Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	313,543	G→A	intergenic (‑301/‑1748)	ykgR ← / → insE1	putative membrane protein YkgR/IS3 element protein InsE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	313,543	0	G	A	100.0%	104.4 / NA	28	intergenic (‑301/‑1748)	ykgR/insE1	putative membrane protein YkgR/IS3 element protein InsE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

GTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGGAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTT  >  NC_000913/313307‑313775                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         gTCAACCAATTTTTTGACATGTATAACAAATTTGACTAGATGTATTACATCAACTATCATTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTCTCTCCTGGGGTGATATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTAtt                                                                                                                                                                                                                            >  2:153945/1‑251 (MQ=255)            ttttGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAAc                                                                                                                                                                                                                 >  1:261608/1‑251 (MQ=255)                     gTATCACAAATTTGAATAGATGTATTACATCAACTATATTTTCTTGCACCAACGTCTTTGATATATGTCGCATGACATCAGTTCCTGGAATGAGTCTGATCTCACGACTGCCCCAGTCCGGGCGTTGCTTGGTGCTGCGGAGCATATCGCATCTCATCATACTTTCGTATCTCCTGGGGTGTTATACAAGCTATCGTTGTTGGTTACCTGTGAGCGAGATTGAGTTCTATTAAACCGTCATCTCTGCCgtt                                                                                                                                                                                                        >  2:296425/1‑249 (MQ=255)                               tttGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGACAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTgg                                                                                                                                                                                              >  1:241789/1‑251 (MQ=255)                                  gAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGAt                                                                                                                                                                                            <  2:339608/250‑1 (MQ=255)                                        aTGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTg                                                                                                                                                                                     >  1:117621/1‑251 (MQ=255)                                                                          gTCATTGATATATGACGCCTGAAGTCAGTTCCGGGTCTGAGACTGATCTCAAGGCTGGCCCAGTCAGGGCGTTGTTTGGTGCTGCGGAGCATATCGCATCTCATCATAATGTCGTATCTCATGGGGTGTTATACAAGATATCGTTGTTGGTGAACTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTGTGCGAGAACGTGCCGCAGTgc                                                                                                                                                   >  1:419240/1‑250 (MQ=255)                                                                            cATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGAc                                                                                                                                                  <  2:296573/250‑1 (MQ=255)                                                                                      tGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTa                                                                                                                                        >  1:207888/1‑250 (MQ=255)                                                                                      tGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCGCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTa                                                                                                                                        <  2:207888/250‑1 (MQ=255)                                                                                                                    cTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTACCTGAAAGAATTTGTGAGGCTGTATCGGtt                                                                                                          >  1:98476/1‑250 (MQ=255)                                                                                                                           cAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATGGAGTTCTATTAAACCGTCAACTATGCCGTATACATACTGGAGTACGCTGCAGGCACGCCTTATGATAGAGCGTGCGGCAGTGACGTGGTAATTATCGTAAATAATTTGTTAGGCTGTATCTGTTACTCAtt                                                                                                   <  2:204059/250‑1 (MQ=255)                                                                                                                                  ggCCCAGGCCGGGCGTTGATTGGGGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACGATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTGGTGAGGCTGTATCGGTTACGCAttgatttg                                                                                             <  2:51113/249‑1 (MQ=255)                                                                                                                                     ccAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGCTAGt                                                                                        >  1:347645/1‑251 (MQ=255)                                                                                                                                        gTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGtttt                                                                                     <  1:18121/251‑1 (MQ=255)                                                                                                                                          ccGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGACGTAGCGCCTGGGGTGGTATACAAGATATCGTTGTAGGTGACCTGGGAGAGAAAGTGAGTGCTATTAAACCGTCAACTATGCCGGAGACATACTGGATGACACTGCAGGCACGCCTGATGAGAGAACGGGCCTCAGTGACTGGTTAATGAGCGGAAAGAATTTGTGAGGCGGGATCGGTTACTCATTGGTTTGAGAGTTTTa                                                                                    <  2:195221/250‑1 (MQ=255)                                                                                                                                             ggCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCCTATCTCCTGGGGTGTTATACAAACTATCGTTGTTGGTGACCTGGGCGAGAAATTGAGTTATATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAATGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTActct                                                                                >  1:140977/1‑251 (MQ=255)                                                                                                                                                           cTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGaataat                                                                    >  1:225027/1‑249 (MQ=255)                                                                                                                                                                   cATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTa                                                           >  1:138346/1‑250 (MQ=255)                                                                                                                                                                      atCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTAGGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGGATGGGGTACTCATTGATTTGATAGTGTTACTCTCGGGAGAATAATAGATATTTAATc                                                        <  1:308883/250‑1 (MQ=255)                                                                                                                                                                        cGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTACCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCAt                                                     <  2:98476/251‑1 (MQ=255)                                                                                                                                                                                   agaaTGTCGTATTTCCTGGGGTGTTATACAAGATATCGGTGGTGGTGTTCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACCCAGTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGTAAAcc                                          <  2:248709/249‑1 (MQ=255)                                                                                                                                                                                                 ccTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCt                               <  2:138346/248‑1 (MQ=255)                                                                                                                                                                                                 ccTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTc                            >  1:263155/1‑251 (MQ=255)                                                                                                                                                                                                    ggggTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTAATAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCg                           <  1:283532/249‑1 (MQ=255)                                                                                                                                                                                                       gtgtTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGcc                      <  1:10704/251‑1 (MQ=255)                                                                                                                                                                                                                           gttgGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTAGGCCGGATACATACTGGATTACACTGCAGGCACGCCGTATGAGAGAACGTGCCGCAGTTACGGGTTAATTATCTGAAAGAATTTTTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGATAATAATAGATATTTAATCCATTAACGGAACCCAGCCAGTTCCTGTCGATGCCTTAATTTGATCCCATAGttt  <  2:423422/251‑1 (MQ=255)                                                                                                                                                                                                                            ttgGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGttt  >  1:247436/1‑250 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                         GTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGGAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTT  >  NC_000913/313307‑313775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑