breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 972 G→A A212A (GCG→GCA thrA → fused aspartate kinase/homoserine dehydrogenase 1
RA 71,170 T→C S262P (TCG→CCG)  araC → DNA‑binding transcriptional dual regulator AraC
RA 81,044 C→T L305L (CTG→CTA leuB ← 3‑isopropylmalate dehydrogenase
RA 81,102 G→A S286L (TCG→TTG)  leuB ← 3‑isopropylmalate dehydrogenase
RA 85,203 C→T P279L (CCG→CTG)  leuO → DNA‑binding transcriptional dual regulator LeuO
RA 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
JC JC 156,469 IS1 (–) +9 bp coding (407‑415/585 nt) yadN ← putative fimbrial protein YadN
JC JC 168,803 IS1 (+) +9 bp coding (1320‑1328/2244 nt) fhuA → ferrichrome outer membrane transporter/phage receptor
RA 223,560 T→A intergenic (+152/‑211) gmhB → / → rrsH D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA
RA 246,985 C→T P92S (CCG→TCG)  yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 290,892 C→T M87I (ATG→ATA insB3 ← IS1 family protein InsB
RA 292,019 C→T L174L (CTA→TTA)  yagJ → protein YagJ
RA 309,381 C→T D216N (GAT→AAT)  ecpB ← putative fimbrial chaperone EcpB
RA 309,985 T→A G14G (GGA→GGT ecpB ← putative fimbrial chaperone EcpB
JC JC 362,204 IS1 (–) +9 bp coding (968‑976/1254 nt) lacY ← lactose permease
RA 402,007 C→T A87A (GCC→GCT phoA → alkaline phosphatase
RA 410,666 C→T P175S (CCT→TCT)  mak → fructokinase
RA 421,027 C→T R14R (CGC→CGT proY → putative transporter ProY
RA 456,711 C→G P12R (CCC→CGC)  clpP → ATP‑dependent Clp protease proteolytic subunit
RA 472,849 A→G G84G (GGA→GGG glnK → nitrogen regulatory protein PII‑2
RA 495,622 C→T T168I (ACC→ATC)  htpG → chaperone protein HtpG
RA 534,742 G→A G276D (GGT→GAT)  gcl → glyoxylate carboligase
RA 547,891 C→T L404L (CTC→CTT fdrA → putative oxamate carbamoyltransferase FdrA
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 582,597 C→T intergenic (+500/‑278) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 1,073,610 G→A N134N (AAC→AAT rutA ← pyrimidine monooxygenase RutA
RA 1,077,657 C→T R409H (CGC→CAC)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,102,774 C→T T141T (ACG→ACA csgD ← DNA‑binding transcriptional dual regulator CsgD
RA 1,105,847 G→A G10S (GGT→AGT)  ymdB → 2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity
RA 1,123,823 2 bp→AG coding (231‑232/648 nt) grxB ← reduced glutaredoxin 2
RA 1,147,792 T→A S58T (TCG→ACG)  plsX → putative phosphate acyltransferase
RA 1,158,298 C→T L144F (CTT→TTT)  ptsG → glucose‑specific PTS enzyme IIBC component
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,176,122 C→T V232V (GTC→GTT lolC → lipoprotein release complex ‑ inner membrane subunit
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,194,922 G→A T10T (ACC→ACT rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,222,119 G→A intergenic (+2918/+186) ymgF → / ← ymgD inner membrane protein YmgF/PF16456 family protein YmgD
RA 1,222,525 G→A A37V (GCA→GTA)  ymgD ← PF16456 family protein YmgD
RA 1,247,587 G→A intergenic (‑211/+109) treA ← / ← dhaM periplasmic trehalase/dihydroxyacetone kinase subunit M
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
JC JC 1,293,196 IS5 (–) +4 bp intergenic (‑274/‑328) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,976 +T coding (795/1632 nt) oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,301,979 A→C E266D (GAA→GAC oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,380,222 G→A W25* (TGG→TGA ycjU → beta‑phosphoglucomutase
RA 1,381,927 G→A intergenic (+1120/‑20) ycjU → / → ompG beta‑phosphoglucomutase/outer membrane porin G
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
RA 1,407,851 G→A intergenic (‑56/‑138) dgcM ← / → ynaN diguanylate cyclase DgcM/protein YnaN
RA 1,489,491 G→A L420L (CTG→CTA aldA → aldehyde dehydrogenase A
RA 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D
RA 1,609,148 G→A intergenic (‑126/+81) yneE ← / ← uxaB PF01062 family inner membrane protein YneE/tagaturonate reductase
RA 1,629,247 C→T I11I (ATC→ATT ydfZ → putative selenoprotein YdfZ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,748,841 C→T R240K (AGA→AAA)  ydhT ← uncharacterized protein YdhT
RA 1,751,024 C→A intergenic (‑11/+53) ydhX ← / ← ydhW putative 4Fe‑4S ferredoxin‑like protein YdhX/uncharacterized protein YdhW
RA 1,764,017 C→T intergenic (‑8/+1) sufB ← / ← sufA Fe‑S cluster scaffold complex subunit SufB/iron‑sulfur cluster insertion protein SufA
RA 1,795,254 T→G *100S (TAA→TCA)  ihfA ← integration host factor subunit alpha
RA 1,797,083 C→T V288M (GTG→ATG)  pheT ← phenylalanine‑‑tRNA ligase subunit beta
RA 1,848,300 2 bp→TT coding (376‑377/552 nt) ydjA ← putative oxidoreductase YdjA
JC JC 1,879,829 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,932,745 C→T W4* (TGG→TGA eda ← KHG/KDPG aldolase
RA 1,962,172 C→T A67A (GCC→GCT yecT → protein YecT
RA 1,977,215 (A)8→7 noncoding (88/95 nt) motR ← small RNA MotR
RA 1,996,093 G→A intergenic (+51/+17) yecF → / ← sdiA DUF2594 domain‑containing protein YecF/DNA‑binding transcriptional dual regulator SdiA
RA 1,998,749 C→T L244L (CTG→CTA dcyD ← D‑cysteine desulfhydrase
RA 2,017,589 A→C I346L (ATC→CTC)  fliI → flagellar export ATPase FliI
RA 2,028,651 G→A A239V (GCG→GTG)  yedI ← DUF808 domain‑containing inner membrane protein YedI
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,278 G→A intergenic (+160/‑97) msrQ → / → zinT membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
RA 2,052,885 G→A intergenic (+871/‑758) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
JC JC 2,094,553 IS1 (+) +9 bp coding (19‑27/591 nt) hisH → imidazole glycerol phosphate synthase subunit HisH
RA 2,104,024 G→T I162I (ATC→ATA wbbK ← putative glycosyltransferase WbbK
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,142,598 G→A L117L (CTC→CTT udk ← uridine/cytidine kinase
MC JC 2,171,429 Δ5,958 bp IS3‑mediated [gatR]gatY [gatR], gatD, gatB, gatA, gatZ, gatY
RA 2,233,609 C→T Q4* (CAG→TAG)  yeiS → DUF2542 domain‑containing protein YeiS
RA 2,242,247 C→T G241R (GGG→AGG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,247,134 A→C Y467D (TAC→GAC)  lysP ← lysine:H(+) symporter
RA 2,281,855 T→C G408G (GGT→GGC radD → putative DNA repair helicase RadD
RA 2,281,954 C→A N441K (AAC→AAA radD → putative DNA repair helicase RadD
RA 2,353,360 C→T H238H (CAC→CAT glpA → anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA 2,404,402 G→A S71L (TCG→TTG)  nuoB ← NADH:quinone oxidoreductase subunit B
RA 2,531,659 C→T P66L (CCG→CTG)  cysZ → sulfate:H(+) symporter
RA 2,534,506 C→T I147I (ATC→ATT ptsI → PTS enzyme I
RA 2,556,984 G→A T105I (ACC→ATC)  eutC ← ethanolamine ammonia‑lyase subunit beta
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,609,473 G→A L329L (CTG→CTA hyfG → hydrogenase 4 catalytic subunit HyfG
RA 2,744,217 C→T G105D (GGT→GAT)  rplS ← 50S ribosomal subunit protein L19
RA 2,774,947 G→A G544S (GGT→AGT)  yfjW → uncharacterized protein YfjW
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,880,635 T→C L138L (TTA→TTG casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,942,306 G→A Q88* (CAG→TAG)  gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ
RA 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II
RA 3,087,508 C→T P268L (CCA→CTA)  metK → methionine adenosyltransferase
RA 3,134,479 C→T Q117* (CAG→TAG)  yghT → putative ATP‑binding protein YghT
RA 3,167,148 G→A H191Y (CAT→TAT)  ygiS ← putative deoxycholate binding periplasmic protein
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,241,106 A→G N388S (AAC→AGC)  sstT → serine/threonine:Na(+) symporter
RA 3,279,276 C→G F121L (TTC→TTG kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein
RA 3,302,338 C→A A285E (GCG→GAG)  ubiU → ubiquinone biosynthesis protein UbiU
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,411,689 G→T E13* (GAA→TAA)  yhdJ → DNA adenine methyltransferase
RA 3,433,900 C→T L71L (CTA→TTA)  def → peptide deformylase
RA 3,465,502 G→A E141K (GAG→AAG)  gspM → Type II secretion system protein GspM
RA 3,474,424 T→G K43N (AAA→AAC rpsL ← 30S ribosomal subunit protein S12
RA 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,511,466 C→T V293V (GTG→GTA yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,524,913 C→T F681F (TTC→TTT mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,555,047 2 bp→TG coding (1963‑1964/2706 nt) malT → DNA‑binding transcriptional activator MalT
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,581,222 A→G N29D (AAC→GAC)  yhhY → N‑acetyltransferase YhhY
RA 3,599,487 C→T A58T (GCT→ACT)  livJ ← branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein
RA 3,602,471 C→T D93N (GAC→AAC)  ftsE ← cell division protein FtsE
RA 3,618,809 C→T T74T (ACC→ACT nikR → DNA‑binding transcriptional repressor NikR
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,670,329 C→T L246L (CTC→CTT treF → cytoplasmic trehalase
RA 3,685,480 C→T E47K (GAA→AAA)  pdeK ← c‑di‑GMP phosphodiesterase PdeK
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,719,429 G→A intergenic (‑385/‑49) yiaF ← / → yiaG DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,440 2 bp→TT noncoding (105‑106/160 nt) xylZ ← small RNA XylZ
RA 3,730,560 C→T W69* (TGG→TAG)  xylA ← xylose isomerase
RA 3,737,783 C→T T96I (ACC→ATC)  malS → alpha‑amylase
RA 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH
RA 3,772,207 C→T intergenic (‑463/‑74) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,926,991 G→A L5L (CTG→TTG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,966,530 2 bp→TT coding (114‑115/1260 nt) rho → transcription termination factor Rho
JC JC 4,044,847 IS5 (+) +4 bp coding (649‑652/1431 nt) yihF → DUF945 domain‑containing protein YihF
RA 4,081,749 G→A P38S (CCG→TCG)  fdoI ← formate dehydrogenase O subunit gamma
RA 4,095,026 G→A D319D (GAC→GAT rhaA ← L‑rhamnose isomerase
RA 4,096,144 G→A G435G (GGC→GGT rhaB ← rhamnulokinase
JC JC 4,124,494 IS2 (+) +5 bp intergenic (‑38/+114) cytR ← / ← priA DNA‑binding transcriptional repressor CytR/primosomal protein N'
RA 4,133,270 C→T Q219* (CAG→TAG)  metF → 5,10‑methylenetetrahydrofolate reductase
RA 4,138,436 C→T L200L (CTG→CTA gldA ← L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
RA 4,145,137 C→T T381T (ACC→ACT pflD → putative formate acetyltransferase 2
RA 4,166,554 C→T intergenic (+269/‑105) murI → / → rrsB glutamate racemase/16S ribosomal RNA
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,256,705 C→T R23R (CGC→CGT dgkA → diacylglycerol kinase
RA 4,259,570 G→A A145V (GCA→GTA)  zur ← DNA‑binding transcriptional repressor Zur
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,307,944 C→T A179T (GCA→ACA)  alsE ← D‑allulose‑6‑phosphate 3‑epimerase
RA 4,322,288 A→G F45F (TTT→TTC phnF ← putative transcriptional regulator PhnF
RA 4,325,000 C→T G56S (GGC→AGC)  phnC ← phosphonate/phosphate ABC transporter ATP binding subunit
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP
RA 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI
JC JC 4,440,465 IS3 (–) +3 bp coding (749‑751/1344 nt) paeA ← putative cadaverine/putrescine exporter
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,476,605 G→A S390N (AGC→AAC)  yjgL → protein YjgL
RA 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
JC JC 4,542,042 IS1 (+) +8 bp coding (6‑13/597 nt) fimE → regulator for fimA
RA 4,544,413 G→A G37E (GGA→GAA)  fimC → type 1 fimbriae periplasmic chaperone
RA 4,549,043 T→G L79R (CTG→CGG)  fimH → type 1 fimbriae D‑mannose specific adhesin
RA 4,625,303 G→A G137S (GGC→AGC)  serB → phosphoserine phosphatase
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 731314–731469 732187–731990 522–874 29 [27] [27] 28 rhsC rhs element protein RhsC
* * ÷ NC_000913 1196316 1211460 15145 28 [24] [26] 30 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978503–1979270 1979336 67–834 105 [3] [0] 105 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423822–3424233 3424448–3424239 7–627 29 [27] [27] 28 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1397241NA (NA)89 (1.200)
+59 bp
58/466 0.1 97.4% intergenic (+149/‑124) insH4/ynaJ IS5 family transposase and trans‑activator/DUF2534 domain‑containing protein YnaJ
?NC_000913 = 1978502 3 (0.030)intergenic (‑305/+16) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979337 =0 (0.000)106 (1.140) 65/584 0.1 100% intergenic (‑122/‑416) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102943 NA (NA)noncoding (1/1195 nt) IS5 repeat region