| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,909,602 | 0 | G | A | 59.6% | 34.1 / 39.6 | 49 | A22V (GCA→GTA) | pyrG | CTP synthetase |
| Reads supporting (aligned to +/- strand): ref base G (8/11); new base A (15/13); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAAC‑GCTCGTAGTGCCCCAGGTCCAGGT‑CGGTTTCAGCGCCGTCTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAGCGCACTATTGAAATCAGGAGTGAGAAAATAGCCCCTTTGGGGTAAATGTTTTTGACGCAAATCAA > NC_000913/2909356‑2909842 | cgTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAAC‑GCTCGTAGTGCCCCAGGTCCAGGT‑CGGTTTCAGCGTCGTCTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGc > 1:126891/1‑251 (MQ=255) cAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGTTCATTTTGGTACGAATGAAAC‑GCTCGTAGTGCCCCAGGTCCAGGT‑CTGTTGCAGCGCCGTCTTCAGTAACGAACGC‑TTCCCCGTGGGGGATGGGGCTCACAGTGCCTGGATCGACTTTGATGGAC‑GGATCCAGTTTCATGATGGTCACATTGAGATCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTAc < 1:574439/251‑1 (MQ=255) gAACGTGAGAGTAGATCCGACCCGTGGTGAAGTTGTTGCGTCGGCTCATTTTGGTACGAATGGAGC‑GCTCGTAGGTCCCCAGGGCCAGGT‑CGGTTTCAGCGCAGTCTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGACGCATAGTACTTGGATCGACGTTTATGTAC‑GGATCCAGTTTCATGATGGGCACATTTAGGCCACGGGCTTCAAGAACGTCGACGAGTGAGAGTGCTGCAATGCCTTTAccc < 2:511147/251‑1 (MQ=255) cAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAAC‑GCTCGTAGTGCCCCAGGTCCAGGT‑CGGTTTCAGCGCCGTCTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCagaga < 1:160298/250‑1 (MQ=255) cAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAAC‑GCTCGTAGTGCCCCAGGTCCAGGT‑CGGTTTCAGCGCCGTCTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCagaga < 2:402055/250‑1 (MQ=255) gggtgAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAAC‑GCTCGTAGTGCCCAAGGTCCAGGT‑GGGTTTCAGCGCCGTCTTCAGTAACGAACCC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCGGGATCGACGTTGATGTAC‑GGATCCATTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGCCCCCGCCGGTc < 1:366145/250‑1 (MQ=255) gcggcgGCTCATTTTGGTACGAATGAACC‑GCTCGTAGTGCCCCAGGGCCAGGT‑CGGTTGCAGCCCCGTCTTCAGTAACGAACGC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATTTAC‑GGATCCATTTTCATGGGGGTCACATTGAGTCCACGTGCTTCAAGATTGGCTGCGAGGGAGGCTACTGTAGTGCGTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAg < 1:44152/250‑1 (MQ=255) cggcgcttCATTTTGGTACGAATGAAAC‑GCTCGTAGTGCCCCAGGTCCAGGT‑CGGTTTCAGCGCCGTCTTCAGTAACGAACCC‑TTCCCCGTGTTGGATTGGGCTCATAGTCCCTGAATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAAAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGtt < 1:137870/244‑1 (MQ=255) ggcggcTAATTTTGTTACAAATGAAAC‑CCTCGTAGTGCCCC‑GGTCCCGGTTGGGTTTAAGCGCCTTCTTCAGTAACGAACCC‑TCCCCCGTGTGGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GAATCCATTTTCAGGATGTTCACATTGAGGCCCCGGGCTTCAAGAAGGGCTGCGAGGGAGGCTACGTAAAGCCCTTTCCCCAGAGAGAATACGACCCCGCCGGTCACAAAAATATAGTTc < 2:126891/251‑1 (MQ=255) gcTCATTTTGGTACGAA‑GAAACCGCGCGTAGTGCCCCAGGTCCAGGT‑CGGTTTCAGCGCCGTCTTCAGAAACGAACCC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCGGGATCGCCGTTGATGTAC‑GGATCCAGTTTCAGGATGGTCACATGGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGAAATGCTTTTACCCAGAGAGGATACGCCCCCGCCGGTCACAAAAATATAGTTCGTTg < 2:268621/251‑1 (MQ=255) gAATGAAAC‑GCTCGTAGTGCCCCAGGTCCAGGT‑CGGTTTCAGCGCCGTCTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTg > 1:71163/1‑251 (MQ=255) cc‑GCTCGTAGTGCCCCAGGTCCAGGT‑CGGTTTCAGCGCCGTCTTCAGTAACAAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTCCCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGCCCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGtt < 1:596681/250‑1 (MQ=255) ccgcgcGTAGTGCCCGAGGCCCAGGT‑CGGTTTCCGCGCCGTCTTCGTTAACGAACGC‑GTCCCCGGGTGGGATTGGGCTCAAAGTACCTGGATCGAGGTGGA‑GTCCCGGATCCAGTTTCATGATGGTCACATTGAGGCCGCGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGAAATGCCTTTCCCCAGATAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGtt < 2:165051/246‑1 (MQ=255) gTAGTGCCCCAGGCCCAGGT‑CGGTTTCAGCGCCGTCTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCccgcc < 2:112319/201‑1 (MQ=255) aGTGCCCCAGGTCCAGGG‑CGGTTTCAGCGCCGTCTTCAGTAACTAACAC‑TTCCCCGTGTGGGATTGGGCTCATAGTACCTGGAGCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCGGCGGCAATGCCTTTACCCAGAGAGGATCCAACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGa < 1:60674/250‑1 (MQ=255) gggtccaggt‑cGGTTTCAGCCCCGTCTTCAGTACCGAACAC‑TTCCCCGGGGTGGATGGGGCTAATAGTACCTGGATCGGCGTGGATGTAC‑GGATCCTGTTTCATAAGGGTAACATGGAGGCCACGGGCTACAAGAATGGCTGGGAGGGGGGCTACGGAAATGCCTTTACCAAGAGAGGAGACGACCCCGCCGGTCACAAAAATATAGGTCGTTGTCATGCTGAACCTGAGAAGTTAGGTGGAAAAGACGAt < 2:391766/250‑1 (MQ=255) aggtccaggt‑cGGTTTCAGCGCCGTCTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCACAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGAt > 1:591558/1‑251 (MQ=255) gtccaggt‑cGGTTTCAGCGCCGTCTTCAGTAACGAACAC‑TTACCCGTGTTGGATTGGGCTCATAGTAACTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATgg > 1:407549/1‑251 (MQ=255) ggt‑cGGTTTCAGCGCCGGCGTCAGTAACGACCAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGTTGAACCTGAGAAGTTAGGTTGCAAAGACGATGGAAtg > 2:415534/1‑249 (MQ=255) ggggTTTAAGCGCCGTCTTCAGTAACGAACCC‑TTCCCCGTGTTGGATTGGTCTCATAGTACCTGGTTCGACGTTGAGGTGC‑GGAGCCAGTTTCAGGAGGGTCACATTGGGGCCACGGGCTCCAAGAAGGGTGGCGGGGGGGGCTACGGAAAGGCCGTTCCCCAGAGAGGTTGCCACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAAcc < 2:400607/249‑1 (MQ=255) ggTTTCAGCGCCGTCTTCAGTAACGAACAC‑TTCCCCGTGTCGGATTGGGCCCCTAGTACCTGGATCGACGCTGATGTAC‑GGATCCCGTTTCATGATGGTCACCTTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCACTGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAACAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCa > 1:576025/1‑250 (MQ=255) gttccggccccctCTTCAGTAACGA‑CACCTCCCCCGGTTTGGATTGGGCTAAAAGTACCTGGTCCGGCGTTGATGTAC‑GGATCCAGTTTAATGATGGTCACATGGAGGCCCCGGGCTTCAAGAAGGGCTGCGAGGGAGGCTACGGAAATGCTTTTCCCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAg < 2:9338/239‑1 (MQ=255) cAGCGCCGTTTTCAGTAACGAACAC‑GTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTCC‑GGATCCAGTTTCATGATGGTCACATTGAGCCCACGGGCTTCAAAAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTCCCCATAGAGGATACGCCCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACgg < 1:89209/251‑1 (MQ=255) gTCTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTCC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGc > 2:193892/1‑250 (MQ=255) cTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGt > 2:64714/1‑251 (MQ=255) cTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCAAGTTTCATGATGGTCACATTGAGGCCCCGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGCAGTTTGGTTGAAAAGACGATGGAATAACTAAGCAGGGAAAGCAGt > 2:628/1‑251 (MQ=255) cAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGGGCAGAGTACCTGGATCGACGTTGAAGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTAGCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGtat > 1:91448/1‑250 (MQ=255) acac‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACAt > 1:297616/1‑251 (MQ=255) ac‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATg > 1:117984/1‑250 (MQ=255) ccGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCtgtg > 1:2728/1‑251 (MQ=255) tGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCcaca > 1:172335/1‑250 (MQ=255) gATTGGGCTAATAGTACCTGGATCGGCGTTGGTGTCC‑GGATCCAGTTTCAGGAGGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTCCCCAGAGAGGATACGCCCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAAc < 2:117984/251‑1 (MQ=255) gggCTCATAGTACCTGGATCGCCGTTGATGCAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCACGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCAAAAACTTTc > 2:563792/1‑251 (MQ=255) ttGATGTCC‑GGATCCAGTTTCATGAGGGTCACATTGAGGCCACGGGCTTCAAGAATGGCGGCGGGGGAGGCTCCGGCAATGCCTTTACCCAGAGAGGATACGCCCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAAGAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAgc < 1:513838/249‑1 (MQ=255) tGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTACTCTTCGCCAgcgc > 2:160836/1‑250 (MQ=255) tAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACCAAAATATAGTTCGTTGTCATGCTGAACCTGAGACGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAACCTTTCATTATCCCTCCTCTTCGCCAGCTCACTAtt > 2:130918/1‑251 (MQ=255) gaC‑GGATCAGGTTTCATGATGGCCACAGGGGCGCCACGGGCTTAAAGAATGGCCGGGAGGGAGGCTGCGGCAATGCCTTTACCCAAAGAGGAGACACCCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAATCTTTCATTATCCCTCCTCTTCGCCAGCGCACTAtt < 2:256069/250‑1 (MQ=255) cgacccgaGTTTCATGAGGGTCACATTGAGGCCACGGGCTTCAAGAATGGCGGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCCCTTCGCCAGCGCACGATTGa < 1:193892/244‑1 (MQ=255) ggATCCAGTTTCAGGAGGGCCACATGGAGGCCACGGGCTACAAGAATGGCTGCGGGGGGGGCTGCGGCAATGCCTTTCCCCAGAGAGAATACGCCCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAGCGCACTATTGaa < 2:297616/251‑1 (MQ=255) aatccattttaaggatgGCCACATTGAGGCCACGGGCTTCAAGAAGGGCGGCGAGGGAGGCTACGCCAATGCCTTTGCCCAGAGAGAATCCGCCCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAGCGCACTATTGaaa < 2:336849/238‑1 (MQ=255) aTCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAg > 1:331166/1‑154 (MQ=255) gTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAGCGCACTATTGAAATCAgg > 1:45923/1‑250 (MQ=255) ttCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAGCGCACTATTGAAATCAGGAgg > 1:23065/1‑250 (MQ=255) gggTCATATAGAGGCGACGGGCGTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCTTTTCCCCAGAGAGGTGACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCGGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCTCCAGCGCACTATTGAAATCAGGAGTGAGaaaa < 2:574005/250‑1 (MQ=255) cacaTTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAGCGCACTATTGAAATCAGGAGTGAGAAAATAGc > 1:259904/1‑251 (MQ=255) tCAAGAATGACTGCGAGGGAGGTTCTGGCAATGCCTTTACCCCGAGAGGATACGCCCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAGCGCACTATTGAAATCAGGAGTGAGAAAATAGCCCCTTTGGGGTAAATGtt < 1:37276/250‑1 (MQ=255) gAATGGCTGCGAGGGAGGCTACGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAGCGCACTATTGAAATCAGGAGTGAGAAAATAGCCCCTTTGGGGTAAATGTTTTTg > 1:47603/1‑250 (MQ=255) gCTGCGAGGGAGGATGCGGCAATGCATTTACCCAGAGAGGATACGAACCAGCCGGTCACACAAATATAGTTCGTTGTCATGCTGAACCTGAGACGTTAGGTTGACAAGACGATGGACTAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAGCGCACTATTGAAATCAGGAGTGAGAAAATAGCCCCTTTGGGGTAAATGTTTTTGACGCa > 2:268857/1‑250 (MQ=255) aagggagggTGCGGCATGGCCTTGACCAAGAGGGGAAACGCCCCCGCCGGTCACAAAAATATAGTACGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTACATTATCCCTCCTCTTCGCCAGCGCACTATTGAAATCAGGAGTGAGAAAATAGCCCCTTTGGGGTAAATGTTTGTGACGCAAATCaa < 2:246195/250‑1 (MQ=255) | CGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAAC‑GCTCGTAGTGCCCCAGGTCCAGGT‑CGGTTTCAGCGCCGTCTTCAGTAACGAACAC‑TTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTAC‑GGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATAACCAAGACGGGAAAGCAGTATACCCGAACATGACCTGTGCCACAAACTTTCATTATCCCTCCTCTTCGCCAGCGCACTATTGAAATCAGGAGTGAGAAAATAGCCCCTTTGGGGTAAATGTTTTTGACGCAAATCAA > NC_000913/2909356‑2909842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |