Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	566,332	0	T	G	20.9%	123.9 / 44.2	68	intergenic (‑354/‑352)	intD/renD	putative integrase/protein RenD
Reads supporting (aligned to +/- strand):  ref base T (18/35);  new base G (10/4);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 1.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGT  >  NC_000913/566084‑566581                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                          atCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTGTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTg                                                                                                                                                                                                                                                          <  2:153907/250‑1 (MQ=255)    gTGATGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGGGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTc                                                                                                                                                                                                                                                      <  2:465636/251‑1 (MQ=255)    gTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTc                                                                                                                                                                                                                                                      <  1:484961/251‑1 (MQ=255)             cGTCAACGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAaccacc                                                                                                                                                                                                                                             <  1:153782/251‑1 (MQ=255)                         tGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAAtt                                                                                                                                                                                                                                  <  1:519864/250‑1 (MQ=255)                           ttccccgaacgtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTc                                                                                                                                                                                                                                >  1:537628/26‑250 (MQ=255)                             ccccgaacgtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCAt                                                                                                                                                                                                                              <  1:274275/227‑1 (MQ=255)                             ccccAAATACAAAACCAATTCCAGCCAGTGCCTCGTCCATTTTTGCGATGAACTCCGGCACCATCCCGTCAAAACTCG‑CCATGTACTTGTCATTCCGCTCAATCACGACATAATTCAGGCCTTCACGCTTCATTCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTTCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATg                                                                                                                                                                                                                             <  2:557186/251‑1 (MQ=255)                                 aaaTACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAtt                                                                                                                                                                                                                                                           <  1:12771/217‑1 (MQ=255)                                 aaaTACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAtt                                                                                                                                                                                                                                                           >  2:12771/1‑217 (MQ=255)                                    tACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCAGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATAGCCTCGAAACCACCGAGCCGGAATTTCATGAAAAccc                                                                                                                                                                                                                      >  2:361741/1‑251 (MQ=255)                                     gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAATCCGCCAAGGCGGAATTTCATGaagtcgcg                                                                                                                                                                                                                     <  1:488434/236‑7 (MQ=255)                                     gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGaagtcgcg                                                                                                                                                                                                                     >  1:41739/16‑245 (MQ=255)                                     gtgaagccgatctctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGAAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGacgtcgcg                                                                                                                                                                                                                     >  2:299569/16‑244 (MQ=255)                                           ccAATGTCAGCCAGTGCCCCGTCCATTTTTTCGATGAACTCCGGCACCACCTCGTCAAAACTCG‑CCATTGACTTTTCATACCGCTCAATCCCGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATATTTGGCAAAGTCCCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCCCCTGGGCCATGTAAGCCGATTTTATTGCCGCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGt                                                                                                                                                                                                               <  2:504267/251‑1 (MQ=255)                                            cgatctctgccagctccTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCgggggggg                                                                                                                                                                                                              >  1:133381/16‑246 (MQ=255)                                             aaTTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGCCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                                                                                             >  2:207742/1‑251 (MQ=255)                                                  ctgCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaag                                                                                                                                                                                                          <  2:90955/247‑6 (MQ=255)                                                   aGCCAGTGCCTCGTCCATTTTTTCGATGCACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGAAAAGTACCAGGCATCTTTTCGAGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACAGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTAt                                                                                                                                                                                                       >  1:581002/1‑251 (MQ=255)                                                    gCCAGCGCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggc                                                                                                                                                                                                       >  1:300468/1‑242 (MQ=255)                                                      cagcgCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatt                                                                                                                                                                                                    <  1:14799/247‑12 (MQ=255)                                                      cagcgCCTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatt                                                                                                                                                                                                    >  2:35174/5‑240 (MQ=255)                                                       aGTGCCTCGTCCATTTTTTCGATGAACTCCGGCCCCATCCCGTCAAAACTCG‑CCCTGCTCTTTTCATTCCGCTCAATCCCGACATAATGCAGGCCTTCACGCTGCATGCGCGGGTCATAGTTGGCAAATTACCAGGCATCTTTTCGCGTCACCCACATGCTGTCCTGCACCTGGGCCATGTAAGCCTATTTTATTGCCTCGAAACCCCCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGcc                                                                                                                                                                                                   <  2:581002/251‑1 (MQ=255)                                                          gCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCgg                                                                                                                                                                                                 >  1:378170/1‑250 (MQ=255)                                                           ccTCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagc                                                                                                                                                                                               >  1:547816/1‑235 (MQ=255)                                                             tCGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagctc                                                                                                                                                                                             >  2:567252/1‑233 (MQ=255)                                                             ccGTCCATTTTTTCGATGAACCCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGc                                                                                                                                                                                             <  2:313570/250‑1 (MQ=255)                                                              cGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                                                                                             <  1:241430/234‑1 (MQ=255)                                                              cGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                                                                                                                                                                                             >  2:241430/1‑234 (MQ=255)                                                              cGTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGTGAGGtgaacgggcatttaagccc                                                                                                                                                                                             >  2:192719/1‑232 (MQ=255)                                                               gTCCATTTTTTCGATGAACGCCGGCACCATCTCGTCAATACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTCCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCCCATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGt                                                                                                                                                                                           <  2:305749/251‑1 (MQ=255)                                                               gTCCATCTTCTCGATGAACTCCGGCACCATTTCGTTGAAA‑TCGGACATGTACTGAGGATCCCGCTCAACGACGACGTGGTGAATGCCTTCGCGTTTCATGCGCGGGTCGTAGTTTGCAAAGAACCATGCGTCTTTTCCGGTCACCCACATGCTGTACTGCACCTGGGCCATGTACGCAGACTTGATGGCTTCGAAACCGCCAAGGCGGAATTTCATGAAGTCGCGGGAGGtgaaagggcatttaagctca                                                                                                                                                                                            >  1:27622/1‑231 (MQ=255)                                                                   aTTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGcc                                                                                                                                                                                       <  1:355379/251‑1 (MQ=255)                                                                            aTGAACTCCGGCAACATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGCGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCgg                                                                                                                                                                              >  2:71256/1‑251 (MQ=255)                                                                                               cGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATc                                                                                                                                                               <  1:356404/247‑1 (MQ=255)                                                                                                    aaaCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGCCATCATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGCAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATGCGGATGCAGAGTCTTATCCGTGGAAAt                                                                                                                                                      >  2:399859/1‑251 (MQ=255)                                                                                                    aaaCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAAt                                                                                                                                                      <  1:87431/251‑1 (MQ=255)                                                                                                      aCTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCg                                                                                                                                                    <  1:32492/251‑1 (MQ=255)                                                                                                              aTGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACgcgc                                                                                                                                             >  2:324063/1‑251 (MQ=255)                                                                                                                     tttCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtg                                                                                                                                      >  1:21083/1‑251 (MQ=255)                                                                                                                        cATTCCGCGCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACtggtt                                                                                                                                   <  2:347144/251‑1 (MQ=255)                                                                                                                             cgcacAATCACGACATATTGCAGGCCCTCCCGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCTGTCATCTTTACACGGCACCCACATGCTGTACGGAACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCGGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTa                                                                                                                              <  2:137232/247‑1 (MQ=255)                                                                                                                                 cAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCa                                                                                                                           <  1:547201/250‑1 (MQ=255)                                                                                                                                        gACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACAGGCTGTCCTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAc                                                                                                                   <  2:501046/251‑1 (MQ=255)                                                                                                                                         aCATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAcc                                                                                                                  <  1:568642/251‑1 (MQ=255)                                                                                                                                          cATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTAcc                                                                                                                  >  1:540178/1‑250 (MQ=255)                                                                                                                                                   ggCCTGCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCGTCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGTAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCAATACTGGTTGGTTACCAACGTGTACCAGAACATGCg                                                                                                        <  1:480548/251‑1 (MQ=255)                                                                                                                                                           cgctgcctgctcgGGTCTTATTTGGCAAAGTACCGGGCATCTTTGCGCGTCACCCACAGGCTGTTCTGCACCTTGGCCATGTAAGCCGATTTTATTGCCTCGAAACTGCCGAGTCGGAATTTCATGAAATCCCGGGAGTTACGAGTATTGCCTGATGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGTAAATCGAACGCGCATTGCTTGTTGGTTAACAACTTTTACCAAAACATGTGGGCCAATg                                                                                                <  2:555406/240‑1 (MQ=255)                                                                                                                                                             cgtCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCTCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcg                                                                                              <  2:211843/249‑1 (MQ=255)                                                                                                                                                              ttCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGCGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCACATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcgc                                                                                             >  2:205789/1‑251 (MQ=255)                                                                                                                                                               tCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATgcgc                                                                                             <  1:54614/250‑1 (MQ=255)                                                                                                                                                                cATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctg                                                                                           >  1:566084/1‑251 (MQ=255)                                                                                                                                                                cATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctg                                                                                           <  1:531313/251‑1 (MQ=255)                                                                                                                                                                  tGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCgctggc                                                                                         >  2:513811/1‑251 (MQ=255)                                                                                                                                                                                 tGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTGCGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACg                                                                          >  2:415259/1‑251 (MQ=255)                                                                                                                                                                                 tGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCATTGCGCTGGCTGACGCGGAATTACg                                                                          >  1:362973/1‑251 (MQ=255)                                                                                                                                                                                                           gTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCt                                                <  1:447291/251‑1 (MQ=255)                                                                                                                                                                                                           gTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCt                                                >  1:154298/1‑251 (MQ=255)                                                                                                                                                                                                                  acaTGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTGCGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGAc                                         <  1:415259/251‑1 (MQ=255)                                                                                                                                                                                                                     tGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGAATGACAgc                                      >  2:361945/1‑251 (MQ=255)                                                                                                                                                                                                                              gCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAAtt                               <  1:30997/249‑1 (MQ=255)                                                                                                                                                                                                                                cccTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAAcc                           <  1:151949/250‑1 (MQ=255)                                                                                                                                                                                                                                 ccTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCg                          <  1:387246/251‑1 (MQ=255)                                                                                                                                                                                                                                           gttaGCCGATTTTTGTGCCCCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGTAGCAGAGTCGTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACg                <  2:394740/248‑1 (MQ=255)                                                                                                                                                                                                                                           gTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCGGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGGGAGACg                >  2:372984/1‑251 (MQ=255)                                                                                                                                                                                                                                             aaGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGAt              <  1:4011/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaGCCGATTTTATTGCCCCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGAt              <  2:154298/251‑1 (MQ=255)                                                                                                                                                                                                                                              aGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGAt              >  2:473140/1‑250 (MQ=255)                                                                                                                                                                                                                                                  gATTTTATTGCCTCTAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGATTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTg         <  1:98621/251‑1 (MQ=255)                                                                                                                                                                                                                                                       tATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATTCCTGAAcc     <  1:146194/250‑1 (MQ=255)                                                                                                                                                                                                                                                          tGCCTCGAAACCACCGAGCCTGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGATCCAGt  <  2:378170/250‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                          ATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAATTTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCG‑CCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCCAATGCGCTGGCTGACGCGGAATTACGGCGCAAGGCTGCCGATGAACTGACCTGTATGACAGCGCGAATTAACCGTGGTGAGACGATACCTGAACCAGT  >  NC_000913/566084‑566581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑