breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 71 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091383,5791.A78.6% 101.0 / 22.0 42intergenic (‑50/+43)leuA/leuL2‑isopropylmalate synthase/leu operon leader peptide
*NC_0009132,909,6020GA59.6% 34.1 / 39.6 49A22V (GCA→GTA) pyrGCTP synthetase
*NC_000913139,9880A.49.5% 175.6 / 184.3 96coding (1238/2391 nt)gcdquinoprotein glucose dehydrogenase
*NC_000913811,7210TG45.0% 79.2 / 15.2 82V67G (GTG→GGG) bioCmalonyl‑acyl carrier protein methyltransferase
*NC_0009134,377,5500TC36.3% 86.6 / 98.3 94E58G (GAA→GGA) blcouter membrane lipoprotein Blc
*NC_000913803,8690GT36.1% 30.3 / 17.0 36V123L (GTG→TTG) ybhJputative hydratase YbhJ
*NC_0009133,901,8170C.35.6% 138.1 / 103.0 75coding (404/1617 nt)bglHcarbohydrate‑specific outer membrane porin, cryptic
*NC_0009134,622,2500AG35.1% 91.6 / 17.6 57E161G (GAG→GGG) yjjJtoxin YjjJ
*NC_0009131,435,7250CA34.3% 40.1 / 17.1 35intergenic (‑106/+35)uspF/ompNuniversal stress protein F/outer membrane porin N
*NC_0009131,435,7270GA34.3% 46.8 / 19.3 35intergenic (‑108/+33)uspF/ompNuniversal stress protein F/outer membrane porin N
*NC_000913948,5280AG34.2% 52.2 / 32.5 44I434V (ATC→GTC) ycaMputative transporter YcaM
*NC_000913119,3840TG33.9% 81.7 / 11.5 60V35G (GTG→GGG) ampEprotein AmpE
*NC_000913339,6210AG33.0% 111.0 / 82.5 94I433V (ATC→GTC) yahGDUF1116 domain‑containing protein YahG
*NC_0009134,027,2330TA32.8% 83.2 / 21.3 61intergenic (+5/+376)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
*NC_000913308,5940TG32.3% 91.9 / 14.4 68T247P (ACC→CCC) ecpCputative fimbrial usher protein EcpC
*NC_000913599,5760TG32.2% 77.1 / 14.1 59V288G (GTG→GGG) cusAcopper/silver export system RND permease
*NC_0009133,788,0250TG32.2% 166.4 / 17.1 115G396G (GGT→GGGenvCmurein hydrolase activator EnvC
*NC_0009133,458,2320TA31.0% 125.4 / 66.5 90I619N (ATC→AAC) gspDType II secretion system protein GspD
*NC_0009132,337,7230AC30.4% 79.8 / 13.4 58G566G (GGT→GGGgyrADNA gyrase subunit A
*NC_0009133,883,2440GA30.3% 127.9 / 94.4 111G162G (GGC→GGTdnaAchromosomal replication initiator protein DnaA

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3958551145 (2.040)3 (0.040) 3/488 3.9 2.2% coding (582/1476 nt) ilvC ketol‑acid reductoisomerase (NADP(+))
?NC_000913 4626635 = 126 (1.780)coding (724/1383 nt) radA DNA recombination protein
* ? NC_000913 3428156 =NA (NA)3 (0.040)
+T
3/488 3.9 3.3% noncoding (607/1542 nt) rrsD 16S ribosomal RNA
?NC_000913 3595835 = 88 (1.240)coding (569/927 nt) livH branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH
* ? NC_000913 = 256339336 (0.510)3 (0.040) 3/488 3.9 5.8% intergenic (+276/‑199) yffP/yffQ uncharacterized protein YffP/uncharacterized protein YffQ
?NC_000913 = 3201007 62 (0.880)intergenic (‑42/‑200) ygiF/ygiM inorganic triphosphatase/putative signal transduction protein (SH3 domain)