Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,287,586	G→A	intergenic (‑215/‑25)	acs ← / → ytcB	acetyl‑CoA synthetase (AMP‑forming)/protein YtcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,287,586	0	G	A	100.0%	95.3 / NA	28	intergenic (‑215/‑25)	acs/ytcB	acetyl‑CoA synthetase (AMP‑forming)/protein YtcB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/13);  total (15/13)

TGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATT  >  NC_000913/4287347‑4287811                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                   tGGTGTGTTTGTGAAATTAGCTCATGCTTTTGTTCTCCTTGTAGGATGTTACTAATATGTGGCATACGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCTGTTATGCATGATGTTTAAAAAGAGGAAGATACTAACTCACTCTa                                                                                                                                                                                                                          >  2:314542/1‑250 (MQ=255)       gtTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGt                                                                                                                                                                                                                   <  1:202766/251‑1 (MQ=255)                tttGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAAATAACTCTAAAGTGGTATTTTACAt                                                                                                                                                                                                          >  2:394888/1‑251 (MQ=255)                        aTGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTAc                                                                                                                                                                                                  <  1:406324/251‑1 (MQ=255)                          gCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGAATAAGCGCTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACa                                                                                                                                                                                                 >  1:189380/1‑250 (MQ=255)                                      cTTGTAGGATGTTAATAATATGAGGCATACACGCTAAATGTAGCGGTCTTGGCTGTTTTGTTTAGAATTTGGGCGACAGATCACGCAAAAGTAGCATTGTGCAAATAAACGGCGGGGTAATTTTTGAAGGTAAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCAAGCTGTTGAAAAAGAGGAAGAAACTAACTAAATATAACGTGGTATTTTAAATGCACTTACAATTGATTAAAGa                                                                                                                                                                                     >  2:196139/1‑250 (MQ=255)                                                      aataTGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGtgtg                                                                                                                                                                     <  1:236362/250‑1 (MQ=255)                                                                        tAAATGTCGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACACAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACAt                                                                                                                                                    >  2:67827/1‑248 (MQ=255)                                                                        tAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACAt                                                                                                                                                    <  1:67827/248‑1 (MQ=255)                                                                                      ttGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTc                                                                                                                                    >  2:400840/1‑250 (MQ=255)                                                                                        ggCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTAGTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCAt                                                                                                                                  <  1:295694/250‑1 (MQ=255)                                                                                          cAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGAc                                                                                                                               >  1:255415/1‑251 (MQ=255)                                                                                                      gTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATGAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCg                                                                                                                   <  2:448560/251‑1 (MQ=255)                                                                                                                   aGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGAAGATATGCATGCTGTTGAAAAAGAGGACGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGAAAACATTCAACGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGCAAGTGTAGGTGCGGATACTGTCGTg                                                                                                       >  2:335884/1‑250 (MQ=255)                                                                                                                        ccGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAATTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTCCTGTCGTGAAAAAc                                                                                                 <  2:361444/250‑1 (MQ=255)                                                                                                                         cGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACa                                                                                                <  1:349372/251‑1 (MQ=255)                                                                                                                                                        gggTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAAt                                                                 >  1:154648/1‑251 (MQ=255)                                                                                                                                                        gggTAATTTTTGAAGGTCAGCAACAAAAGGT‑ATTAAATTTTT‑TGAGAAAAATGTAGTTATGAATTCTGTTAAAAAAGAGAAAGAAACTAAATAACCAAAAAGTGGTATTTAATAGTCATTTAAAATTGATTAAAGAAAACATGCACAGTGTGGT‑TATTTGTTACACAGAGGGGAGAGAAATGTAGTTACAGTGTAGGTGCGGTTCCTGTCTAGAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAAt                                                                 <  2:142493/251‑1 (MQ=255)                                                                                                                                                          gTAATTTTTAAAGGCCAGTAAAAAAAGTTAAGTAT‑ATCTT‑GAAGGAAAATGCGGTTATGCATGTTGTTGAAAAAGAGAAAGATACTAACTACCTCAAAGGTGTTATTTAACATGCACTTAAAATTGATAAAAGACACCTTTCGCATTG‑GGTGTATTTTTTACACATGGGGTCGAGCAATGCGTTGACAGTGGGGGTGCGGTTGTTGTTGAAAAAAAAAATAAAAACCGCCATTGCAACAATGGCGCAATTc                                                               <  1:267993/251‑1 (MQ=255)                                                                                                                                                                 ttGAAGGACAGCGACAAATGTTGCGAAA‑TTCTT‑TGAGTAACATCAAGTTATGCATGCAGTTGAAAAACAGGAAGATACTACCTACATCTAAAATGGTATTTTAAATGTACTTAAAATTTATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAAGGGCGAGAAATCGCATGACAGTGTAGGTGAGGTTACTGTCGTGAAACACAATAAAAAGCGCCATTGCAACAATGGCGCAAttaaaataa                                                         >  2:271881/1‑243 (MQ=255)                                                                                                                                                                    aaGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATg                                                                                                                                      >  1:195111/1‑170 (MQ=255)                                                                                                                                                                    aaGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGcc                                                     >  2:263118/1‑251 (MQ=255)                                                                                                                                                                              acaaAAGTGGATTA‑‑TTCTTGTGGGGAACATGCAGTGATGCTTGCTGTTAAAAAAGGGGAAGATACTACCTACCACTAAAGGGGTATTTTACAGGCATTAAAAATGGTTTAAAGCCACTTTTCGCATTGTGTT‑TTTTTGTTAAAAATAGGGGCGAGAAAGGCCATGAAGGTTTAGGTGCGGTTACTGTCGTGAAAAAAAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACaa                                           <  1:263118/251‑1 (MQ=255)                                                                                                                                                                                                    tGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacgcacgc                       >  2:237107/1‑251 (MQ=255)                                                                                                                                                                                                     gAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGcc                      >  1:249649/1‑251 (MQ=255)                                                                                                                                                                                                            aTGCAGTTAGGCATGCTGTTGAAAAAGAGGAAAATACAAACAAACTCAAAAGGGTTATTTTACATGCACTAAAAATTGATAAAAGACAACATCCACAGTGTGTT‑TATTTGTTACACATGGGGGCGAGAAATGTCATGACAGTGTGGGTGGGGTTGCTGTCGTAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCtt               <  1:237107/251‑1 (MQ=255)                                                                                                                                                                                                                aGTTATGCATGCTGTTGCAAAAGAGGAAGATACTAACTAACTCTAAAGTGGTATTTTACATGCACTTACACTTGATTAAAGACAACATTCACAGTGTGGT‑TCTTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAACACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGc           >  2:459066/1‑251 (MQ=255)                                                                                                                                                                                                                          ggtGTTGAAAAAGAAGAAAATACAAACTAACTCAAAAGTGGTTTTTTATATGTATTTAAAATGGATAAAAAACAACATTCAAGGTGGGGT‑TATTTGTTCCACTGGGGGGCGAGAAATGTCAGGACAGTGAGGGTGCGGTTACTGTCGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGAtt  <  2:85522/248‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                   TGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAA‑TTCTT‑TGAGGAACATGCAGTTATGCATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGT‑TATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATT  >  NC_000913/4287347‑4287811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑