| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,285,788 | C→T | G528G (GGG→GGA) | acs ← | acetyl‑CoA synthetase (AMP‑forming) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,285,788 | 0 | C | T | 98.7% | 262.2 / NA | 77 | G528G (GGG→GGA) | acs | acetyl‑CoA synthetase (AMP‑forming) |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (40/36); minor base G (0/1); total (40/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGC > NC_000913/4285543‑4286031 | aTTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAg < 1:213183/251‑1 (MQ=255) ttGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATCCCTCCTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGAc < 2:148106/250‑1 (MQ=255) ccGGAGCGGGTGTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGGCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCGGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTg < 1:52700/251‑1 (MQ=255) ggAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGAc > 1:392263/1‑251 (MQ=255) ggTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCACTCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACCGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCACACTTCGGCTGCGCCACCAGCGCCGACTCCATCTCCGCCGTTCCCAGACGGTGACCgg > 2:447582/1‑248 (MQ=255) ggTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGgaga < 1:454785/251‑1 (MQ=255) ggCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTc > 1:100815/1‑249 (MQ=255) agtgaggcGGTCCAGTGCAGCCCGGCTGGCGCCGCCAGCGGGCCAATTTCTTTGCGCCCCCAGTTGCTGATGTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTATGCGTGACGTAGGCGTAGATCGTCTGACCTTTAATATTGTGCGGAATACCTACTTCGGCGGCTTCTTCAATCTGCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGGTCCCAGACGGTTACCGGAGACGTTCAGCAc < 2:303914/244‑1 (MQ=255) aGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCAc > 1:293793/1‑251 (MQ=255) tCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACatcgtc > 1:323378/1‑251 (MQ=255) ggTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCCGACGGTGACCGGAGACGTTCAGCACATCGTccc > 2:88456/1‑250 (MQ=255) gcagcaCGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCg < 1:216796/251‑1 (MQ=255) ccGTCTGGCGGCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATCCCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACGCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTAt < 2:239084/250‑1 (MQ=255) tGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGACGACTCCATCTCCGCCGTTCCCCGCCGGTGACCGGAGACGTTCAGCCCCTCGTCCACCCGCCCGGGTATCCAGt > 2:243120/1‑251 (MQ=255) gTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAAt > 1:340264/1‑250 (MQ=255) gCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGcc > 2:149399/1‑251 (MQ=255) ccAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGccc > 2:329925/1‑250 (MQ=255) cGGGCCAATCTCTTTACGCACCCCGTTGCGGACTTCTGCGTAAAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACAGGAGACGTTCAGCCCATCGTCCACACGCCCGGATATCCAGTAATAGCCATCtt > 2:449623/1‑251 (MQ=255) gggCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTc < 1:21916/251‑1 (MQ=255) ccAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATc < 1:228357/251‑1 (MQ=255) cACCCAGTTGCGGCATTGTGCGAACAGTTCTGGTGACGGTTCCTCAGCGTGAGTAAGCGTGCCGTAGGCCTAGCTCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGAGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTACATCTCCGCCGTTCCCAGACGGTGACCGGCGACGTTCAGCACATCGTCCACACCCCCGGTTATCCAGTAATAGCCATCTTTATCCCGCCGCGCGCCGt > 2:223472/1‑251 (MQ=255) cccAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTcg > 1:416429/1‑251 (MQ=255) ccAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCAGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCCTCGGCACTCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGCCCGGAGCCGTTCAGCACATCGGCCACACGCCCGGTTATCCAGTAATGACCATCTTCATCGCGCCGCGCGCCGTcgc > 1:433188/1‑251 (MQ=255) gTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGtt < 1:286439/208‑1 (MQ=255) gTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGtt > 2:286439/1‑208 (MQ=255) gTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGt < 2:132113/207‑1 (MQ=255) gTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGt > 1:132113/1‑207 (MQ=255) ggCGGACTTGTGCGTACAGTGGTGGTGACGGTTCCGCCCCGTGATTAAGCGGGACGGAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCAGCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCAGCTTCATCTCGACGCGCGCCGTCGCCGCTg < 2:25264/250‑1 (MQ=255) tCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATGAAGTGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGTGGCGTCGGCAATCGTCGGATGCGCCACCAGCGCCGACTCAATCGCCGCCGTGCCCAGACGGTGACCGGAGACGGTCAGCACATCGTCCACACGCCCGGTTATCCAGTAAGAGCCATCGTCATCGCGACGCGCGCCGTCGCCGCTGAAATACa < 1:462483/250‑1 (MQ=255) gcTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATGGTGCGGAATTCCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACa < 2:340264/249‑1 (MQ=255) gCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTTGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGATACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACatat < 1:235149/250‑1 (MQ=255) aCAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATAttttt < 1:256339/250‑1 (MQ=255) aGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGa > 1:241951/1‑250 (MQ=255) gTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAg < 1:52038/251‑1 (MQ=255) ttCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAg < 1:98674/250‑1 (MQ=255) cTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTg > 1:408566/1‑251 (MQ=255) tGGTGCCGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGAGCGCCTGACCTTTAATATTGTGCGGAATCCCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTGGAAGGTg < 2:484441/250‑1 (MQ=255) gTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGgag < 2:241951/251‑1 (MQ=255) tGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCCCCCGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCCCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTggcg > 2:184367/1‑248 (MQ=255) gACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGTCAATCTTCGGATGCGCCACCAGCGCCGACGCAATCTCCGCCGTTCCCAGACGATGACCGTAGACGTTCAGCTTATCGGCCACACGCCCGGTTATCCAGTAATAGCCAGCTTCATCGGGACGCGCGCCGTCGCGGCTGAAATACATATTTTTGAAGGTGGAGaa < 1:444964/251‑1 (MQ=255) gACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGGTCAGCACATCGTCCACACGCCCGGTTATCCAGGAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGAGgaga > 2:482078/1‑250 (MQ=255) gggTTCCTCCCCGTGATAAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGt < 2:96625/250‑1 (MQ=255) cGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGAGCGCCTGACCTTTAATAGTGTGCGGAATACCTACTACGGCGGCTGCGGCAATCTTCGGATGCGCCAACAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCAGCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGt < 2:185871/251‑1 (MQ=255) ggTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGt > 1:40825/1‑250 (MQ=255) cctccCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGCGAAGGAGGTc > 2:461889/1‑251 (MQ=255) ccccGTGATTAAGCGTGTCGTAGGCGTAGACCGCCTGTCTCTGAATATTGTGCGGAATCCCTACTAGGGCGGCTTCGGCAATCTCCGGATGCGCCCCCAGCGCCGACTCAAACTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCAGCGCGACGCGCGACGTCGCGGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCt < 2:433188/249‑1 (MQ=255) ccGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTCGGTCTGTTc > 2:358359/1‑251 (MQ=255) ttAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACg > 1:406602/1‑251 (MQ=255) aaGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGt < 1:48462/250‑1 (MQ=255) gcgtgagttAGGCGTAAATCCCCTGACCTTTAATATTGTGCGCAATCCCTCCTACGGCGGCTTCGGCAATCTTCGGATGCCCCCCCAGCCCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCg < 2:92193/243‑1 (MQ=255) aCGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATc > 2:147195/1‑251 (MQ=255) gATCGCCTGACCTTTAATATTGTGCGGAATACCTACTCCGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACatat > 1:71335/1‑197 (MQ=255) gATCGCCTGACCTTTAATATGGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGTATGCGCCCCCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACTCGCGCCGTCGCCGCTGAAATACatat < 2:71335/197‑1 (MQ=255) cTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTg > 1:25121/1‑250 (MQ=255) cGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGt < 1:315995/251‑1 (MQ=255) cGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCCTCGCCGCTGAAATACCTATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGt > 2:210570/1‑251 (MQ=255) ggAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGCAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAggc > 2:200954/1‑249 (MQ=255) ggAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGAGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCCGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTc > 2:295263/1‑251 (MQ=255) aCCTACTACGGCGGCTTCGGCAATCTTCGGATGGGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCCGCACAACGTCCACAAGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGGGGAGAAGTAGGTCTGTTAAAAAAGTTCGTGGTCGCCAAACAGCGTACGCGCCTGACCCCGCCAGGAGGCgggg > 2:195130/1‑248 (MQ=255) taatacatcggcATCGGCAAACTTCGGATTCCCCCCCAGCGCCGACTCAATATCCGCCGATCCCAGACGGTGACCGGAGACATTCAGCACATCGTCCACACGCCCGGGTATCCAGTAATAGGCATCTTCATCGCGACGCGCGCCCTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTATGTCTGTTCCACACGTTCGTGATACCCAAACAGCGTACGCGCCTGACCCGGcccggcagcggtgatc > 2:122499/9‑236 (MQ=255) actacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGAtca < 1:149399/250‑3 (MQ=255) ctacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCAcc > 2:83563/1‑251 (MQ=255) ctacGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCAcc > 1:298778/1‑251 (MQ=255) ccggcggCTCCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGGTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCa < 2:147114/249‑1 (MQ=255) ggcggcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGc > 1:339747/1‑251 (MQ=255) gcTTCGGTAATCTTCGGATGCGCCACCAGCGCAGACCCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACTTTCAGCACATCGTCCTCACGCCCGGTTATCCAGTAATAGCCAGCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATGTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTCCGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCtgtc < 2:38399/251‑4 (MQ=255) aaTCTTCGGATGCGCCACCAGCGCCGACTCACTCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTCTCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGt > 1:269188/1‑249 (MQ=255) aaTCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGACCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGCAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGt > 2:271329/1‑249 (MQ=255) tCTTCGGATGCGCCACCAGCGCCGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGtagcg > 1:127876/1‑247 (MQ=255) aGCGCAGACGCAATCGCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACTCGCCCGGTTATCCAGTAATAGCCATCGTCATCGCGACGCGCGCCGGCGCCGCTGAAATACATAGGTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTCCGCGCCTGACCCGGCCAGGAGTCGTTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTAcc < 2:416429/251‑1 (MQ=255) ccGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGCAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCt > 1:224525/1‑221 (MQ=255) ccGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTGGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCTTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCt < 2:224525/221‑1 (MQ=255) ccGACTCAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCAGCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCg < 2:298778/251‑1 (MQ=255) cAATCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATc < 1:354121/250‑1 (MQ=255) aaTCTCCGCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCg > 1:270316/1‑250 (MQ=255) ccccgCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCa < 2:427509/247‑1 (MQ=255) ccgCCGTTCCCAGACGGTGACCGGAGACGTTCAGCACATCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGGCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATCACCAGGCTGCCTTCGGTAGCGCCTTCCAGCGGGTTACCTTCGTTATCGACCAgc < 1:461889/249‑1 (MQ=255) | ATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTCTGGTGACGGTTCCTCCCCGTGATTAAGCGTGACGTAGGCGTAGATCGCCTGACCTTTAATATTGTGCGGAATACCTACTACGGCGGCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGC > NC_000913/4285543‑4286031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |