Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,699,275	C→T	intergenic (‑95/‑80)	malI ← / → malX	DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,699,275	0	C	T	100.0%	106.1 / NA	30	intergenic (‑95/‑80)	malI/malX	DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/13);  total (17/13)

CGTTCTCCTGTGGCGGTAGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGCCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGC  >  NC_000913/1699062‑1699519                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     cgTTCTCCTGTGGCGGTATATATTCGCCTTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACTCGCCCGCAGCCAGCTCAACATCATGAATGGTTATTTTTTTGGCGGTAGCTATGCAGATGACCTTCTCCCTGATTATGTGACAGATAAAACGTTTTTCCTTTTATTTATCTTATTCCCTCTATTATCCTTGCGTAATGTGATGTATGTCTCACTAAAAGTTGATAAAACGTTTTATCTTCTcgcg                                                                                                                                                                                                                 <  2:220600/251‑1 (MQ=255)                               ttGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTAc                                                                                                                                                                                    <  1:390083/250‑1 (MQ=255)                                  ccACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACgagg                                                                                                                                                                                <  1:190501/251‑1 (MQ=255)                                    gagctgCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACgaggag                                                                                                                                                                              <  1:155386/246‑1 (MQ=255)                                         gCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACgaggag                                                                                                                                                                              >  2:155386/1‑246 (MQ=255)                                                cGAAACGGTACTTACCGACACGCCCGAAGCCAGCCCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGAAGATGACCTACTCCCTGCTTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTCTTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGAc                                                                                                                                                                  >  2:483684/1‑251 (MQ=255)                                                 gAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGCATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATACAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATACAACGTTTTATCTTCTCGCGCAATTTACTGAATACAGATTGTTCTCTACGAGGAGTCGTTTTATGACg                                                                                                                                                                 >  2:272997/1‑251 (MQ=255)                                                                        cGCAGCCAGCGCAACAGCATGAAGGGTTATTTGTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTGTTACCTTTTATTTATCTTAGACCCGCTATTATCGTTGCGTAATGTGAGTTATGTCTCACGAAAAGTTGATAAAACGGTGTATCTGCGCGCGCAATTTACGGAAGCCAGATTGGTCACTACGAGGAGTCGTGTTATGACGTCTAAAACAGCGCCGAAGGTCAc                                                                                                                                          <  2:68397/251‑1 (MQ=255)                                                                            gccagcGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGAAAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTCTTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTAAGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCtg                                                                                                                                      >  1:44637/1‑251 (MQ=255)                                                                                                ggTTATTTTTTTGGCGGTTGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCTTTGCGTAATGTGAGTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACTGCGAAAACAGCACCGAAAGTCACGCTGTGGGATTTCTTCCAGCAGtt                                                                                                                  <  2:183932/251‑1 (MQ=255)                                                                                                           tgccGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCCGCCGTTAGGCAAAACCt                                                                                                       >  2:350634/4‑251 (MQ=255)                                                                                                            ggCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCtt                                                                                                      <  1:279463/251‑1 (MQ=255)                                                                                                              cGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCa                                                                                                    <  2:162203/251‑1 (MQ=255)                                                                                                                   gCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGtt                                                                                                >  1:91205/1‑250 (MQ=255)                                                                                                                      aTGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACgg                                                                                                                                                                >  1:49182/1‑183 (MQ=255)                                                                                                                      aTGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACgg                                                                                                                                                                <  2:49182/183‑1 (MQ=255)                                                                                                                          aGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTgg                                                                                        <  1:215511/251‑1 (MQ=255)                                                                                                                                                 tgACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCAtt                                                                 >  2:106874/1‑251 (MQ=255)                                                                                                                                                  gACAGATAAAACTTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTa                                                                <  2:296756/251‑1 (MQ=255)                                                                                                                                                     aGATAAAACGTTTTACCTTTTCTTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAACACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGc                                                             >  2:65283/1‑251 (MQ=255)                                                                                                                                                        tAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATg                                                                                                  <  1:405616/211‑1 (MQ=255)                                                                                                                                                        tAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATg                                                                                                  >  2:405616/1‑211 (MQ=255)                                                                                                                                                        tAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCg                                                          >  1:344502/1‑251 (MQ=255)                                                                                                                                                              gTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTtctc                                                                                                                                                                                       <  1:34841/120‑1 (MQ=255)                                                                                                                                                              gTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTtctc                                                                                                                                                                                       >  2:34841/1‑120 (MQ=255)                                                                                                                                                                  tACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGCTAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGACAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGAGGCATTATGCTCGGCATTGGt                                                  >  1:57880/1‑249 (MQ=255)                                                                                                                                                                           tttatCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGCGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCtt                                         >  1:21137/1‑249 (MQ=255)                                                                                                                                                                                     aCCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAAGTTGATAAAAAGTTTTATCTTCTCGCGCACTTTACTGCATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGTCGGCGAAAACAGCACCGACAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAAGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCatgat                             >  2:83556/1‑251 (MQ=255)                                                                                                                                                                                                   ttGCGTAATGTGATTTATGTCTCACTAAAATTTGATACAACGTTATATCTTCTCGAGCAATTTACTGAATCAAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATCATGCTCGGAATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTgct               >  1:158543/1‑249 (MQ=255)                                                                                                                                                                                                                 ttATGTCTCACTAAACTTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGc  >  1:379246/1‑250 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     CGTTCTCCTGTGGCGGTAGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGCCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCTGGGC  >  NC_000913/1699062‑1699519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑