‑1 (MQ=255)                                                                                                                                                        cATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAcc                                                                                                      >  1:540178/1‑250 (MQ=255)                                                                                                                                                                 ggCCTGCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCGTCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGTAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCAATACTGGTTGGTTACCAACGTGTACCAGAACATGCg                                                                                            <  1:480548/251‑1 (MQ=255)                                                                                                                                                                         cgctgcctgctcgGGTCTTATTTGGCAAAGTACCGGGCATCTTTGCGCGTCACCCACAGGCTGTTCTGCACCTTGGCCATGTAAGCCGATTTTATTGCCTCGAAACTGCCGAGTCGGAATTTCATGAAATCCCGGGAGTTACGAGTATTGCCTGATGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGTAAATCGAACGCGCATTGCTTGTTGGTTAACAACTTTTACCAAAACATGTGGGCCAATg                                                                                    <  2:555406/240‑1 (MQ=255)                                                                                                                                                                           cgtCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCTCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcg                                                                                  <  2:211843/249‑1 (MQ=255)                                                                                                                                                                            ttCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGCGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCACATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcgc                                                                                 >  2:205789/1‑251 (MQ=255)                                                                                                                                                                             tCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcgc                                                                                 <  1:54614/250‑1 (MQ=255)                                                                                                                                                                              cATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctg                                                                               <  1:531313/251‑1 (MQ=255)                                                                                                                                                                              cATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctg                                                                               >  1:566084/1‑251 (MQ=255)                                                                                                                                                                                tGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctggc                                                                             >  2:513811/1‑251 (MQ=255)                                                                                                                                                                                               tGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTGCGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACg                                                              >  2:415259/1‑251 (MQ=255)                                                                                                                                                                                               tGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCATTGCGCTGGCTGACGCGGAATTACg                                                              >  1:362973/1‑251 (MQ=255)                                                                                                                                                                                                                         gTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCt                                    >  1:154298/1‑251 (MQ=255)                                                                                                                                                                                                                         gTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCt                                    <  1:447291/251‑1 (MQ=255)                                                                                                                                                                                                                                acaTGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTGCGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGAc                             <  1:415259/251‑1 (MQ=255)                                                                                                                                                                                                                                   tGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGAATGACAgc                          >  2:361945/1‑251 (MQ=255)                                                                                                                                                                                                                                            gCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAAtt                   <  1:30997/249‑1 (MQ=255)                                                                                                                                                                                                                                              cccTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAAcc               <  1:151949/250‑1 (MQ=255)                                                                                                                                                                                                                                               ccTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCg              <  1:387246/251‑1 (MQ=255)                                                                                                                                                                                                                                                         gttaGCCGATTTTTGTGCCCCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGTAGCAGAGTCGTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACg    <  2:394740/248‑1 (MQ=255)                                                                                                                                                                                                                                                         gTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCGGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGGGAGACg    >  2:372984/1‑251 (MQ=255)                                                                                                                                                                                                                                                           aaGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGAt  <  1:4011/251‑1 (MQ=255)                                                                                                                                                                                                                                                           aaGCCGATTTTATTGCCCCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGAt  <  2:154298/251‑1 (MQ=255)                                                                                                                                                                                                                                                            aGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGAt  >  2:473140/1‑250 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                          GGTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGAT  >  NC_000913/566070‑566569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑