Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,286,100	G→C	T424T (ACC→ACG)	acs ←	acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,286,100	0	G	C	96.6%	189.4 / ‑5.9	91	T424T (ACC→ACG)	acs	acetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base C (51/35);  total (51/40)
Fisher's exact test for biased strand distribution p-value = 7.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

TCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATAC  >  NC_000913/4285855‑4286350                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           tCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGa                                                                                                                                                                                                                                                        <  1:16633/250‑1 (MQ=255)        gACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGaaa                                                                                                                                                                                                                                                <  1:95067/251‑1 (MQ=255)        gACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGGAGCGCCAGGCAGCGGCGTGATCATGaa                                                                                                                                                                                                                                                 >  1:18577/1‑250 (MQ=255)         aCGCGCGCAGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGACGAACGGCCGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCACCAGGCAGCGGCGTGATCaagcaac                                                                                                                                                                                                                                               >  2:355054/1‑245 (MQ=255)              cgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAAccgccc                                                                                                                                                                                                                                          >  1:14835/1‑250 (MQ=255)               gcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAAccgcc                                                                                                                                                                                                                                           >  2:447619/1‑249 (MQ=255)                    cgccgcTGAAATACATATTTTTGAAGGTGGAGAATTATGTCTGTTCAAAACG‑TTCGGGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCCCCAGTCTGCCTTCGGTGGCGCCTTCCAGCGGGTTACTTTCGTTATCGACCAGCGCCGGTTGCACGCTGAAGAACGGACGTGTTGCCGATCGGTCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCAGTTTc                                                                                                                                                                                                                                     <  1:214004/250‑1 (MQ=255)                           gAAATACATATTTTTGAAGGTGGAGAAGTAGTTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTCCCTTCGTTATCGACCAGCGCCGTTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTg                                                                                                                                                                                                                               <  1:88456/249‑1 (MQ=255)                              aTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACG‑TTCGTGCTCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGACGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCAAGAAACCGCCAGTTTCGGTCTaccacc                                                                                                                                                                                                                          >  2:183341/1‑251 (MQ=255)                                      tttagAAGGTGGAGAAGTAGGTCTGTTCAAAACG‑TTCGGGATCGCCAAACAGCGTACGCGCCTGACCCGGCCGGGAGTCGGTGATCACCAGGCTGCTTTCGGTAGCGCCTGCCAGGGGTTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGGGATCATAAAACCGCCAGTTTCGGTCTGCCACCAGGTATc                                                                                                                                                                                                                   <  2:100815/246‑1 (MQ=255)                                         ggAAGGTGGAGAAGTAGGCCTGTTCAAACCT‑GTCGTGAGCGCCAAACAGCGTACGCGCCTGCCCCGGCCAGGAGTCGGGGATCACCAGGCTGCCTTCGGTAGCGCTTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGGGATCATTAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGac                                                                                                                                                                                                                <  1:373110/249‑1 (MQ=255)                                          gAAGGTGGAGAAGTAGGTCTGT‑CAAAACGGTTCGTGACCGCCAAACGGCGTCCGCCCCTGCCCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTGCGGTAGCGCCTTCCGGCGGGTTCCCTCCGTTACCGACCGGCGCCGGTTGCACGCCGAAGAGCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGacca                                                                                                                                                                                                              <  2:163612/251‑1 (MQ=255)                                                ggAGAAGTAGGTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTCCGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGAc                                                                                                                                                                                                        >  1:192012/1‑251 (MQ=255)                                                     aGTAGGTCTGTTAAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGAGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGGATCGACCACCgggaatttt                                                                                                                                                                                                   >  2:348821/1‑244 (MQ=255)                                                         gttCTGTTCAAACCG‑TTCGGGATCGCCAAACAGCGTTCGCGCCTGCCCCGGCCAGGAGTCGGTGATCCCCAGGCGGCCTTCGTTAGCGCCTTCCGGCGGTTTACCTTCGTTATCTACCAGCGCGGGTTGCACGCCGAAGAAGGGACGTGTTGCCAAACCGGTTTTCAGCTCGGTAGCGCCGGGCAGCGGCGTGATCTTGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGt                                                                                                                                                                                               <  1:327803/249‑1 (MQ=255)                                                         ggTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCCGGGATCGACCCCCGGACATTTCTCGt                                                                                                                                                                                               >  2:81619/1‑251 (MQ=255)                                                          gTCTGTTCAAAACG‑TTCGTGATCTCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGCTCATGAAACCGCCAGGTTCGGTCTGCCACCAGGTATCGACCACCGGCCATTTCTCGtt                                                                                                                                                                                              >  2:119355/1‑251 (MQ=255)                                                              gTTCAAAACG‑TTCGTGTTCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGcc                                                                                                                                                                                           <  1:257088/250‑1 (MQ=255)                                                                 cAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGCCAAGCGCCGGTTGCACGCCGAAGCACGGACGTGTTGCCGCACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGGTTCGGTCTGCCACCAGGAATAGCCCACCGGACATTTCTCGTTGCCGAtt                                                                                                                                                                                       >  2:367406/1‑251 (MQ=255)                                                                 aaaaaCG‑TTCGGGACCGCCAAACAGCGTACGCCCCTGCCCCGGCCAGGAGTCGGGGATCACCCGGCTCCCTTGGGGAGCGCCTTCCCGCGGGTTACCGTCGTTTTCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTTTTGCCGATCCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGGGATAATGAACCCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAtt                                                                                                                                                                                       <  2:14835/250‑1 (MQ=255)                                                                  aaaaCG‑TTCGGGACCGCCAAACAGCGGTCGCGCCTGCCCCGGCCGGGAGCCGGTGATCACCGGGCTGCCTCCGGTGGCGCCTTCGAGCGGGTTCCCTTCGTTATCGACCGGCGCCGGTGGCCCGCCGAAGAAGGGACGGGTGGCGGAACCGGTTTTAGGCTCGGTACCGCTAGGCAGCGGTGTGATCATGAAACCGCCAGTTTCGGTCTGTCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAttt                                                                                                                                                                                      <  1:185210/251‑1 (MQ=255)                                                                   aaaCG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAtttt                                                                                                                                                                                     >  1:209398/1‑251 (MQ=255)                                                                   aaaCG‑TTCGTGACCGCCAAACGGCGTACGCGCCGGCCCCGGCCAGGAGTGGGTGATCCCCGGTCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCCCGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAtttt                                                                                                                                                                                     <  2:144347/251‑1 (MQ=255)                                                                      cG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGACCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCCGGTACCGACCACCGGACATTTCTCGTTGCCGAttttttt                                                                                                                                                                                  >  2:158356/1‑251 (MQ=255)                                                                       cattCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGTGACGGTGATCACCAGTCTGCCCGCGGTAGCGCCTTCCAGCGGGTTACCTTAGTTATCGACCAGCGCCGGTTACACGCCGAAGAACGCGCGTGTTGCCGATCCAGCTTGCCGCTCGGTAGCGCCCGGCAGCGGCGAGATCATGAAACCGGCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTATCGTTGCCGCttttttt                                                                                                                                                                                  >  2:372775/3‑251 (MQ=255)                                                                         ttCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTCCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTcc                                                                                                                                                                                >  2:182955/1‑251 (MQ=255)                                                                           cGTGATCGCCAACAAGCGCACGCCCCTGCCCCGGCAGGGTGTCGGTGATCACCGGGCTGCCTGCGGTAGCGCCTTCCAGCGGGTTACTTTCGTTATCGACCAGCGCCGGTTGCAAGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATAAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAg                                                                                                                                                                              <  1:338567/251‑1 (MQ=255)                                                                            gTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGCACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGt                                                                                                                                                                             >  1:334375/1‑251 (MQ=255)                                                                            gTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGCGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGCTTTTTTTCCAGt                                                                                                                                                                             >  2:347450/1‑251 (MQ=255)                                                                               aTCGCCAAACCGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGCACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGCAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTAcc                                                                                                                                                                          >  2:149880/1‑251 (MQ=255)                                                                               aTCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCCCCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGCCCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGTTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTAcc                                                                                                                                                                          <  1:348821/251‑1 (MQ=255)                                                                                  cccaacacgcgtccccgcCTGCCCCGGACAGGAGGCGGGGATCCCAAGGTTGCCTTCGGTGGCGCTTTCCAGCGGGTTCCTTTCGTTACCGACCAGCTCCGGTTGCCCGCCGAAGAAGGGACGTGTCGCCGACCCGTCTTTCAGCTCGGTAGCGCCAGGCGGCGGGGTGATAAGTAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACt                                                                                                                                                                       <  2:325236/236‑1 (MQ=255)                                                                                     aaaCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTccc                                                                                                                                                                    >  1:418915/1‑251 (MQ=255)                                                                                           cGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGCTTTTTTTCCAGTACCACTCCCCCGCtt                                                                                                                                                              >  2:299675/1‑251 (MQ=255)                                                                                           cGTACGCGACTGACCCGGAAAGGAGTCGGTGATCACAAGGCTGCCTTCGGTAGCGACTTCCAGCGGGTTACCTTCGTTATAGACCAGCGCCGGTTGCACGCCAAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGACAGGCAGAGGCGTGATCATGAAAACGCCAGTTTCGGGCTGCCACAAGGTAGCGACCACCGGACAGTTCTCGTTGACGATTTTTTTACAGTACAACTCACACGCtt                                                                                                                                                              >  2:384415/1‑251 (MQ=255)                                                                                                  cccTGACCCGGCCAGGAGTCGGTGATCCCCAGGCGGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCGGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGtt                                                                                                                                                       <  1:447582/250‑1 (MQ=255)                                                                                                      gacccggctgggctggggggttcaacAGGCTGCCTGCGGTGGCGCTCTCCAGCGGGTGACCGTCGTTAGCGACCCTCCCGGGTTGCGTGCGGAAGACGGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTGGCGCCAGGCAGCGGCGTGATCAGGAAGCCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCTATTTGTTTCCAGTACCACTCCCACGCTTCCGTGTTGAtt                                                                                                                                                   <  2:192012/226‑1 (MQ=255)                                                                                                           ggCCAGGAGTCGGTTATCCCCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTCCCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTc                                                                                                                                              <  1:447619/251‑1 (MQ=255)                                                                                                           ggCCAGGAGTCGGTGATCCCCAGGCTGCCTTCGGTAGCGCCTTCCAGCGTGTTACCTTCGTTATCGCCCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTc                                                                                                                                              <  1:358359/251‑1 (MQ=255)                                                                                                              cAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTGGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGcc                                                                                                                                           <  1:184367/251‑1 (MQ=255)                                                                                                                  aGTCGGTGATCACCGGGCGGCCTTGGGTAGCGCCTTCCAGCGGGTTACCTTCTTTAGCGACCAGCGCCGGTTGCACGCCGAAGACCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACg                                                                                                                                       <  1:147195/251‑1 (MQ=255)                                                                                                                            ccccAGGCTGCCTTCGGTAGCGCCTCCCAGCGGTTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCgag                                                                                                                               <  1:428566/247‑1 (MQ=255)                                                                                                                            caCCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCCCGGAACCgaga                                                                                                                              >  2:209174/2‑250 (MQ=255)                                                                                                                            caCCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCgag                                                                                                                               >  2:428566/2‑249 (MQ=255)                                                                                                                                         tgggtgtccctcccaagggggTTACCTTCGTTATGGACCCGCGCGGGTTGCACGCCGAAGAACGGACGTGTTGCCAAACCGTCTTTCAGCCGGGGAGCTCCAGCCAGCGGGGGGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAGTTTTTTCCAGTACCACTCCCACGCTTCCTGGTTGATTGGCTCGCCCACGGAACCGAGAATTCGCAGcgac                                                                                                                  <  2:392263/231‑1 (MQ=255)                                                                                                                                           ggtagcgccttCCAGCGGGTTACCTTCGTTATCGCCCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCGCGGTAGCGCCAGGCAGCGGCGTGATAAGGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGcgacgatc                                                                                                              <  1:329925/241‑3 (MQ=255)                                                                                                                                           ggtagcgccttCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGCTTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGACCCGAGAATGCGCAGcgacgatc                                                                                                              >  2:280061/11‑249 (MQ=255)                                                                                                                                              ggcgcttgacggggggTTACTTTCGTTACCGACCGGCGCCGGTGCCACGCCGAAGAACGGCCGTGTTGCCGACCCGGTTTTCAGCGCGGTAGCGCCAGGCAGCGGCGTGTTCATGAACCCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATcggt                                                                                                           <  2:339747/238‑1 (MQ=255)                                                                                                                                               gcgccttCCAGCGGGTTTCCTTCGTTATCGACCAGCCCCGGTTGCCCGCGGAAAAACGGCCGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCTCCAGGTAGCGGCGTGATCATGAATCCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTGGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATcggtc                                                                                                          <  1:122499/245‑1 (MQ=255)                                                                                                                                                cgccttCCAGGGGTTTACCTTCGTTATCGACCAGCGCCGGTGGCCCGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCGCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATcggtcg                                                                                                         <  2:339757/246‑1 (MQ=255)                                                                                                                                                      ccAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCCGCGACGATCGGGCGGTGCCt                                                                                                   >  2:419396/1‑251 (MQ=255)                                                                                                                                                          cGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACGGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTcaa                                                                                               <  1:210570/251‑3 (MQ=255)                                                                                                                                                           gggTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTcaat                                                                                              >  1:120806/1‑248 (MQ=255)                                                                                                                                                            ggTTACTTTCGTTACCGCCCAGCGCTGTTGGCACGCCGAAGACCGGACGTGTTGCCGACCCGGCTTCCATCTCGGTAGCACCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGGACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATc                                                                                             <  1:274832/251‑1 (MQ=255)                                                                                                                                                              ttACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGTACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGGGATCATGAACCCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGGACCGAGAATGCGCAGCGACGATCGGTCGGGGCCTTCAATCGc                                                                                           >  2:388795/1‑251 (MQ=255)                                                                                                                                                                cccTTCGGTATCGACCCGCGCGGGTTGCACGCCGAGGAACGGACGTGTTCCCGACCCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGTGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCtt                                                                                         <  2:334375/250‑1 (MQ=255)                                                                                                                                                                  cTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCCGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGCTGCCTTCACTCGCtttt                                                                                       >  1:226392/1‑250 (MQ=255)                                                                                                                                                                         aTCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCtt                                                                                >  1:247540/1‑251 (MQ=255)                                                                                                                                                                           cGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCCATCGCTTTATCCCCTTcc                                                                              >  2:49275/1‑251 (MQ=255)                                                                                                                                                                            gcccAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTCCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCg                                                                             <  1:182955/249‑1 (MQ=255)                                                                                                                                                                            gACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGAATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGTTTGGCTCGCCCACGGAACCGAGAATGAGCAGGCACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCg                                                                             >  1:149729/1‑251 (MQ=255)                                                                                                                                                                            ccgctcttccgtgctcACGCCGAAGAAGGGACGTGGTGCAGAAGCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGGAACCAAGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGTTTTATCCCCTTCCg                                                                             <  2:57961/236‑1 (MQ=255)                                                                                                                                                                               cAGCGCAGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGATGCTTGGCTCGCCCCCGGAACCGCGCATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGccc                                                                          >  2:450786/1‑250 (MQ=255)                                                                                                                                                                                  cgcCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATc                                                                        >  1:438878/1‑250 (MQ=255)                                                                                                                                                                                  cgcCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCAACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCACCTTCCGCCATcc                                                                       >  1:486784/1‑250 (MQ=255)                                                                                                                                                                                   gcCGGTGGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGTTAGCGCCAGGCAGCGGCGTGATCAGGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAg                                                                      <  1:482078/251‑1 (MQ=255)                                                                                                                                                                                    ccGGTTGCACGCCGAAGAACGGCCGTGTTGACGAACCGGCTTTCAGCTCGGTAGCGCCCGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGCTTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATccgc                                                                     >  1:149574/1‑248 (MQ=255)                                                                                                                                                                                          gCACGCCGAAGAACGGACGTGTTGCCGACCCGGTTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGcgcgcg                                                                <  1:295263/250‑1 (MQ=255)                                                                                                                                                                                           cACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCg                                                                             >  1:57961/1‑236 (MQ=255)                                                                                                                                                                                             cGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATAAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATc                                                            <  1:195130/251‑1 (MQ=255)                                                                                                                                                                                             cGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGATAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCGCCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCCCGCTTCCGGGTTGATTGGCTCGCCCACGGAACCTAGAATGCGCAGCGACGATCGATCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCcgcgcgcgaacc                                                            >  2:467661/1‑247 (MQ=255)                                                                                                                                                                                                 gaagaaCGGACGTGTTGACGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCCTCAGCGCGCGAATCGCCg                                                        >  2:440521/1‑251 (MQ=255)                                                                                                                                                                                                  aagaaCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGTACATTTCTCGTTGCCGCTTTTTTTCCAGTACCCCTCCCACGCTTCCGGGTTGATTGTCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGTTGCCTTCATTCGCTTTATCCCCTTCCGCCATCAGCGCTCGTATCGCCGt                                                       >  1:155317/1‑251 (MQ=255)                                                                                                                                                                                                    gaaCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTgg                                                     >  2:174501/1‑251 (MQ=255)                                                                                                                                                                                                      aCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGCAGCGCCAGGCAGCGGCGTGATCCTGAAACCGCCAGTTTCGGTCTGCCACCAGGCATCGACCACCGGACATATCTAGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTAGGTGCCTTACATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTgggg                                                   >  1:473994/1‑250 (MQ=255)                                                                                                                                                                                                        ggACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCATTCAATCGCTTTATCCCCTTCCGCCAACCGCGCGCGAATCGCCGAgggcgc                                                 >  2:332234/1‑248 (MQ=255)                                                                                                                                                                                                        gaaagtgtTGCCAAACCGGCTTTCAGCTCGGTAGCGCCAGCCAGCGGCGTGCTCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCAAGGAACCGCGAATGCGCAGCGACGAGCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCCCCATCCGCGCGCGAATCGACGTggtcgc                                                 >  2:19367/5‑247 (MQ=255)                                                                                                                                                                                                          gcGTGTTGCCGAACCGGCTTTCAGCTCGGTGGCGCCGGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCGCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTGCGCCATCAGCGCGCGAATCGCCGTTGGCGCgg                                               <  1:158356/250‑1 (MQ=255)                                                                                                                                                                                                              gtTGCCGAACCGGCTTTCAGCTCGGTGGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGtat                                            <  1:280061/250‑1 (MQ=255)                                                                                                                                                                                                              gtTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCCCGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGTCGCGGtat                                            >  2:149469/1‑250 (MQ=255)                                                                                                                                                                                                                   cGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAgaga                                       >  2:335907/1‑250 (MQ=255)                                                                                                                                                                                                                   cGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGAGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAgaga                                       >  1:259281/1‑250 (MQ=255)                                                                                                                                                                                                                        cGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTg                                 >  1:7989/1‑251 (MQ=255)                                                                                                                                                                                                                         gattttCAGCGCGGAGGCGCCAGGCGGCGTCGTGACCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGa                                <  2:18577/248‑1 (MQ=255)                                                                                                                                                                                                                                  cTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCCCCAGGTATCCACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCACCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCCGCGCGCGAATCGCCGTGGGCGCGGTATCGCGACTATTGACCTGATGCt                       >  2:198273/1‑251 (MQ=255)                                                                                                                                                                                                                                    cGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAACCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTg                     >  2:7027/1‑251 (MQ=255)                                                                                                                                                                                                                                        aGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTcc                  <  1:424339/250‑1 (MQ=255)                                                                                                                                                                                                                                           ccgagccAGCGGCGGGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTccacca              <  2:209398/245‑1 (MQ=255)                                                                                                                                                                                                                                            ccAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCcaccac             >  1:73726/1‑251 (MQ=255)                                                                                                                                                                                                                                             cAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCCCCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGCTTGGCTCGCCCACGGAACCGCGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCaccacc            >  1:205940/1‑251 (MQ=255)                                                                                                                                                                                                                                              aGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGt                                              >  1:259692/1‑216 (MQ=255)                                                                                                                                                                                                                                                       ggTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATAc  <  1:81619/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                           TCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACG‑TTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATAC  >  NC_000913/4285855‑4286350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑