| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,286,289 | G→A | I361I (ATC→ATT) | acs ← | acetyl‑CoA synthetase (AMP‑forming) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,286,289 | 0 | G | A | 98.7% | 248.9 / NA | 80 | I361I (ATC→ATT) | acs | acetyl‑CoA synthetase (AMP‑forming) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (40/38); minor base C (1/0); total (42/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
CGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAAC > NC_000913/4286042‑4286533 | cGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATAAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATc < 1:195130/251‑1 (MQ=255) gaagaaCGGACGTGTTGACGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCCTCAGCGCGCGAATCGCCg > 2:440521/1‑251 (MQ=255) aagaaCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGTACATTTCTCGTTGCCGCTTTTTTTCCAGTACCCCTCCCACGCTTCCGGGTTGATTGTCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGTTGCCTTCATTCGCTTTATCCCCTTCCGCCATCAGCGCTCGTATCGCCGt > 1:155317/1‑251 (MQ=255) gaaCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTgg > 2:174501/1‑251 (MQ=255) aCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGCAGCGCCAGGCAGCGGCGTGATCCTGAAACCGCCAGTTTCGGTCTGCCACCAGGCATCGACCACCGGACATATCTAGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTAGGTGCCTTACATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTgggg > 1:473994/1‑250 (MQ=255) ggACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCATTCAATCGCTTTATCCCCTTCCGCCAACCGCGCGCGAATCGCCGAgggcgc > 2:332234/1‑248 (MQ=255) gaaagtgtTGCCAAACCGGCTTTCAGCTCGGTAGCGCCAGCCAGCGGCGTGCTCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCAAGGAACCGCGAATGCGCAGCGACGAGCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCCCCATCCGCGCGCGAATCGACGTggtcgc > 2:19367/5‑247 (MQ=255) gcGTGTTGCCGAACCGGCTTTCAGCTCGGTGGCGCCGGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCGCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTGCGCCATCAGCGCGCGAATCGCCGTTGGCGCgg < 1:158356/250‑1 (MQ=255) gtTGCCGAACCGGCTTTCAGCTCGGTGGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGtat < 1:280061/250‑1 (MQ=255) gtTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCCCGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGTCGCGGtat > 2:149469/1‑250 (MQ=255) cGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAgaga > 2:335907/1‑250 (MQ=255) cGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGAGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAgaga > 1:259281/1‑250 (MQ=255) cGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTg > 1:7989/1‑251 (MQ=255) gattttCAGCGCGGAGGCGCCAGGCGGCGTCGTGACCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGa < 2:18577/248‑1 (MQ=255) cTCGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCCCCAGGTATCCACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCACCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCCGCGCGCGAATCGCCGTGGGCGCGGTATCGCGACTATTGACCTGATGCt > 2:198273/1‑251 (MQ=255) cGGTAGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAACCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTg > 2:7027/1‑251 (MQ=255) aGCGCCAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTcc < 1:424339/250‑1 (MQ=255) ccgagccAGCGGCGGGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTccacca < 2:209398/245‑1 (MQ=255) ccAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCcaccac > 1:73726/1‑251 (MQ=255) cAGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCCCCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGCTTGGCTCGCCCACGGAACCGCGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCaccacc > 1:205940/1‑251 (MQ=255) aGGCAGCGGCGTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGt > 1:259692/1‑216 (MQ=255) gcgTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCAt > 1:356072/3‑250 (MQ=255) ggTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATAc < 1:81619/251‑1 (MQ=255) cgTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGCGAATATTGACCTGATGCTTGTCCACCACCTGCGCCCTAc > 2:356091/2‑251 (MQ=255) gTGATCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACg > 1:474593/1‑251 (MQ=255) aTCATGAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGc > 1:365928/1‑251 (MQ=255) gAAACCGCCAGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAata > 1:437260/1‑208 (MQ=255) cgccaGTTTCGGTCTGCCACCAGGAATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGc > 1:182825/1‑251 (MQ=255) tCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGttcg < 1:347450/250‑3 (MQ=255) ggTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTa < 1:174501/251‑1 (MQ=255) gCCACCAGGAAACGACCAACGGCCATTTCTCGTTGACGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGATGATTGGCTCGCCCACGGAAAAGAGACTGCGCAGCGACGATAGGGCGGTGCCTTCAATCGCTTTATACCCTTCCGCCATCAGCGCGCGAATCGCCGTGGACGCGGTATAGAGAATATTGAACAGAAGCTTGACCAACAACTGCGCCATACGGGCAAGCGTCGGCCAGCTCGGTACGcc > 2:191208/1‑250 (MQ=255) caccaGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTc < 1:149880/251‑1 (MQ=255) caccaGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTc > 1:312465/1‑251 (MQ=255) ggTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATCGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCCTACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAAcc > 2:367888/1‑250 (MQ=255) aTCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCa < 1:440521/251‑1 (MQ=255) cccaccGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGt < 2:418915/250‑1 (MQ=255) ccaccGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGGTGATTGTCTCGCCCACTGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCAGCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCGTGTCCACCACCTGCGCCATTCGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTg < 1:308134/251‑1 (MQ=255) accGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGt > 1:477124/1‑251 (MQ=255) ccGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTc < 1:120528/251‑1 (MQ=255) ccGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTc > 1:222736/1‑251 (MQ=255) cATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTAGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGAACGCCTTCAAACATCAGCGTGGTCGCAcc > 2:57241/1‑251 (MQ=255) cGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGAGTGGCTCTCCCTCGGAACCGAGAATGCGCATCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCTCGCGAATCGCCGTTGGCGCGGTATAGAGAATATTGACCTGAGGCTTGTCCACCACCTGCGCCATACGAGCATGCTACGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCTCCGCAg < 1:432765/248‑1 (MQ=255) gTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTAGAGAGACTATTGACCTGATGCTTGTCCACCACCTGCGCCATAAGTGCAGGCGTCGGCCAGTTCGGTACGATTTAAAACATCAGCGTGGTCGGACCGCAggac > 1:146509/1‑248 (MQ=255) tGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGc > 1:173497/1‑251 (MQ=255) gCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGGTTGGCGCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGc < 2:25121/250‑1 (MQ=255) gATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGc > 1:330535/1‑251 (MQ=255) gATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGc > 1:202/1‑251 (MQ=255) cccAGTACCACTCCCCCGCGTCCTGGTTGATTGGCCCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTAGAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGTTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGc < 2:486784/250‑1 (MQ=255) aCTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGcc < 1:180269/113‑1 (MQ=255) aCTCCCACGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGcc > 2:180269/1‑113 (MQ=255) aCGCTTCCGGGTTGATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCg < 1:149469/251‑1 (MQ=255) gggatgataggcacacccACGGCCCCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCCATCGCTTTATCCCCTTCCGCCATCAGCGCGCGCATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCCGGCGTCGGCCAGCTCGGTACGCCTTCAACCAGCAGCGTGGGCGCACCGCAGGCAAGCGCGCCGTACAGCACGTAACCGTGTCCGGCCAccc > 2:181600/16‑250 (MQ=255) tgaTTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGcc < 1:209174/248‑1 (MQ=255) gagaATGCGCAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGGGCACCAGt > 1:257515/1‑248 (MQ=255) gcgcAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAAGCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGTGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCa < 2:182825/250‑1 (MQ=255) gcgcAGCGACGATCGGTCGGGGCCGTCAATCGCTTTATCCCCGTCCGCCATCAGCGCGCGAATCGCCGTGTGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGGCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCAc < 2:40825/251‑1 (MQ=255) cgcAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCCGCGTGGTCGCACCGCCGGCCAGCGGGCCGTACAGCGAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGGGCAcgagaggaggagggg > 2:399698/1‑237 (MQ=255) cgcAGCGACGATCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGGCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACGGTGTCCGGTCACCCAGCCCACATCGGCGGTGCa < 1:399698/236‑1 (MQ=255) cgacgaTCGGTCGGTGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGgat < 1:450786/251‑1 (MQ=255) acgaTCGGTCGGTGCCTGCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGgatga < 2:127876/251‑1 (MQ=255) cgatcggtcggtGCCTTCCATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAACCATCCGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCACGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCCCCCGTAGATATCCCCCggcttct > 2:182088/5‑246 (MQ=255) cgatcggtcggtGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGgatgat < 1:335907/247‑1 (MQ=255) cgatcggtcggtGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGGTCACCAAGAGATATCACCCGgatgat > 2:355114/5‑251 (MQ=255) gatcggtcggtGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGGAGGTATCACCCGgatgat > 1:112260/4‑250 (MQ=255) cggtcggtGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATc < 1:341882/251‑1 (MQ=255) gtcggtGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCaa < 1:419396/251‑1 (MQ=255) cggtGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGCATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGAAGATATCACCCGGCTGATAATCaaa > 1:169833/1‑250 (MQ=255) cggtGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAg < 1:49275/251‑1 (MQ=255) cggtGCCTTCAATCGCTTTATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAg < 1:271329/251‑1 (MQ=255) ttATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGTTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATTCGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGTTCGCACCTCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGTTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTc < 1:384415/249‑1 (MQ=255) ttATCCCCTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTc < 1:388795/249‑1 (MQ=255) atcccctcccGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCCCCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAgcg < 2:73726/243‑1 (MQ=255) tcccctcccGCCATCAGCTCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATTCGGGCAGGCGTCGGCCAGGTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCCGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAgcg < 1:19367/243‑1 (MQ=255) ccccTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGcgcc < 1:7027/249‑1 (MQ=255) ccTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGcgccgc > 1:82603/1‑251 (MQ=255) cTTCCGCCATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATAAGGGCAGGCATCGGCCCGTTCGGTACGCCTTCAAACATCAGCGTGGACGCACAGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGCCCGGTCACCCAGCCCACATCGGAGGTGCACCCGTAGATATCACCCGGCTGATACTCAAAAACCTATTTAAAGGTCAGCGCcgcg > 1:461310/1‑251 (MQ=255) cctccATCAGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGAGATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTac < 2:406602/248‑1 (MQ=255) aGCGCGCGAATCGCCGTGGGCGCGGTATAGCGAATATTGACCTGATGCTTGTCCACCACCTGCGCAATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCCGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATTTCACCCGGATGATAATCCAAGACATATTTAAAGGt > 1:225185/1‑230 (MQ=255) aGCGCGCGAATCGCCGTGGGCGCGGTATAGAGAATATTGTCCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGTGCCGTACAGCAAGTAACGGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGt < 2:225185/230‑1 (MQ=255) cgcgcgAATCGCCGGGGGCGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTCGGTACGCCTGCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAAc < 2:247540/251‑1 (MQ=255) | CGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCCATACGGGCAGGCGTCGGCCAGTTGGGTACGCCTTCAAACATCAGCGTGGTCGCACCGCAGGCCAGCGGGCCGTACAGCAAGTAACTGTGTCCGGTCACCCAGCCCACATCGGCGGTGCACCAGTAGATATCACCCGGATGATAATCAAAGACATATTTAAAGGTCAGCGCCGCGTACACCAGATAAC > NC_000913/4286042‑4286533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |