Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,325,6440CT98.0% 165.5 / NA 50S33N (AGC→AAC) yjdNPF06983 family protein YjdN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (23/26);  minor base G (0/1);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGC  >  NC_000913/4325397‑4325880
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            
ggCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGa                                                                                                                                                                                                                                           <  1:77825/251‑1 (MQ=255)
    aTTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGAtttt                                                                                                                                                                                                                                       >  1:405033/1‑251 (MQ=255)
       tCGATTTTTCCGTTAGCAGCAAGATTGTCAAACCAGCGTTTTCCGTCTTCGACCTGTTGCGAATCGAGCACCATCGTAAAGCCGGAGTAGCTGTCTTTGCCTGACGGCATGGCATCGCGCATCATGATGGCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTTGCATTTCGCCGAAGTTGATTTtat                                                                                                                                                                                                                                     <  2:265886/250‑1 (MQ=255)
                 cGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATGTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTcc                                                                                                                                                                                                                          <  2:304489/251‑1 (MQ=255)
                                tGTCAATCCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAacgtacgt                                                                                                                                                                                                           >  1:136716/1‑251 (MQ=255)
                                              tgtCCTTCTTCGTCCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCAGGATGTCGCTTCCGGCAATGCGCACATTGGCGTTGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGAGTTTATAGAGCAGTTCCGCGCCCAACGTGCGTTGATAATAGGCaa                                                                                                                                                                                              <  2:73501/248‑1 (MQ=255)
                                                 ccTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATcg                                                                                                                                                                                           <  1:158769/250‑1 (MQ=255)
                                                  cttcttCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGACGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATcgcg                                                                                                                                                                                         >  1:291165/1‑251 (MQ=255)
                                                   ttcttcGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATCGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGt                                                                                                                                                                                        >  2:284052/1‑251 (MQ=255)
                                                     cttcGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCg                                                                                                                                                                                      >  1:177623/1‑251 (MQ=255)
                                                       tcGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCgg                                                                                                                                                                                     <  2:374561/250‑1 (MQ=255)
                                                       tcGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCg                                                                                                                                                                                      <  1:294647/249‑1 (MQ=255)
                                                          cccTGTTGCGAATCGAGCCCCAGGGTAAAGCCGGAGTATCTGGCTTGTCCTGACGTCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCTTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTTGCATTTCGCCGAAGTTGATTTTATAGAGCATTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACa                                                                                                                                                                                 <  2:282442/250‑1 (MQ=255)
                                                                     agcGAGCACCAGCGTAAAGCCGGAGTAGCTGGCGTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGTCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTGCTCGGCGCTGACCTGCGCTGAGTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCaa                                                                                                                                                                      <  2:124399/249‑1 (MQ=255)
                                                                         aGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAaga                                                                                                                                                                  <  1:249515/251‑1 (MQ=255)
                                                                                      aGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAGGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATACTAGGCAATCGCGTCGGAACAGTCACCGGCAAAAGAGAGGTAGGGACtt                                                                                                                                                     >  1:94331/1‑251 (MQ=255)
                                                                                              tAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAACAGAGAGGTAGGGACTTAACGGCat                                                                                                                                             >  2:387303/1‑251 (MQ=255)
                                                                                                 cTGGCTTTTCCTGACGGCATGGCCTCGCTCATCATGATGTCGCTTCCGGCCATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGCAATTACATTCCGGACGGGCAGTTCTCGGCGCTGTCCTGCGCTGCTTTTGGCATTTCGCCGCAGTTGATTTTATAGAGCAGTTCCGCGCCCCACGTACGTTGATCATAGGCACTCGCGTCGGAACAGTTACCGGCAAAAGAGAGGGAGGGACTTCAAGGCatggt                                                                                                                                          >  2:432870/1‑249 (MQ=255)
                                                                                                  gggCTTTTCCTTACGGCCGGGCATCGCTCATCCAGATGTCGCTTCCGGCAATGCGCGCGTTGGTGTGGGCGAGGGGGGTATCAGGAAATTGCATGCCGGAAGGGCAGTTCTCGGCGCGGTCCTGCGCGGATTTTGGCATTTCGCCGAAGTGGATTTGATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAAACGCGTCGGGACGGTTACCGGCAAAAGAGAGGTAGGTACTTAACGGCatgatg                                                                                                                                         <  2:404049/250‑1 (MQ=255)
                                                                                                   tgCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCatgatg                                                                                                                                         <  2:393789/249‑1 (MQ=255)
                                                                                                                ggcatggcatCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTaa                                                                                                                           <  1:219666/251‑1 (MQ=255)
                                                                                                                  catggcatCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAaga                                                                                                                         <  1:425025/251‑1 (MQ=255)
                                                                                                                   atggcatCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTACCGGCATGATGGTGACCTCAGTTAag                                                                                                                          >  2:226745/1‑249 (MQ=255)
                                                                                                                      gcatCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAg                                                                                                                     <  2:177623/251‑1 (MQ=255)
                                                                                                                       catCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCTGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTGCCGCGCCCAACGTACGTTGATAATTGGCAATCGCTTCGGAACAGTTTCCGGCAAAAGAGATGTATGTACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGc                                                                                                                    <  1:266691/251‑1 (MQ=255)
                                                                                                                            cTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGtt                                                                                                               >  1:117425/1‑251 (MQ=255)
                                                                                                                             tcatcaTGATGTCGCTTCCGGCAATGCGCACATTGGCGAGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGGACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGtt                                                                                                               >  1:62209/1‑250 (MQ=255)
                                                                                                                              catcatGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTaa                                                                                                             <  2:145546/251‑1 (MQ=255)
                                                                                                                               atcatGATGTCGCTTACGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTa                                                                                                              >  1:99074/1‑249 (MQ=255)
                                                                                                                                 catGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAgtg                                                                                                          >  1:97156/1‑251 (MQ=255)
                                                                                                                                     atgTCGTTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGt                                                                                                      >  1:138135/1‑251 (MQ=255)
                                                                                                                                         cGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAAGCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCg                                                                                                   <  1:79300/250‑1 (MQ=255)
                                                                                                                                          gCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTGTATAGAGCAGTTCCGCGCCCAACGTACGTGGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGaa                                                                                                 <  2:470051/251‑1 (MQ=255)
                                                                                                                                                 gCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTtat                                                                                           >  1:343915/1‑250 (MQ=255)
                                                                                                                                                    aTGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGGTAAGTGTAGTTCGAAATTTATAc                                                                                         >  2:451861/1‑249 (MQ=255)
                                                                                                                                                                                      gAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCg                                                     <  1:387303/251‑1 (MQ=255)
                                                                                                                                                                                        aaTTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGc                                                    >  2:428068/1‑250 (MQ=255)
                                                                                                                                                                                              aTTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGc                                             >  1:318474/1‑251 (MQ=255)
                                                                                                                                                                                                tCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGc                                             >  1:485478/1‑249 (MQ=255)
                                                                                                                                                                                                 ccGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGt                                          <  2:405033/251‑1 (MQ=255)
                                                                                                                                                                                                 ccGGAAGAGCAGTTCTCGGCGCTGTCCTCCGCTGCTTTTGGCATTTCGCCGCAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGCACAGTTACCGGCAAAAGAGAGGTAGGGCCTTAACGGCATGATGGTGACCTCAGTTACGAGAAGCCAGTTAAGTGTAGTTCGAACTTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGcctccagcagt                                          >  2:23592/1‑241 (MQ=255)
                                                                                                                                                                                                    gAAGGGCAGTTCTCGGGGCTGTCCTGCTCTGAGTTTGCCATTTCGCCGAAGGTGATATTGTAGAGCAGTGCCGCACCCAACGTCCGTTGATAGGAGGCAATCGCGTCGGAACAGTTACCGGCAAGAGAGAGGTAGGGACGTAACGGCATGATGGTGTCCTCAGTTAAGAGAAGCCAGTTAAGTGTAGGTCGAAATTTATACAGATGAGATGCGGCCTGATAAGACGCTCGAGCGTCGCATCAGGCAGTCgg                                       <  2:445489/251‑1 (MQ=255)
                                                                                                                                                                                                                  ggCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGCGCAGCTCCCCGCCCAAAGTACATTGATAATAGGCAATCGCACCGGCACAGTTACCGACACAAGAGAGGTAGGAACTTAACGGCATGATGGTGACCTCAGTTAAGCGAAGCCCGTTAAGTGTAGTTCGAAATTTATACAGCTGAGAGGCGGCCAGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGAt                         >  1:206537/1‑251 (MQ=255)
                                                                                                                                                                                                                       tGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGAGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATgcggc                    >  1:163967/1‑251 (MQ=255)
                                                                                                                                                                                                                        ggccTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATgcggc                    <  1:451861/248‑1 (MQ=255)
                                                                                                                                                                                                                                  gATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTAGCCGGATGCGGCGTAAACGCCt          <  1:375183/250‑1 (MQ=255)
                                                                                                                                                                                                                                       tGGCATTTCGCCGACGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTa                                                                                                        >  1:321903/1‑151 (MQ=255)
                                                                                                                                                                                                                                       tGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTa                                                                                                        <  2:321903/151‑1 (MQ=255)
                                                                                                                                                                                                                                       tGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATcc     <  1:233852/250‑1 (MQ=255)
                                                                                                                                                                                                                                         gCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc  <  2:485478/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            
GGCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGC  >  NC_000913/4325397‑4325880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑