| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 119,384 | 0 | T | G | 33.9% | 81.7 / 11.5 | 60 | V35G (GTG→GGG) | ampE | protein AmpE |
| Reads supporting (aligned to +/- strand): ref base T (12/27); new base G (20/0); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGG > NC_000913/119134‑119633 | ggctgTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGTGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCTAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑t < 1:232428/247‑1 (MQ=255) ggTTAAGCGGGCACTGCTTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGa > 2:357401/1‑250 (MQ=255) tACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAAc < 2:154388/250‑1 (MQ=255) tgattgCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTTGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGTTGTGAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAATCATTTTTCTCTCGGgc < 2:597340/250‑1 (MQ=255) acccGGATATCGCTAAAAACATGTCGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTAATTACAAGCTTACTGGTGTTAATGTTCGAGCGCCTGTGTTAGTTTGGCTAGCACTGGCAGCTTGATCATCGTGGTTAAGCGTTCTTTCGTCTTG‑TTAAACATTTTCCTCTCGGGCGCACGtt < 2:562319/247‑1 (MQ=255) cGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCat < 1:26061/251‑1 (MQ=255) cTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGGAACAATTTTTTCTCGGGCGCACGTTAGGCATG‑Acccatt > 1:343202/1‑247 (MQ=255) aaaCAGGACGGGCCATTGTGATATTGCGCCGGATCGGAAACCCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCGATTTACAACCTTACTTGTGTTAATTTTCGAGCGCCTGTTGAAGTTTGGCGAGCACTGGCAGCTGGATCATCGTCTGGAAGCGGTCTTTCGGCGGG‑TGAAACATGTTTCTCTCGGGGGCACGTTAGTCATG‑ACCAGTATTGc < 2:534439/251‑1 (MQ=255) aCATGACGCGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGAATCGAGTGCTGGTCCGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGATAATTTTCGAGCGCATGTTTAAGCTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACATTTTTCTCTCGGGCGCCCGTTCGGCATG‑ACCCTTATTGCgg > 1:390479/1‑250 (MQ=255) gACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATgg < 2:212498/250‑1 (MQ=255) ttGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACATTTTTTTCTCGGGCGCCCGTTAGGCATG‑ACCATTCTTGCG‑ATGGGCGTGGCTTTTTTTCTGt > 1:97716/1‑251 (MQ=255) cgcCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTTTCTCGGGCGCAAGTTAGGCATG‑AGCATTATTGCGGATGG‑CGTGGCTTTTTTACTGTTAc > 1:17492/1‑251 (MQ=255) gcCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACATTTTTTTCTCGGGCGCACGGTAGGCATG‑CCCATTATTTCG‑ATGGGGGTGACTTTTTTACTTTTa > 1:18740/1‑249 (MQ=255) gATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCa < 2:505382/251‑1 (MQ=255) aTCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCAt < 1:518340/251‑1 (MQ=255) ggAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACATTTTTCTCTCGGGGGCACGTTAGGCATG‑TACATTTTTGCG‑ATGGGCGGGGCTTTTTTACTGTTACGCGCATTGc > 1:288288/1‑251 (MQ=255) cGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGGACAATTTTTCTCTCGGGCGCGCGTTAGGCGTG‑ACCATTATTTCG‑GTGGGGGGGGCTTTTTTTCTGTTTCGCGCATTGCAGGGAgg > 2:270426/1‑250 (MQ=255) cGGTCCTGCATTTGATTTGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTGTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATGATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGtt < 2:522823/251‑1 (MQ=255) gTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGCTAGGCATG‑ACCATTATTGCG‑GTGGGCGTGGCTTTTTTTCTGTTACGCGCATTGCAGGGGGTATTGtttt > 2:122182/1‑249 (MQ=255) ccTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGAACATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTTTCTCTGGCGCACCTTAGGCATG‑ACCATTATTGCG‑CTGGGCGGGGCTTTTTTTTTGTTTCGCGCCTTTCAGGGAGGATTTTT‑CAAc > 2:115749/1‑251 (MQ=255) ccTGCATTTGATTGGGCACGGTATCGCGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAAGCTTACTGGTGTTAATTTTCGAGCGCCGGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCGTGAAGCGTTCTTTCGGCGGG‑TGAAGCATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCACTATTGCG‑ATGAGCCTGACTTTTGTACTGTTACGCGCATTGCAGGGAGTATTGTT‑Caa < 2:421648/250‑1 (MQ=255) gCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑t < 1:209376/250‑1 (MQ=255) ttGATTGGGCACGGTTTCGTGTGCTGGTCAACAAGGAGGAAACATGACGCTATTTACAACCATACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTAGGGCGAGCACTGGCAGCTTGATCATCGTCATGAAGCGTTCTTTCGGCGGA‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTccc < 1:279280/250‑1 (MQ=255) ggCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGGTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACt < 1:438844/251‑1 (MQ=255) gTTTCGTATGCTGGTCCGCAAGGAGACCACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGGAACACTTTTCTCCCGGGGGCCCGCTAGGCAAG‑ACCACTTTTGCG‑ATTGGCCTGTCTTTTTTTCTGTTACCCGCATTTCAGGGGGTATTGTT‑ccactgtcccaccctcatggtggt > 1:499436/1‑228 (MQ=255) ggTCAGCACGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATAGTCTTGAAGCGTTCTTTCGGCGGGCGGAAAC‑TTTTTTTATCGGGGGCACGTTTGGGCTG‑TACATTATTGCG‑ATGGGGGTGGATTTTTTTCTGTTAAGCGCCATGCAGGGGGTATTTTT‑CAACG‑TTCCCACGCTCCTGGT‑GTgggggctggttgg > 2:372613/1‑240 (MQ=255) ggTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTTCG‑ATGGGCGTGACTTTTTTTCTGTTACGCGCATTTCAGGGAGGATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTgg > 1:337919/1‑251 (MQ=255) gcAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGGTAATTTTCGAGCGCCTGTTTAAGGTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTGTACTGTTACGCGCATTGCAGGGAGTATTGTG‑CAACG‑TGCCCACGCTACTGGT‑GTGGCTGCTGATTGGtt < 2:236198/250‑1 (MQ=255) aaGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAAAATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTTCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGGTGCTGATTGGTTTGCtg > 1:32135/1‑251 (MQ=255) ggAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGGAACATTTTTTTCTCCGGCGCACGTTTGGCATG‑AACCTTTTTTCG‑ATGGGCGTGACTTTTTTTCTTTTTCGCCCATTTCCGGGAGTATTGTT‑CAACG‑GTCCCACGCTACTTGT‑GTGGCTGCTGGTTGGGTTGCtgtg > 1:431843/1‑251 (MQ=255) caacaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACATTTTTTTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGGGTGACTTTTTTTCTGTTACGCGCATTGCAGGGGGGATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTTCTGTGTATTg > 1:221301/1‑251 (MQ=255) acaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGgc < 1:380634/251‑1 (MQ=255) aTGACGCTATTTACAATCTTGCTGGTGTTAATTTTCGAGAGCTTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATATTCGTCTTGAGGCGATCTTTCGGCGGG‑GGAACAATTTTTTTCTCGGGGGCCCGTTAGGCAGG‑ACCAATATTGCG‑GTGGGCGTGACTTTTTTACTGTTACGCGCGCTGCAGGGAGGATTTTTTCAA‑G‑ATTCCACGCTACCGGT‑GGGGGTGGTGTTTGGTTTTCTGTGTTTTggggc > 1:525040/1‑248 (MQ=255) gACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCATGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGGAACATTTTTTTCTTGGGCGCACGTTCGGACTG‑ACCATTATTGCG‑TAGGGCGGGGCTTTTTTTCTGTTTAGCGCACTTCAGGGGGTATTGTT‑CAACA‑TTTCCCCCCTAATGGTTGGGGC‑GGTGATTGGTTTGCTTTGTATTgtaga > 1:376598/1‑245 (MQ=255) cTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAAACTTTTTTTTTCTGGCGCACGTTTGGCCTG‑ACCCTTTTTTCG‑TTGGGCGTGACTTTTTTTTTGTTTCGCCCCTTTCACGGTGTCTTGTT‑CAACA‑TTCCCCCGCTCCTGGT‑GTGGCTGCTGGTTGGTTTTCTTTGTTTTGGCGCAGGtgg > 2:26265/1‑249 (MQ=255) tttACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGCAACATTTTTCTCTCGGGCGCCCGCTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGAATTTTTTATTTTTAAGCGCATTGCCGGGAGTATTTTT‑TAACG‑TTTCCCCGCCCCTGGG‑GGGGCTGTCGATTGGTTTGCCGGGTATTTGCGCAGGGAAAgg > 1:589582/1‑250 (MQ=255) aCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCCCTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑AGAAACATTTTTTTCTCAGGCGCACGTTAGGGACG‑AACCTTCTTGCG‑ATGGGCGGGGCTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAAAG‑TTCCCACGCTCCTGGG‑GTGGCTGCTGGTTGGTTTGGTGTGTATTGGGGCAGGTAAAGTTCggct > 1:581311/1‑248 (MQ=255) aCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGGAACATTTTTTTTTCGGGAGCACGTTAGGCATGGACC‑TTATTGCG‑ATGGGCGTGGCTTTTTTAATGTTATGCGCACTGCTGGGGGTGTTGTT‑CAACG‑TTTCCACGCTCCTGTT‑GTGGGTGCTGATTGGTTTTCTTTGTATTGGCGCAgggacaggttgtct > 2:529101/1‑239 (MQ=255) ccTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCAtt < 2:427218/166‑1 (MQ=255) ccTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAAAATTTTTTTCTCGGGCGCCCGTTAGGCCTG‑ACCATTTTTGCG‑ATGGGCGGGGCTTTTTTTCTGTTTCGCGCAtt > 1:427218/1‑166 (MQ=255) aCTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTTTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTTCTGTTACGCGCATTGCAGGGAGGATTGTT‑TAACG‑TTCCCCCGCTACTGGT‑GTGGCTGCTGTTTGGTTTGTTGTGTGTTGGCGCAGGTAAAGGTCGTCTTTAtt > 1:137033/1‑251 (MQ=255) ggTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTAt < 1:513053/250‑1 (MQ=255) ggTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGGAACATTTTTCTCTCGGGGGCACGTTTGGGCTG‑ACAATTATTTCG‑GTGGGGGTGGCTTTTTTTCTGTTACGCGCCTTTTAGGGAGGATTGTT‑CTACGGTTCCC‑CGCTCATGGT‑GTGGGTGGTGATTTGTTTGCTGTGTATTGGCGCGGGTAAAGGTTGGCTTCATTAtt > 1:77909/1‑250 (MQ=255) tAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACATTTTTCTCTCGGGCGCCCGTTCGGGATG‑AACATTATTGCG‑ATGGGCGTGACTTTTTTTCTGTTACGCGCATTGCAGGGGGTATTTTT‑CAACG‑TTCCCACGCTAATGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGGAAAGTTCGTCTTCATTATCCTGCt > 1:542876/1‑251 (MQ=255) aaTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGGGGATTGTT‑TAACG‑TTCCCACGCTACTGGT‑GTGGGTGGTGGTTGGTTTGCTGTGTTTTGGGGCAGGGAAAGTTCGTCTTCATTATCATggtt > 1:23700/1‑248 (MQ=255) cGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGTCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGa < 1:548972/251‑1 (MQ=255) cGAGCGCCTGGTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGa < 1:55182/251‑1 (MQ=255) aGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACATTTTTCTCTCGGGGGCCCGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTTCTGTTACGCGCCTTGCAGGGAGTATTTTT‑TAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCCGGTAAAGGTCGTCTTCATTTTCATGCTTATCTGAcc > 1:390376/1‑250 (MQ=255) gcgcCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTGCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAg < 1:372613/251‑1 (MQ=255) cgcCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAgc < 1:75302/251‑1 (MQ=255) cTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTTTCTCGGGCGCACGGTAGGCATG‑ACCATTATTGCG‑ATGGGCGGGACTTTTTTACTGTTACGCGCATTTCAGGGAGGATTGTT‑CAAAG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGGGCCGGGAAAGTTCGTCTTCATTATCATGCTTATCTTACAgcttc > 1:463930/1‑249 (MQ=255) gTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACCTTTTTTTTCCGGGGCCACGTTAGGCATG‑ACCCTTATTGCG‑ATGGGCGTGACTTTTTTTATGTTATGCGCCTTGCAGGGGGTATTGTT‑CAACG‑GTTCCAAGCCACTGGG‑GTGGCTGCTGTTTGGGTTGGTGTGTATTGGGGCAGGTAAAGTTCGTCTTCATTATCCTGATTATCTTGCAGCTGCtt > 1:577139/1‑251 (MQ=255) tAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCa < 1:566417/250‑1 (MQ=255) aaGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGTGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACg < 1:262959/251‑1 (MQ=255) aGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAAAATATTTCTCTCGGGCGCCAGTTTGGCATC‑ACCATTATTGGG‑GTGGGGGGGACTTTTTTTCTTTTTAGCCCATTGCCGGGAGGACTTTT‑CAACG‑TTTCACCGCTACTGGT‑GTGGCTGCTGGTTGTTTTTCTGTGTTTTTGGGCCGGTAAAGGTTGTCTTCTTTTTCATTCTTTTTTTACAGCTGCtttccgt > 2:195461/1‑246 (MQ=255) gggCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACATTTTTCTCTCGGGCGCCCGTTAGGCATG‑CCCATTATTTCA‑GTGGGCGGGAATTTTTTTCTGTTTCGCGCCTTGCCGGGAGTATTGTT‑CCACG‑TTCCCACCCTACTGGG‑GTGGGTGCTGATTGGTTTGCTGTGTATTGGGGCAGGGAAAGGTCGTCTTCCTTATCCTGCTTATCTGACAGCTGCTTCACGTAAtt > 1:34583/1‑250 (MQ=255) aGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑GGAAACCTTTTTTTTTCGGGCGCACGCTTGGCATG‑AACATTTTTGCG‑ATGGGCGTGACTTTTTTACTGTTACCCGCATTGCAGGGAGTATTGTT‑CTACC‑TTCCCACGCTAATGGT‑GTGGCTGCTGTTTTGTTTGCTGTGTATTGGCGCCGGTTAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCCTGCCCGt > 1:111609/1‑251 (MQ=255) catcGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGAt < 2:175568/251‑1 (MQ=255) gAAGCGGTCCTTCGGCGGG‑TGAACCATTTGTCTCTCGGGAGCACGTTAGGCATG‑ACAATTATCGCG‑ATGGGCGTGACTTTTGTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCGGATCTGACAGCGGCTTCACGTAATGATAGCCATGCCCGTGCCACGAtggctggcc < 2:132998/250‑2 (MQ=255) aGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCGGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAAc < 2:581311/251‑1 (MQ=255) gg‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTTACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGTCTGGCGAACTCACCATGATTCACg < 1:522297/251‑1 (MQ=255) tGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACgg < 2:223918/250‑1 (MQ=255) tGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACgg > 1:223918/1‑250 (MQ=255) | GGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGG‑TGAAACATTTTTCTCTCGGGCGCACGTTAGGCATG‑ACCATTATTGCG‑ATGGGCGTGACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTT‑CAACG‑TTCCCACGCTACTGGT‑GTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGG > NC_000913/119134‑119633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |