breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
JC JC 306,101 IS2 (–) +5 bp coding (713‑717/1644 nt) ecpD ← fimbrial adhesin EcpD
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
MC JC 339,199 Δ36,219 bp [yahG]mhpE 31 genes
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,632,837 A→G intergenic (‑552/‑53) ydfJ ← / → ynfT putative transporter YdfJ/protein YnfT
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,695,565 C→T G169G (GGG→GGA uidA ← beta‑glucuronidase
RA 1,699,275 C→T intergenic (‑95/‑80) malI ← / → malX DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 4,285,202 A→C T4T (ACT→ACG yjcH ← DUF485 domain‑containing inner membrane protein YjcH
RA 4,285,205 G→A G3G (GGC→GGT yjcH ← DUF485 domain‑containing inner membrane protein YjcH
RA 4,285,320 +A intergenic (‑107/+93) yjcH ← / ← acs DUF485 domain‑containing inner membrane protein YjcH/acetyl‑CoA synthetase (AMP‑forming)
RA 4,285,400 G→C intergenic (‑187/+13) yjcH ← / ← acs DUF485 domain‑containing inner membrane protein YjcH/acetyl‑CoA synthetase (AMP‑forming)
RA 4,285,782 T→C A530A (GCA→GCG acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,285,788 C→T G528G (GGG→GGA acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,285,818 G→A D518D (GAC→GAT acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,285,977 T→C V465V (GTA→GTG acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,285,986 A→G G462G (GGT→GGC acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,285,995 G→A A459A (GCC→GCT acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,285,998 C→G G458G (GGG→GGC acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,001 C→T E457E (GAG→GAA acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,100 G→C T424T (ACC→ACG acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,118 G→A T418T (ACC→ACT acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,202 A→G I390I (ATT→ATC acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,241 A→T R377R (CGT→CGA acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,256 G→A I372I (ATC→ATT acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,268 G→C G368G (GGC→GGG acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,289 G→A I361I (ATC→ATT acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,301 T→C A357A (GCA→GCG acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,286,370 G→C P334P (CCC→CCG acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,287,586 G→A intergenic (‑215/‑25) acs ← / → ytcB acetyl‑CoA synthetase (AMP‑forming)/protein YtcB
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,915 C→T A71T (GCG→ACG)  yjcO ← Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 401670 402396 727 48 [0] [2] 3 [phoA] [phoA]
* * ÷ NC_000913 1694762 1695469 708 3 [0] [0] 6 uidA beta‑glucuronidase
* * ÷ NC_000913 3423908–3424234 3424420–3424238 5–513 3 [2] [2] 3 rrlD 23S ribosomal RNA
* * ÷ NC_000913 4305538 4318237 12700 8 [2] [2] 3 [yjcS]–[phnK] [yjcS],alsK,alsE,alsC,alsA,alsB,alsR,rpiB,yjdP,phnP,phnO,phnN,phnM,phnL,[phnK]
* * ÷ NC_000913 4318274 4324314 6041 3 [2] [0] 27 [phnK]–[phnD] [phnK],phnJ,phnI,phnH,phnG,phnF,[phnD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4016690 (0.000)48 (1.060) 44/472 0.2 99.0% intergenic (+23/‑78) iraP/phoA anti‑adaptor protein IraP/alkaline phosphatase
?NC_000913 402398 = 1 (0.020)coding (652/1416 nt) phoA alkaline phosphatase
* ? NC_000913 1207790 =12 (0.260)10 (0.230) 10/456 1.7 45.2% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 13 (0.300)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780511 (0.230)29 (0.660) 26/456 0.6 71.4% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 13 (0.300)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 43055332 (0.040)19 (0.420)
+CGCCGCTTCA
15/468 1.2 95.2% coding (1065/1986 nt) yjcS linear primary‑alkylsulfatase
?NC_000913 4324315 = 0 (0.000)coding (38/1017 nt) phnD phosphonate/phosphate ABC transporter periplasmic binding protein
* ? NC_000913 = 43055372 (0.040)6 (0.130)
+CCGCTTCA
6/472 2.4 86.1% coding (1061/1986 nt) yjcS linear primary‑alkylsulfatase
?NC_000913 4324315 = 0 (0.000)coding (38/1017 nt) phnD phosphonate/phosphate ABC transporter periplasmic binding protein