Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,878,9470CT40.0% 25.4 / 12.8 30E276K (GAA→AAA) cas1multifunctional nuclease Cas1
Reads supporting (aligned to +/- strand):  ref base C (1/17);  new base T (12/0);  total (13/17)
Fisher's exact test for biased strand distribution p-value = 1.50e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTCCAGCTATTTGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATAAC  >  NC_000913/2878705‑2879177
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          
aGTCCAGCTATTTGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑GTGTTTTTTTcccgacg                                                                                                                                                                                                                                    >  2:6773/1‑246 (MQ=255)
            tGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACCTTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑ATGTGTTTCTCCCC‑CGGCAAGccactccc                                                                                                                                                                                                                       >  2:139551/1‑244 (MQ=255)
            tGTTCCCAGATCATTTCACGAATTTTTGCGAATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTcc                                                                                                                                                                                                                        <  1:483425/250‑1 (MQ=255)
                     aTCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑GTGTATTTTTCCAG‑Cgggcaacacgacctcctttagcg                                                                                                                                                                                                              >  1:236854/1‑230 (MQ=255)
                                           aTACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGGATTTTTCAAG‑CGGCCAACACGTCCTCTATAAG‑Cgggtacacttttgagaaagtttt                                                                                                                                                                                       >  1:135808/1‑230 (MQ=255)
                                             acaccACCTACATCTCCCCCTGCACGTACCACCAACAACCAGCTGGCTAATCTGCCTCGTAAGCGCGGAGGTACATGTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCGGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGTTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGTCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTGATGTTTta                                                                                                                                                                                     <  2:274228/246‑1 (MQ=255)
                                                  aCCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTTTCCAT‑CGGCAAGCACGTCCCCTCTAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑g                                                                                                                                                                              >  1:121342/1‑251 (MQ=255)
                                                      aCATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTTTCCAG‑CGGCCAGCACGTCCTCTATAAG‑CGGGATCAA‑TTTTGGTAATGTTTTACTACT‑GCgg                                                                                                                                                                           >  1:93727/1‑249 (MQ=255)
                                                        atatACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTTTCCCA‑CGGGAAGCACCGCCTCTATAAG‑CGGGATCCA‑TTTGGCTCATGTTTTACTACT‑GCGaaa                                                                                                                                                                         >  1:333523/1‑250 (MQ=255)
                                                                   cACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTTTCCCC‑CGGCAAGCACGGCCTCTATAAA‑CGGAAACCA‑TTTGGCTCATGTTTTACTACT‑GCGaaaaaaataccctc                                                                                                                                                              >  1:20270/1‑241 (MQ=255)
                                                                   cACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCa                                                                                                                                                             <  1:6506/251‑1 (MQ=255)
                                                                         ccTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGGTTGT‑TTTTTTCAGACGGCA‑GCACCGCCTCCATTAG‑CGGGATAAA‑TGTTGCGAATGTTTTTCTACT‑TCGCAAAATATTCCTGCACGCCa                                                                                                                                                        >  1:64867/1‑250 (MQ=255)
                                                                                        tGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTTTCCAC‑CGGCAAACACGCCCTCTATAAAAGGGAAT‑AA‑ATTAGCTGATGGTTTACTACT‑GCGAAAAATTTACCTGCCTGCCaaaacgagttaccggtc                                                                                                                                        >  1:294236/1‑237 (MQ=255)
                                                                                                cTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTTTTTTTTCCG‑CCGCCAACACCCCCTCTATAAA‑CGGAATAAA‑TTTTGGTTATTTTTTTCTACTTCCGAAAAA‑ATACCCCCACACCAAACAGACTTCCCCGTCCGGCTccc                                                                                                                                >  2:514119/1‑249 (MQ=255)
                                                                                                  gCCTCGTAAGCGCGGATGTACATTTTCAGTGACCACTACCAACATACTCATTTCAGCTACTCCTATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCTAAAAATATCCCTGCTCGCCAAACGGACTTCCCGGTCCGGCTCAcc                                                                                                                               <  2:94334/250‑1 (MQ=255)
                                                                                                     tCGTAAGCGCGGAGGTAAATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGATTG‑AGTTTTCCAA‑CGGCAAACACGTCCCCTATAAG‑AGGGATCAAATTTGG‑TGATGTTTTATTACA‑GTGGAAAAAATACCTTCACGCCAAAAGGGCTTTCCGGTACGGCCCAcccagg                                                                                                                           >  1:462271/1‑247 (MQ=255)
                                                                                                                          ttCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATAGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGTCGCGCTATCTCaaa                                                                                                       <  1:60643/250‑1 (MQ=255)
                                                                                                                                                  cATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAAt                                                                              <  1:6773/251‑1 (MQ=255)
                                                                                                                                                         gCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAatga                                                                       <  1:467112/251‑1 (MQ=255)
                                                                                                                                                                    ggCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGTGGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAata                                                            <  2:93727/251‑1 (MQ=255)
                                                                                                                                                                     gCCTGCAACTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAatat                                                           <  2:121342/251‑1 (MQ=255)
                                                                                                                                                                         gCATCTCCCAGGGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTa                                                       <  2:105077/251‑1 (MQ=255)
                                                                                                                                                                          cATCTCCCAGTGAAACAGTAATCGTAATGGCAACAGGCTGTGCATCTGCAGGTTGGGCCGGCAG‑TTGTATTACTCCAG‑CGGCAAGCACGGCCTCAATAAG‑CGGAATCAA‑TTGGGCTAATGTTTTTCTCCT‑GCGACAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACTCGCTATCTCAAAAGCTGTCGGTACAACAGTGTCAAATTTAATGATGTCAGCAATAGCGTaa                                                      <  2:577316/251‑1 (MQ=255)
                                                                                                                                                                                                  gAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTa                              <  1:522971/251‑1 (MQ=255)
                                                                                                                                                                                                      ggCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGca                          <  1:258634/251‑1 (MQ=255)
                                                                                                                                                                                                       gCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTTTCCCG‑CGGCAAGCACGGCCTCTATAAG‑CGGGATCAA‑ATTGGCTAATGTTTTACTACT‑GCGGAAAAAATCCCCGCACGCCAAACGGACTTCCCGGTCCCGCTCACCAGGGTTAACACGCGCTATCTCAAAAGCTTTCGGGACAACAAGGTCAAATTTAATGATTTCTGCAAAATAGGAAACAAAGGAAAGAGGCTTTCCTGTATGcac                         >  1:141974/1‑251 (MQ=255)
                                                                                                                                                                                                                 gtgCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAAt                <  1:211252/250‑1 (MQ=255)
                                                                                                                                                                                                                    cATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCt            <  2:20270/251‑1 (MQ=255)
                                                                                                                                                                                                                         tCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCGTTCCTGTATGCACAAACCCAATAGCTgg          <  1:501004/248‑1 (MQ=255)
                                                                                                                                                                                                                               ggtgCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATAAc  <  2:333523/247‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          
AGTCCAGCTATTTGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGG‑TTGTATTTCTCCAG‑CGGCAAGCACGTCCTCTATAAG‑CGGAATCAA‑TTTGGCTAATGTTTTACTACT‑GCGAAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATAAC  >  NC_000913/2878705‑2879177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑