Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,788,0250TG32.2% 166.4 / 17.1 115G396G (GGT→GGG) envCmurein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base T (15/63);  new base G (35/2);  total (50/65)
Fisher's exact test for biased strand distribution p-value = 2.53e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 115 total aligned reads displayed.

GCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTGGCGATGCCTTCCGCTATCTCCGTCGCTGTATTACCCGATTCA  >  NC_000913/3787776‑3788274
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                               
gCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑Tc                                                                                                                                                                                                                                                                <  1:435389/251‑1 (MQ=255)
    cAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCgggc                                                                                                                                                                                                                                                            >  1:493212/1‑249 (MQ=255)
     aGGGTGAATTACGCTGGAAAGGTATGTTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑t                                                                                                                                                                                                                                                          <  1:470159/251‑1 (MQ=255)
          gAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCTGGG‑GCGGGC‑TTCAcc                                                                                                                                                                                                                                                     >  1:466293/1‑250 (MQ=255)
            aTTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGCACCGAAACTAAAGCGATTGCCGCAGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTATGGTGGTGGTGG‑TTGAGAATGGTAAAAGCGACATGA‑GTCTTTACGGATATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGGCAGGG‑TCGGGC‑TTCActct                                                                                                                                                                                                                                                   >  1:548759/1‑251 (MQ=255)
                 gCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGCAGCTAAAGCGATTGCCGACGGTCGGGCGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCCGCC‑TTCACTCTAtttt                                                                                                                                                                                                                                              >  1:248745/1‑250 (MQ=255)
                           tATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACCCTATTT‑CGGAAATcgcc                                                                                                                                                                                                                                   >  1:244828/1‑251 (MQ=255)
                                ttATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑Tc                                                                                                                                                                                                                                                                <  1:261794/219‑1 (MQ=255)
                                ttATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTTACTCTATTT‑TGGAATTTgccgcccg                                                                                                                                                                                                                              >  1:421331/1‑249 (MQ=255)
                                ttATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACTCTATTT‑CGGAATTcgccggccg                                                                                                                                                                                                                              >  1:85291/1‑247 (MQ=255)
                                     ggTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCCCTTCA‑TCTTTTTTGGAAATT‑GCCGCCAgggggc                                                                                                                                                                                                                         >  1:132120/1‑248 (MQ=255)
                                         cTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCCgggggcggcg                                                                                                                                                                                                                     >  1:556129/1‑244 (MQ=255)
                                                gggTACTGAAGTAAAAGCGATTGCCGACGGGCGGGTGAGTCTGGCTGACTGGATGCGAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAAGTCGCCGCCAGGGTCAGGCGGT‑CAATc                                                                                                                                                                                                             <  2:440066/250‑1 (MQ=255)
                                                aGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCTGGT‑AGGG‑GCGGTC‑TTCACTCTTTTT‑CCAGAATCGCCGCCAGGGTCAGGCGGG‑CAATc                                                                                                                                                                                                             >  2:4946/1‑251 (MQ=255)
                                                 ggTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCCGGG‑TCGGCC‑CTCACCCTTTTT‑TGAAATTCGCCGCCCCGGGCAGGCGGT‑CAATcc                                                                                                                                                                                                            >  1:133175/1‑251 (MQ=255)
                                                       gAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCCCTCTATTT‑CGAAATTTGCCGCCCGGGGCCGGCGGT‑CAATCCACCGcc                                                                                                                                                                                                      >  1:39449/1‑251 (MQ=255)
                                                       gAAGTAAAAGCAATTGCGGACGGGCGGTTGATTCTGGCGGACGGGCTGCAAGGCGACGGTCGGGTGGTGTGGG‑TGGAGGATGGTAAAGGCGACAGGA‑GACTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGGACGCGCGGGCCAGCCGAGTGCACGGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACGCTATTG‑CGAAATTCGCGGCCAGGGTCAGCCGAT‑CAATCCACAGcc                                                                                                                                                                                                      <  2:606567/251‑1 (MQ=255)
                                                        aaGTTAAAGCGATTGACGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCACGG‑TCTGCC‑CTCGCTCCATTT‑CGACATTCTCCGCCCGGTGCAGGCGGG‑CCATCcaccaccc                                                                                                                                                                                                     >  2:305193/1‑246 (MQ=255)
                                                             aaaGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATGT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGtt                                                                                                                                                                                                <  1:219442/251‑1 (MQ=255)
                                                             aaaGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GGGGCC‑TTCACTCTTTTT‑CGAAATTTGCCCCCACGGGCAGGCGGG‑CCATCCCCAGCCGTgggg                                                                                                                                                                                                >  1:316660/1‑249 (MQ=255)
                                                                  gATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGGC‑TTCACTCTATTT‑TGGAATTCGCCGCCAGGGGCAGGCGGT‑CAATCCACAGCCGTGGtttggg                                                                                                                                                                                            >  1:252976/1‑246 (MQ=255)
                                                                  gATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑CTCACTCTATTT‑CGCAATTCGCCCCCCGGGGCAGGGGGT‑CCATTCACAGCCGTGGTTgggg                                                                                                                                                                                            >  1:459202/1‑249 (MQ=255)
                                                                   aTTGCCGACGGTCGGGTGATTCTGGCTGACTGGCGGCAAGGCTACGGTCTGGTGGTGGTGG‑TGGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAg                                                                                                                                                                                         <  2:360711/251‑1 (MQ=255)
                                                                    ttGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAAt                                                                                                                                                                                                              <  1:94596/230‑1 (MQ=255)
                                                                      gCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGAt                                                                                                                                                                                       <  2:493212/250‑1 (MQ=255)
                                                                      gCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGAt                                                                                                                                                                                       <  1:253898/250‑1 (MQ=255)
                                                                      gCCGACGGGCGGGTGATTCTGGCGGACGGGCTGCAAGGCTACGGTCTGGTGG‑GGTGGGTGGAGCATGGTAAAGGAGACACGA‑GTCTATACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGGTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCAATGT‑CGAACTTCGCCGCCAGGGGCAGGCGGT‑AAATCCACAGCCGTGGTTGGG‑AAGATa                                                                                                                                                                                      <  2:146107/251‑1 (MQ=255)
                                                                       ccGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATaa                                                                                                                                                                                     <  1:193616/251‑1 (MQ=255)
                                                                          aCGGGCGGGTGATTCTGGCTGACGGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑tt                                                                                                                                                                                 <  2:229950/251‑1 (MQ=255)
                                                                                gggTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑CTTACTCTCTTT‑CGAAATTCTCCGCCCGGGTCAGGCGGG‑CCATTCCCCGCCCGGGTTGGG‑GAGATAAG‑gtttgttt                                                                                                                                                                           >  1:400396/1‑251 (MQ=255)
                                                                                  gTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTcc                                                                                                                                                                         <  2:400396/251‑1 (MQ=255)
                                                                                    gATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCAt                                                                                                                                                                       <  1:133061/251‑1 (MQ=255)
                                                                                    gATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCAt                                                                                                                                                                       <  1:221614/251‑1 (MQ=255)
                                                                                    gATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCTCTCTCTTT‑CGACATTCGACCCCCGGGGCAGGGGGT‑CCATCCACCACCGCGGTTGGG‑aggagtgattttttttccat                                                                                                                                                                        >  2:455906/1‑232 (MQ=255)
                                                                                    gATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGGGG‑TTGAGCAGGGTAAAGGCGACATGA‑GTCTTTACGGATATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCTCCGCCAGGGTCATGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTTTTTCCAt                                                                                                                                                                       <  2:511783/251‑1 (MQ=255)
                                                                                    gATTCGGGCTGGCTGGCGGCAGGGCTAGGGTCGGGTGGGGGTGG‑TTGAGCATGGTAAAGGCGTCATGA‑GGCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCAt                                                                                                                                                                       <  2:330987/251‑1 (MQ=255)
                                                                                         ggggtGACTGGCTGCAAGGCTGCGGTCTGTTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCCCTGGTGAGCGTTGGTTCGCAGGTGCGCGCGGGCCAGCCGATTGCACTGGGGGGCAGCAGTGGC‑GGTCAGGG‑TCGTCC‑TTCACTCTGTTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGGGGTTGGG‑AAGATAAG‑TTTGGTTTCCATTtcgt                                                                                                                                                                  <  2:590073/247‑1 (MQ=255)
                                                                                         gggCTGACTGGCTGCAAGGCGACGGTCTGGTGGTGGGGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTACGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTG‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTtcgt                                                                                                                                                                  <  2:282836/250‑1 (MQ=255)
                                                                                          ggCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTtc                                                                                                                                                                    <  1:7528/248‑1 (MQ=255)
                                                                                           gCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTtcgtcg                                                                                                                                                                <  2:127213/251‑1 (MQ=255)
                                                                                            cTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACTCTATTT‑CGGAATTTGCCGCCCGGGTCAGGCGGT‑CAATCCACAGCCGTGGGTGGG‑GAGATAAG‑TTTTGTTTCCATTtcttcgg                                                                                                                                                               >  1:236400/1‑250 (MQ=255)
                                                                                              gACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAAGCCACAGCCGTTGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTaa                                                                                                                                                             <  1:232566/251‑1 (MQ=255)
                                                                                                     tGCAAGGCTACGGTCTGGTGGTGGTGG‑ATGAGCATGGTAACGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGCCCGGG‑GCGGGC‑TTCACCCCATTT‑CGAAAATCGCCGCCCGGGGCAGGCGGT‑CCATCCACCGCCGTGGGTGGG‑GAGATAAG‑TTTTGTTTTCATTTTGTCGTAACGGTCtt                                                                                                                                                      >  1:519287/1‑251 (MQ=255)
                                                                                                      gCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATGAG‑TTTTGTTTCCATTTCGTCGTAACGTTCtt                                                                                                                                                      <  1:382702/250‑1 (MQ=255)
                                                                                                      gCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTg                                                                                                                                                     <  2:204149/251‑1 (MQ=255)
                                                                                                       cAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGc                                                                                                                                                    <  1:136202/251‑1 (MQ=255)
                                                                                                       cAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGAGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTGAGGG‑GCGGCC‑CTCACTCTATTT‑CGAAATTCGCCCCCAGGGTCAGGCGGTGCAATCC‑CCGCCGGGGGTGGG‑GAGATAAG‑TTTTGTTTTCCTTTCTTCGTAACGGTCtttc                                                                                                                                                    >  1:530108/1‑249 (MQ=255)
                                                                                                              aCGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑Gc                                                                                                                                             <  1:569796/250‑1 (MQ=255)
                                                                                                              aCGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCAGTTCGTCGTAACGTTCTTGCATTT‑Gcc                                                                                                                                            <  2:561452/251‑1 (MQ=255)
                                                                                                               cGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GGCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCg                                                                                                                                           <  1:117102/251‑1 (MQ=255)
                                                                                                                     ggtggtggtgg‑tTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGGTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGctct                                                                                                                                       <  1:51690/249‑1 (MQ=255)
                                                                                                                      gtggtggtgg‑tTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTGGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACGCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTGCTTGCATTT‑GCCGCTCTGtt                                                                                                                                    <  2:75966/251‑1 (MQ=255)
                                                                                                                       gggtggggg‑tTGAGCATGGTAAAGGCGACAGGA‑GTCTGTACGGCTATAATCAGATCGCGCTGGTGAGCGTGGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACGGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TGTTGTGTCCATTTCGTCGGAACGTTCTTGCATTG‑GCCGCTCTGTTg                                                                                                                                   <  2:606090/250‑1 (MQ=255)
                                                                                                                        ggtggttg‑tTGATCATGGTAAAGGCG‑CCTGAAGTCTTTACGGCTATAGTCAGAGCGCACTGGTGAGCGTTGTTGCGCAGGTTCGCGCTGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGGCAGGG‑TTGGCC‑TTCACTCTATTT‑CGAAAGTCGCCGCCAGGGTCAGGCGTT‑CAATCCACAGCCGTGGTTGGG‑AAGAAAAG‑TTTTGTTTCCAGGTCTTCGTAACGTGCTTTCATGT‑GCCGTTCTGTTg                                                                                                                                   <  2:412417/250‑1 (MQ=255)
                                                                                                                         gtggtgg‑tTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCCGGGTCAGGCGGT‑CAATCCACAGCCGTGGGTGGG‑GAGATAAG‑TTTTGTTTTCATTTTGTCGTAACGGTCTTGCATTT‑GCCGCTCTGtttgg                                                                                                                                 >  1:268275/1‑248 (MQ=255)
                                                                                                                          tggtgg‑tTGAGCATGGTACAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GTGGCC‑TTCCCTCGATTT‑CCAAATTTGGCGCCCGGGTCAGGCGGT‑CTATCCACAACCGTGGGTGGG‑CAGGTTAG‑ATTTGGTTTCATTTCGTCGTCACGTTTTTTCATTT‑GTCCCTCTGTTgggg                                                                                                                                >  1:152594/1‑249 (MQ=255)
                                                                                                                            gtgg‑tTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TAGGCC‑CTTACTCTCTTT‑CGAAATTCGCCGCCAGGGTCAGGGGGG‑CCATCCACAGCCGGGGTTGGG‑CAGATAAG‑GTTTGTTTCCCTTTCGTCGTAACGTTCTTTCATTT‑GTCGCTCTTTTGGCGct                                                                                                                              >  1:399795/1‑251 (MQ=255)
                                                                                                                              gg‑tTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCCGGG‑GCGGAC‑TTCACTCTATTT‑TGAAATTCGCCCCCCGGGGCAGGCGGT‑CAAGCCCCAGCCGCGGTTGGG‑GAGATAAG‑TTTTTTTTCCCTTTTGTCGTAAAGTTCTTGTGTTT‑GCCGCTCTGTTGGCGctct                                                                                                                            >  2:323647/1‑251 (MQ=255)
                                                                                                                                   gAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGAGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCACGC‑GCGGCC‑TTCCCCCCATTT‑CGGCATTCGCCGCCAGGGGCCGGCGGG‑CAATCCACCACCGTGGGTGGG‑GAGATAAG‑ATTTTTTTCCATTTTTTCGGAACGTTTTTGCATTT‑TTCGCCCTGTTTGCGCTctcctc                                                                                                                        >  1:565620/1‑251 (MQ=255)
                                                                                                                                       agggTAAAGGCGAAATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTccccc                                                                                                                    <  2:250654/249‑1 (MQ=255)
                                                                                                                                         ggTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGt                                                                                                                  <  2:466293/251‑1 (MQ=255)
                                                                                                                                                 cGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACTCTTTTT‑CGGAAATCGCCGCCAGGGTCAGGCGGG‑GAATCCACAGCCGGGGTTGGGGAGGTTAGG‑TTTTTTTTCCATT‑CCTCGTAACGTTCTTGCATTT‑TACGCTCTGTTTGCGGCCTCCTccccccgtcttgcct                                                                                                          >  2:164468/1‑241 (MQ=255)
                                                                                                                                                   aCATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑CTCCCTCTCTTT‑CGGAATTTGCCGCCAGGGGCAGGCGGG‑CAAACCCCCGCCGTGGTTGGG‑GAGAATAG‑TTTTTTTTCCATTTCGTCGTAACGTTTTTTCCTTT‑TCCCCTCTTTTTGGGCCCTCCTCCCCCgccccttctggc                                                                                                        >  2:163804/1‑240 (MQ=255)
                                                                                                                                                     aTGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGATCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGGCCGGG‑GCGGCC‑CTCACTCTATTT‑CGTAATTCGCCGCCAGGGGCAGGCGGT‑CAACTCACAGACGGGGGTGGG‑AAGGAAAG‑TTTTGTTTCCATTTCGTCCTAACGTTCTTGCATTT‑TCCGCTCTGTTGGGGCCCTCCTccccccctcttgctggga                                                                                                       >  1:571808/1‑237 (MQ=255)
                                                                                                                                                        a‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACTCCTTTT‑TGGAATTTGCCGCCAGGGGCAGGCGGT‑CAATTCACAGCCGTGGTTGGG‑GAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCCCCCTGTTGGCGCTCTCCTCCCCCGCACTTGCTGGCAAACt                                                                                                   >  1:569386/1‑251 (MQ=255)
                                                                                                                                                          gTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACtt                                                                                                  <  1:544088/251‑1 (MQ=255)
                                                                                                                                                              ttACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTg                                                                                                 <  2:132120/248‑1 (MQ=255)
                                                                                                                                                                aCGGCTATAATCAGAGCGCACTGGTGAGCGTGGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTAGTT‑CGAAATTCGCCGCCAGTGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTACCGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCAt                                                                                             <  2:214843/250‑1 (MQ=255)
                                                                                                                                                                      atgaTCAGAGCGCACTGGTGGGGGGTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTTGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCGCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCAt                                                                                       <  2:179514/247‑1 (MQ=255)
                                                                                                                                                                        aaTCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATtga                                                                                    <  1:327083/251‑1 (MQ=255)
                                                                                                                                                                           cAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTTTTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAACCGTGGGTGGG‑AAGATAAG‑TTTTGTTTTCATTTCGTCGTACAGTTCTTGCCTTT‑GCCGCTCTGTTTGCGGTCTCCTCCCCCGTACTTTTTGGCCAACTTGCCAttctcaatgatg                                                                                  >  1:510197/1‑239 (MQ=255)
                                                                                                                                                                             gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACTCTATTT‑CGAAATTCTCCGCCCGGGGCCGGCGGG‑CAATCCACCGCCGCGGTTGGG‑GAGGTAAG‑TTTTGTTTCCATTTTTTCGTAACGTTCTTGCATTT‑GCCGCTCTTTTGGGGCCCTCCTCCCCCGTACTTTCTGGCCAACTTGCCCTCGTCCTTGTTGAtt                                                                               >  2:140488/1‑251 (MQ=255)
                                                                                                                                                                              agCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGAttt                                                                              <  2:421331/251‑1 (MQ=255)
                                                                                                                                                                                cgcACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGGGTCG‑CC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAAGCGGT‑CAATCCAAAGCCCGGGTTGGG‑AAGATAAG‑GTTTGTTTCCATTTTGGCGTAAAGGTCTTGCATTT‑TCCGCTCTGTTTGCGCTCTCCTCCCCCGTACTTGCTTGCAAACTTGCCATCCTCATTGATGCttttt                                                                            >  1:456991/1‑250 (MQ=255)
                                                                                                                                                                                 gcACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACTCTATTT‑TGCAATTTGCCGCCCGGGGCAGGAGGG‑CAATCCACAGCCGTGGTTGGG‑GAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCCTCGTCATTGATGAtttttg                                                                           >  1:224453/1‑249 (MQ=255)
                                                                                                                                                                                 gcACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGACGATTGCACTGGTGGGCAGCAGTGGC‑GGTCCGGG‑TCGGCC‑TTCACCCTCTTT‑CGACATTTGGCGCCACGGTCAAGCGGT‑CCATCCCCCGCCCAGGGTGGG‑GGGATAAG‑GTTTGTTTCCATTTCATCGTAACGTTCTTGCATTT‑TCCGCCATTTTGGCGCTCTCCTCCCCCCTACTTGCTGGCAAACTTGCCCTCGTCCTTGATTATTTTgg                                                                           >  1:303500/1‑251 (MQ=255)
                                                                                                                                                                                   ccTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGt                                                                         <  1:610282/250‑1 (MQ=255)
                                                                                                                                                                                   aCTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGt                                                                         <  2:224453/251‑1 (MQ=255)
                                                                                                                                                                                   aCTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACTTTATTT‑CGCAATTCGCCGCCCGGGGCCGGCGGG‑CAATCCACACCCGCGGGTGGG‑GAGATAAG‑TTTTTTTTCCATTTCCTCGTTAAGTTCTTGCATTT‑GCCCCCCTTTTGGGGCTCTCCTCCCCCCGACTTGCTTGCCAACTTGCCCTCGGCCTTGATGCtttttggg                                                                         >  1:378269/1‑247 (MQ=255)
                                                                                                                                                                                     tGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGACCGGG‑GCGGGC‑TTCACTCTCTTT‑TGAAACTCCCCGCCAGGGGCCGGCGGT‑CAATCCACAGCCGAGGGTGGG‑GAGATACG‑TTTTGTTTTCCTTTCGTCGGAAAGTTCTTCCATTT‑GCCGCCCTGTTGGCCGTCTCCTCCCCCGTTCTTGCTGGCAAACTTGCCATAGTCCATGGTGATTTTGGGttt                                                                       >  1:562357/1‑249 (MQ=255)
                                                                                                                                                                                     gggggAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTAt                                                                       <  2:133175/247‑1 (MQ=255)
                                                                                                                                                                                      ggTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATc                                                                      <  1:163804/251‑1 (MQ=255)
                                                                                                                                                                                      ggTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CCAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTAt                                                                       <  1:52498/250‑1 (MQ=255)
                                                                                                                                                                                         gAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATCGcc                                                                   <  2:530108/251‑1 (MQ=255)
                                                                                                                                                                                         gAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTGGGGTATCGc                                                                    <  1:445981/250‑1 (MQ=255)
                                                                                                                                                                                          aGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑TTCACTCTCTTT‑TGCAATTCGCCGCCCGGGTCCGGCGGC‑CCATCCCCAACCGTGGGTGGG‑GAGATAAG‑TTTTGTTTTCATTTTGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGGTCTCCTCCCCCGTCCTTTCTGGGAAACTTGCCCACGTCCTTGATGATTTTTGGGATCGccc                                                                  >  1:33921/1‑251 (MQ=255)
                                                                                                                                                                                                                 gggCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTTAGGG‑GCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCCGGGGCCGGCGGG‑CAATCCCCAGCCGTGGGTGGG‑GAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTTGCGCTCTCCTCCCCCGTACTTGCTGGCAAAATTGCCCTCGTCATTGATTATTTTTGGTATCTcccccccacagaaaaccaagggctgg                                           >  1:479445/1‑228 (MQ=255)
                                                                                                                                                                                                                 gggCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTgg                                           <  1:323647/251‑1 (MQ=255)
                                                                                                                                                                                                                  ggCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATCCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTGGc                                          <  2:190533/251‑1 (MQ=255)
                                                                                                                                                                                                                   gCCAGCCGATTGCACTGGGGGGCAGGAGTGGC‑GGTCAGGG‑TCGGCC‑GGCACGCTATTT‑CGAAAGTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGAGTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTGGCg                                         <  2:303996/251‑1 (MQ=255)
                                                                                                                                                                                                                      aGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTTAGGG‑TCGGCC‑CTCACTCTATTT‑TGAAAATCGCCGCCCGGGTGCGGCGGG‑CCATCCACAGCCGTGGGTGGG‑GAGAGTAG‑TTTTGTTTTCCTTTCCTCGTAACGTTCTTGCATTT‑TCCGCTCTGTTGGCGCCCCCCTCCCCCGGACTTGCTGGCAAACTTGCCATCGGCATTGATGTTTTTTGGTATTGCCCGCCCAACGAAAAACAGGGGCTGGGGATg                                      >  2:165028/1‑251 (MQ=255)
                                                                                                                                                                                                                          gATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGGC‑TTCACTATATTT‑TGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGGCGGGGTTGGG‑GAGATAAG‑TTTTGTTTCCCTTTCGTCGGAAACTTCCTGCATTTTGCC‑CTCTTTTGGCGCTCTCCCCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTTGGTAACGCCCGCACCAAGAAAACCAGGGGCTGGCGGTGCCt                                   >  1:282613/1‑250 (MQ=255)
                                                                                                                                                                                                                          gATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGGC‑TTCCCTCTATTT‑CGGCAATCGCCCCCCGGGGCCGGCGGT‑CAATCCCCCGCCGGGGGTGGG‑GCGGTAAGGTTTTTTTTCC‑TTTTGTCGTAACGTTCTTGCCTTT‑TTCGCTCTGGTTGCGCTCTCCTCCCCCCTACTTGCTGGCCAACTTGCCATCCTCCTTGTTGATTTTTGGTCTCGCCCGCCCAACGAAAACCAGGTGCTGGGGGTGCCtt                                  >  2:379445/1‑251 (MQ=255)
                                                                                                                                                                                                                           aTTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑GCGGCC‑CTCACTCTATTT‑TGAAAATCGCCGCCAGGGGAAGGCGGT‑CAATTCACAGCCGGGGGTGGG‑GAGGTAAG‑GTTTGTTTCCATTTCGTCGTAAAGTTTTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACCTGTCATCGTCACTGGTGATTTTGGGTATTGCCCGCGCCACGAAAACCAgggggggggggtgccttc                                 >  2:425817/1‑235 (MQ=255)
                                                                                                                                                                                                                                         cagcagTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTGGCGATGCCTTCCGCTATCTCCGTCg                   <  2:268275/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    ggTCAGGG‑TCGTCC‑TTCACTCTATTT‑CGAAATTCGCCCCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTGGCGATGCCTTCCGCTATCTCCGTCGCTGTATTAcc         <  2:509958/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     gTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTGGCGATGCCTTCCGCTATCTCCGTCGCTGTATTAccc        <  2:39449/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     gTCAGGG‑TCGGCC‑TTCACTCTAGTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATGT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTGGCGATGCCGTCCGCTATCTCCGTCGCTGGATTAccc        <  2:69409/251‑1 (MQ=255)
                                                                                                                                                                                                                                                          gg‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTGGCGATGCCTTCCGCTATCTCCGTCGCTGTATTACCCGATTc   >  1:406170/1‑251 (MQ=255)
                                                                                                                                                                                                                                                           g‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGAGTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTGGCGATGCCTTCCGCTATCTCCGTCGCTGTATTACCCGATTCa  <  2:244828/251‑1 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                               
GCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTACTGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGA‑GTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGC‑GGTCAGGG‑TCGGCC‑TTCACTCTATTT‑CGAAATTCGCCGCCAGGGTCAGGCGGT‑CAATCCACAGCCGTGGTTGGG‑AAGATAAG‑TTTTGTTTCCATTTCGTCGTAACGTTCTTGCATTT‑GCCGCTCTGTTGGCGCTCTCCTCCCCCGTACTTGCTGGCAAACTTGCCATCGTCATTGATGATTTTGGGTATCGCCCGCACAACGAAAACCAGGTGCTGGCGATGCCTTCCGCTATCTCCGTCGCTGTATTACCCGATTCA  >  NC_000913/3787776‑3788274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑