| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,622,250 | 0 | A | G | 35.1% | 91.6 / 17.6 | 57 | E161G (GAG→GGG) | yjjJ | toxin YjjJ |
| Reads supporting (aligned to +/- strand): ref base A (8/29); new base G (20/0); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.57e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTG‑TTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGAC > NC_000913/4622013‑4622488 | gCTGGCCGCAGGGACGTTGTCTGGAAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGGCg‑gggggggggaatatacg > 1:491005/1‑242 (MQ=255) ccGCAGGGTAGTTGGCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGGTGGTTTGCCCTGGAATTTGACCGGTCTCCGACCGCAGGGCTTTTTCGGGCTCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTTACTGATGATATACGTCTCTGGCTGGAAGAAGATGTGCTCTACGCCCTGACCGCATTTAACGGTGAATATACTGGCGGTT‑GG‑TGGGGCG‑GGGAGGGGGATTAttcaagg > 2:382110/1‑243 (MQ=255) gggAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTTGGGGG‑GGGGGGGGAAtattaggggtggggt > 1:401329/1‑236 (MQ=255) gTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGGGG‑GGGAGGGGGATGATCAGAGA‑AGG‑Attgctttcca > 1:84080/1‑242 (MQ=255) ggTAACAGGCGCAGATGGCGACGAACAGTGGTTTGGTGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCac‑aac < 1:40509/248‑1 (MQ=255) ggTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCac‑aaca < 2:453671/249‑1 (MQ=255) ggTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑Aacac < 1:217301/250‑1 (MQ=255) ggTAACAGGCGCAGATGGCGACGAACAGTGGTTGGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCac‑aac < 2:484570/248‑1 (MQ=255) ggCGCAGATGGCGACGAACAGTTGTGTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑a < 2:425406/251‑1 (MQ=255) cAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACTCCCTGCGG‑AAAtt < 2:411696/251‑1 (MQ=255) cAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCGGACCGTATTTAACGGTGAATATACTGGGGGGT‑GG‑TTGGGCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAAtt < 1:382110/251‑1 (MQ=255) aGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑GTGGTTG‑GGGGGGGGGATTGTTAGCGA‑TGG‑ATTATTGCAC‑CACAccccgccgggaatt > 2:568483/1‑240 (MQ=255) gATGGCTACGAACAGTGGTTTGATGGGTTGCCCTGTTATTTGACCGATCTCCGACCGCAGGGCTTGTTAGGGCGCGCGTGGGGCAGGAAGGTAACCGCGCAACTGAATCTGACTGATGATATACGACTCTGGCAGGAAGAAGATGTGCTCTACGCCCGGACCGTATTTAATGGTGAATATACTGGCGTTT‑CG‑TTAGTCG‑GGGAGGCGAACGGTCAGCGA‑GGT‑ATTACAGCTC‑ATCGCCCTGCGG‑AAATTcc < 2:351762/251‑1 (MQ=255) gATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTcc < 1:486919/251‑1 (MQ=255) ggggTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTGTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCGCTGGCAGGAAGAAGATGTGCTCGACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTACTCTGGAT‑CAAAAAc < 2:125285/249‑1 (MQ=255) tGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGCT‑GG‑TTGGGTG‑GGGGGGGGGATTATCCGCGAGTGG‑TTTAC‑GTAC‑AACACCCCGCGGGAAATTTC‑CTGGGT‑CAAAAACTC‑Accc > 2:188646/1‑251 (MQ=255) ggTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACGGCAC‑AACACTCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTa < 1:248532/251‑1 (MQ=255) cccTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTTCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACa < 2:44273/251‑1 (MQ=255) cTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑GTGGGTG‑GGGGGGGGAATTTTCAGCGA‑TGG‑GTTACTGCAC‑AAAACCCCGCGGGAAATTC‑TCTGGGT‑CAAAAACTC‑ACCC‑CTTACGAACAGc > 1:155185/1‑251 (MQ=255) ggTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCt < 2:214912/250‑1 (MQ=255) aTTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑c < 1:437206/250‑1 (MQ=255) tGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGGCG‑GGGGGGGGAATTATCAGCGA‑TGG‑GTTTCTGCAC‑AACACCCCGCGG‑GAATTTCTCTGGAT‑CAAAAACTC‑ACCC‑ACTACGAACAGCTGG‑ccagg > 1:202619/1‑247 (MQ=255) cGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑GTGGTCG‑GGGGGGGGGAATATCAGCGA‑TGG‑GTTTCTGCAC‑AACACCCTGCGG‑AAAATACCCTGGGT‑TAAAAAATC‑AACC‑CTTACGCAAAGCTGG‑ccagataat > 1:403782/1‑243 (MQ=255) gATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑GTGGTCG‑GGGGGGGGGATTTTTAGCGA‑TGG‑ATTACTGCAC‑AAAAACCTGAGG‑AAAATCCTCTGGAT‑CAAAAAATC‑ACCC‑CATACGAACAGccgggaaaggatgc > 1:54263/1‑238 (MQ=255) gACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCa < 1:306944/251‑1 (MQ=255) cGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑GTGGTCG‑GGGGGGGGGATTTTCAGCGG‑TGG‑GTTACTGCACCAAC‑CCCTGCGG‑CAATTCCCCCGGTT‑CAAAAAACC‑ACCC‑ACTACGCACAACTGG‑CAAggggtgccatggccggg > 2:300530/1‑235 (MQ=255) cAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTTGG‑TGGGT‑G‑GGGGGGGGGATTATTAGCGG‑AGG‑GTTTCTGCAC‑AAAACCCTGCGGGAAATACC‑CTGGAT‑TAAAAAATCCACCC‑‑CTAAGAACAGCTGG‑CAAAGGATGC‑CCTGGccgggg > 1:81940/1‑245 (MQ=255) ggCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAAt < 2:202619/251‑1 (MQ=255) gTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑GTGGTCG‑GGGGGGGGGATTTTCTGCGA‑TGG‑ATTACTTCAC‑CACACCCTGCGG‑AAAATCCTCTTGGT‑CAAAAACTC‑CCCCCTTTAC‑AACCGCTGG‑CAAGGGGTGC‑ACCGGCC‑GGAGGAAAtttggggttttctctgg > 2:414040/1‑236 (MQ=255) cAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGgcggcg < 2:155185/251‑1 (MQ=255) aGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGCATATACTGGCGGTT‑TG‑TTGGTCG‑GGGGGGGGAATTATTAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑GAATTCCTCTTGGT‑CAAAAACTC‑CCCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGGG‑AAATTGTGGGGTCTT‑CTGCGGGGGGCGagc > 1:314577/1‑249 (MQ=255) gTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGagcagc < 2:486956/251‑1 (MQ=255) tAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGcc < 1:71400/250‑1 (MQ=255) gaGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATGATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTACTCTGGAT‑CAAAAACTC‑ACCC‑AGTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑TGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGcc < 1:555491/249‑1 (MQ=255) cAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTAc < 1:414119/251‑1 (MQ=255) aaTCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑GTGGTAG‑GGGAGGGGGATTATTAGCGA‑TGG‑GTTACTGCAC‑AACACCCTGCGG‑GAAATCCTCTGGGT‑CAAAAACTC‑ACCC‑ATTAAGAAAAGCTGG‑GAAGAGAAGC‑ACTGGCA‑GGGG‑AAAATTTGGGGTCTT‑TTGCGGGCGGCGCGCAGCC‑AAAAATTACctcac > 1:273008/1‑247 (MQ=255) gatgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTATGCACAAAc < 1:131921/251‑1 (MQ=255) atgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGGCG‑GGGGGGGGGAATATCAGCGG‑TGG‑ATTACTGCAC‑AACACCCCGCGG‑GAATTCCTCTGGGT‑CAAAAAATC‑ACCC‑ATTACCAACAACTGG‑CAAGGGATGC‑ACTGGCA‑GGAGGAAATT‑TGGGTTCTTTCT‑CGGGGGGGGAGGAGCCCAAAATTTAC‑TAATATGCACaaa > 1:508486/1‑249 (MQ=255) atgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTAAGCACTGACCGTCTTTAACGGTGAATATACTGGCGGTT‑GG‑GTGGGCG‑GAGAGGGGGATTATCAGGGC‑TGG‑TTTACTCCAA‑CACACCCTGCGG‑GAATTCCTCTGGGG‑CAAAAACTC‑ACCC‑CTTACGAACAGCTGG‑AAACAGAAGC‑ACTGGCA‑GGGG‑AAACTTTGGGGTTTT‑CTGCGGGGGGGGAGCAGCC‑CAAAATTACCTTCCTTGCACaaa > 1:30554/1‑249 (MQ=255) aCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGGGGGGGAATATCAGCGA‑TGG‑GTTACTGCAC‑AACACCCTGCGG‑GAATTCCTCTGGGT‑CAAAAACTC‑ACCC‑CTTACGAACAGCTGG‑CCAGGGATGC‑CCTGGCA‑GGGG‑GAATTTTGGGGTCTT‑CTGCGGGGGGGGGGCAGCCCAAAATTT‑CCTACTATTCACAAACGCCggc > 1:492571/1‑247 (MQ=255) cGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACC‑‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTATGCACAAACGCCGTCAGGc < 1:595745/251‑1 (MQ=255) gTCTCTGGAAGGAAGAAGATGCGCCCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGAT‑GG‑GTAGTCG‑GGGAGGGGGATTTTCAGCGG‑TGG‑GTTTCTGCAC‑AACACCCCGCGG‑AAAATACTCCGGGT‑CAAAAAACC‑AACC‑ACTACGACCACCTGG‑CAAGTGATGCCAC‑GGCA‑GGAG‑AAAATGTGGGGTTTT‑CTGCGGGCGGGGAGCAGCCCAAAATTT‑CCTACTATTCACAAACGCCGTccagc > 1:188068/1‑247 (MQ=255) ctctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑AG‑TTGGGGGAGGGGGGGGATTTATCAG‑GG‑TGGCATT‑CTTCAC‑ACCACCACGCCG‑AAAATTCTCTGGAT‑CCAAAAATC‑ACCC‑ATTCAGAACAGGTGGACAA‑TGATGC‑CCTGGCACGGGC‑AAATT‑TTGGTTCTT‑ATTCGGGCGGCGGGCAGCCCAAAATTT‑Cctacttttaccaaacgcccgcaggcc > 1:54854/1‑229 (MQ=255) cgctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTATGCACAAACGCCGTCAGGCaa < 2:483775/249‑1 (MQ=255) tctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGGCG‑GGGGGGGGAAATATCAGCGA‑TGG‑ATTACTGCAC‑AAAACCCTGCGGGAAATTC‑TCTGGGT‑CAAAAACTC‑ACCC‑ATTACGAAAAGCTGG‑CAAATGATGC‑ACTGGCC‑GGGG‑AAATTGTGGGGTCTT‑TTTCGGGGGGGGGGGAGCC‑CAAAATTACATACTATGGACAAACGcccgcagggaa > 1:142527/1‑241 (MQ=255) ggCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑TG‑TTGGGCG‑GGGGGGGGAAATATCAGCGA‑TGG‑ATTAATGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAAATC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACCGGGA‑GGAGGAAATT‑TGGGGTTTT‑CTTTGGGGGGCGCGGCGCC‑AAAAATTTCCTAATTTGCACAAACGCCGTCAGGCAATaa > 1:601552/1‑250 (MQ=255) aagaagATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACAtgtg < 1:568483/251‑1 (MQ=255) aagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑TG‑GTGGTCG‑GGGGGGGGGATTTTTAACGA‑TGG‑ATTTCTGCAC‑AAAACCCTGCGG‑GAATTTCTTTGGGT‑CAAAAAATC‑ACCC‑ATTACGCAAAACCGG‑GAAGTGATGG‑ACCGGCA‑GGGGGAAATT‑TGGGGTTTT‑TTGCGGGGGGGGGGGAGCCCAAAATTTAC‑TACTATGCACCAAAGCCGCCAGGGCATAAACATATG‑ttt > 2:438054/1‑250 (MQ=255) gtgCGCTACGCCCGGACCGTATTTAACGGTGAATATACGGGCGGTT‑GG‑TGGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACAGCAC‑AACACACTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTCCGAACAGCTGG‑CAAGTGATGC‑GCTGGCA‑GTAG‑AAATTGTGGGTGCTT‑CGGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTATGCTCAAACGCCGTCAGGCGATACACCTGTG‑TGGGTGaa < 2:395068/251‑1 (MQ=255) ctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑TG‑GTGGTTG‑GGGGGGGGAATTTTCAGCGA‑TGG‑GTTACTGCAC‑AACACACAGCGG‑AAATTCCTCTGGATTCAAAAAC‑C‑ACCC‑ATTACGAACAGATGG‑CAAGTGATGC‑ACTGGCA‑GGGG‑AAATTGTGGGTTCTT‑CTGCGGGGGGGGGGCAGCC‑AAAATTTAACTAATATTAAAAAACGCCGTCCGGCCATAAACATGTG‑TTTGTGAAAtttt > 1:580791/1‑249 (MQ=255) ctACGCCATGACCGTATTTAACGGTGACTATACTGGCGGTT‑GG‑TTGGTCG‑GGGGGGGGAATTATTAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑GAATTTCTCTGGGT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGGGGTGC‑ACTGGCA‑GGGG‑AAATTGTGGGGTCTT‑CTGCGGGCGGCGGGCAGCC‑CAAATTTTACTACTATGCACAAAAGCCGTCAGGGAATAAACATGTG‑TTGGTGAAAttt > 1:460331/1‑249 (MQ=255) gACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGGCG‑GGGGGGGGGATTAACAGCGA‑TGG‑ATTACTGCAC‑AACACCCTCCGG‑AAATTTCTCTGGAT‑CCAAAACTC‑ACCC‑ATTAAGAACCGCTGG‑GAAGGGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGGTCTT‑CTGCGGGCGGCGGGGAGCC‑AAAAATTACCTACTATGCACCAAAGCCGCCAGGGAAAAAAAATGTGGTTGG‑GAAATTTACCCTACcac > 1:193991/1‑251 (MQ=255) cGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTG‑TTGGTGAAATTCACCGTACCACAGc < 1:133641/251‑1 (MQ=255) tAACGGTGAATATACTGGCTGTT‑GG‑GTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTAAGCACAAACGCCGTCAGGCAATAAACATGTG‑TTGGTGAAATTCACCGTACCACAGCAAAcc < 2:560825/250‑1 (MQ=255) taCTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAAtt < 1:398415/133‑1 (MQ=255) gCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGCTGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCCGCC‑AAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGGG‑TTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCACCGTTgg > 2:316743/1‑251 (MQ=255) gg‑ttggtCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTG‑TTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGAc > 2:342328/1‑251 (MQ=255) gg‑ttggtCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTG‑TTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGAc > 1:414569/1‑251 (MQ=255) | GCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GG‑TTGGTCG‑GGGAGGGGAATTATCAGCGA‑TGG‑ATTACTGCAC‑AACACCCTGCGG‑AAATTCCTCTGGAT‑CAAAAACTC‑ACCC‑ATTACGAACAGCTGG‑CAAGTGATGC‑ACTGGCA‑GGAG‑AAATTGTGGGTTCTT‑CTGCGGGCGGCGAGCAGCC‑AAAATTTACCTACTATGCACAAACGCCGTCAGGCAATAAACATGTG‑TTGGTGAAATTCACCGTACCACAGCAAACCGCGGTCAGCCAACGTTGGGGTGAC > NC_000913/4622013‑4622488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |